Ikhsani, Hanifah (2018) Uji Efek Pemberian Ekstrak Daun Cocor Bebek (Bryophyllum pinnatum) Terhadap Kadar TNF- α Pada Mencit BALB/C Model Lupus Induksi Pristan. Sarjana thesis, Universitas Brawijaya.
Abstract
Lupus Eritematosus Sistemik suatu penyakit autoimun yang etiologi nya masih belum diketahui dan memiliki manifestasi klinis berbeda di setiap orang. Patogenesis LES ini sangat kompleks, TNF-α dianggap sebagai sitokin utama dalam patogenesis terjadinya LES. TNF-α merupakan sitokin yang berperan dalam proses inflamasi , yang memiliki kemampuan dalam menginduksi kematian atau proliferasi sel, Tujuan pengobatan LES menurunkan aktivitas penyakitnya, mencegah adanya komplikasi, dan mencapai remisi yang permanen. Ada pengobatan yang digunakan untuk LES, cenderung mahal dan menimbulkan efek samping yang cukup banyak. Tujuan penelitian ini untuk mengetahui efek ekstrak etanol daun cocor bebek (Bryophyllum pinnatum) terhadap kadar dari TNF- α pada mencit LES. Penelitian ini eksperimental menggunakan mencit BALB/c betina berusia 6-8 minggu. Mencit dikelompokan menjadi 5 kelompok, (1) Kontrol negatif (2) Kontrol positif (tanpa perlakuan) (3) mencit LES diberikan perlakuan Bryophyllum pinnatum 10,5 mg/kgBB (4) ) mencit LES diberikan perlakuan Bryophyllum pinnatum 21 mg/kgBB (5)mencit LES diberikan perlakuan Bryophyllum pinnatum 42 mg.kgBB. Mencit diinduksi pristane selama 16 minggu dan diberikan ekstrak etanol daun cocor bebek (Bryophyllum pinnatum) selama 12 minggu. Mencit dibedah dan diambil serum darah untuk diperiksakan kadar TNF- α menggunakan metode ELISA. Hasil uji analisis kadar serum TNF-α menggunakan SPSS for windows versi 16.0 dengan uji statistic One Way Anova dan dilanjutkan uji post hoc menggunakan metode LSD. Didapatkan nilai perbedaan signifikan (p=0,000) antara kontrol positif dengan ketiga kelompok perlakuan.
English Abstract
Systematic Lupus Erytematosus is autoimmune disease that have the etiology is unclear and clinical manifestation on human is different. Pathogenesis SLE is complex, this disease marked with production pathogen autoantibody that attack nucleate acid and the protein binding, on this situation can decrease self tolerance overall. TNF-α is the primary cytokine that occur on pathogenesis of SLE. Act as cytokine on inflammatory process, have ability to induce death and proliferative cell. Treatment goals SLE is decrease disease activity, prevent the complication and permanent remission. There is medicine that uses on SLE, but tend to be expensive and raises the side effect especially on long term uses. The purpose on this research is knowing the effect of cocor bebek leaf (Bryophyllum pinnatum) toward TNF- α levels on mice SLE. This research is experimental uses female BALB/c mice aged 6-8 weeks. Mice are grouped into 5 groups. (1) negative control (2) positive control (mice pristane induce lupus) (3) mice induce lupus given extract cocor bebek leaf 10,5 mg/kgBB dose (4) mice induce lupus given extract cocor bebek leaf 21 mg/kgBB dose (5) mice induce lupus given extract cocor bebek leaf 42 mg/kgBB dose. Mice was induce by pristane for 20 weeks and given extract cocor bebek leaf for 12 weeks. Mice do dissection and take the blood serum to check TNF-α levels use ELISA method. Result test of TNF- α levels use SPSS for windows 16.0 version and statistic test One-way ANOVA and continue to post hoc test use LSD method. There is significant difference value (p=0,000) between positive control with all three groups.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2018/558/051901131 |
| Uncontrolled Keywords: | Lupus Eritematosus Sistemik, TNF-α, daun cocor bebek, mencit, pristane, Systemic Lupus Erytematosus, TNF- α, cocor bebek leaf, mice, pristane |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 72 Drugs derived from specific plants (Crassulaceae) |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Nur Cholis |
| Date Deposited: | 10 Jul 2019 02:06 |
| Last Modified: | 21 Oct 2021 03:22 |
| URI: | http://repository.ub.ac.id/id/eprint/168033 |
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