Nilamsari, Ruri Vivian (2018) Pengaruh Benzo[a]pyrene terhadap jumlah relatif sel dendritik plasmacytoid CD123+IL-8+ dan Sel Natural Killer pada mencit (Mus musculus) Pasca Vaksinasi Campak. Sarjana thesis, Universitas Brawijaya.
Abstract
Campak merupakan suatu penyakit yang disebabkan oleh virus campak.. Terdapat kejadian di masyarakat bahwa saat balita yang telah divaksinasi campak masih ada anak-anak yang terinfeksi penyakit campak. Hal tersebut diduga karena adanya polutan yang menghambat kerja vaksin campak dalam tubuh. Salah satu senyawa penyusun polutan yaitu Benzo[a]pyrene. Benzo[a]pyerene merupakan senyawa PAH yang merupakan immunosupresi terhadap sel-sel imun. Penelitian ini bertujuan untuk mengetahui pengaruh benzo[a]pyrene terhadap sel dendritik plasmacytoid (CD123) yang mengekspresikan IL-8 dan pada sel Natural killer pada mencit pasca vaksinasi. Pada penelitian ini terdiri dari empat perlakuan yaitu kontrol, benzo[a]pyrene, vaksin dan vaksin benzo[a]pyrene. Mencit diinjeksi vaksin campak dengan dosis 20 mg/kgBB. Kemudian setelah 2 jam diinjeksi dengan benzo[a]pyrene dengan dosis yang sama. Pemberian benzo[a]pyrene terus dilakukan setiap seminggu 2 kali selama 5 minggu. Mencit diisolasi organ spleennya. Selanjutnya diwarnai dengan antibodi ekstraselular dan intraselular dan di analisis menggunakan flow cytometri. Hasil menunjukkan bahwa vaksin dan benzo[a]pyrene dapat memicu respon imun sel dendritik plasmacytoid (CD123) melalui dua signaling yang berbeda dalam mengekspresikan sitokin IL-8 yaitu via NF-κB dan MHC II. Sedangkan pada sel Natural Killer hasil benzo[a]pyrene menunjukkan pengaruh melalui mekanisme IFN tipe I. IFN tipe I ini penting untuk sel NK dalam upaya merespon virus yang masuk kedalam tubuh.
English Abstract
The Measles is a disease caused by measles virus. Symptoms of measles include cough, high fever, watery eyes and accompanied by a rash on the skin. In the prevention of measles, measles vaccination is held. Measles vaccination was performed on a 9-month-on toddler. There is an incident in the community that when children under five have measles vaccination, children are still infected with measles. This is allegedly due to the presence of pollutants that inhibit the action of measles vaccine in the body. One of the pollutant compounds is Benzo[a]pyrene. Benzo[a] pyerene is a PAH compound that is immunosuppressed against immune cells. This study aims to determine the effect of benzo [a] pyrene on dendritic plasmacytoid (CD123) cells expressing IL-8 and on Natural killer cells in post-vaccination in mice. In this study consisted of four treatments namely control, benzo [a] pyrene,vaccine, vaccine and benzo vaccine [a] pyrene. Mice injected measles vaccine at a dose of 20 mg / kgBW. After 2 hours injected with benzo [a] pyrene with the same dose. Benzo [a] pyrene continues to be done every 2 times a weeks for 5 weeks. Mice isolated spleen organ. It was stained with extracellular and intracellular antibodies and analyzed using flow cytometry. The results show that vaccines and benzo [a] pyrene can trigger a plasmacytoid dendritic cell immune response through two different signaling in expressing IL-8 cytokines via NF-κB and MHC II. While in the Natural Killer cell the benzo [a] pyrene results show an influence through a Type I IFN mechanism
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018/142/051805288 |
| Uncontrolled Keywords: | Benzo[a]pyrene, CD123, IL-8, Natural Killer, Vaksin campak Benzo[a]pyrene, CD123, IL-8, Natural Killer, Measles vaccine |
| Subjects: | 500 Natural sciences and mathematics > 571 Physiology and related subjects > 571.9 Diseases > 571.99 Specific communicable diseases |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 17 Jun 2020 23:40 |
| Last Modified: | 16 Mar 2022 07:47 |
| URI: | http://repository.ub.ac.id/id/eprint/168295 |
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