Kuncoro, Adi (2018) Pengaruh Pemberian Quercetin Terhadap Degranulasi Sel Mast Otak Pada Tikus Wistar yang Diberi Stres Subakut. Sarjana thesis, Universitas Brawijaya.
Abstract
Stres diketahui berperan dalam proses terjadinya beberapa kondisi patologis dalam tubuh manusia, seperti gangguan tidur, migraine dan kondisi lainnya dimana mekanismenya berkaitan dengan proses pengeluaran mediator inflamasi oleh sel mast otak yang mengalami degranulasi. Quercetin diketahui dapat mencegah degranulasi sel mast perifer. Quercetin juga diketahui dapat menembus sawar darah otak. Penelitian ini dilaksanakan untuk mengevaluasi efek dari pemberian quercetin intraperitoneal terhadap degranulasi sel mast otak tikus yang mengalami paparan stres subakut audiogenik berupa white noise berdurasi 4 jam per hari selama 14 hari. Sel mast diidentifikasi dengan menggunakan pewarnaan toluidine blue pada regio talamus. 6 tikus digunakan pada setiap kelompok perlakuan. Pada kelompok perlakuan injeksi intraperitoneal quercetin didapatkan jumlah sel mast dan degranulasi sel mast yang lebih sedikit dibandingkan dengan kelompok kontrol (kelompok yang mendapat paparan stres namun tidak mendapat injeksi quercetin intraperitoneal) (p<0.05). Dapat disimpulkan bahwa injeksi intraperitoneal quercetin dapat mempengaruhi degranulasi sel mast otak.
English Abstract
Stress is known to be involved in several human pathological conditions, such as sleeping disorder, migraine, etc. One of the mechanisms involved is the secretion of the inflammatory mediator by the brain mast cell that undergoes degranulation in response to stress. Quercetin is known to prevent the degranulation of peripheral mast cells. Quercetin is also known to cross the blood-brain barrier. This present study was set out to evaluate the effect of quercetin intraperitoneal injection on the brain mast cells degranulation after 4-h daily exposure to white noise stress for 14 days. Mast cell s were identified with touluidine blue staining in the thalamus. Six male rats were used for each of the groups. The study shows that the amount and percentage of mast cell degranulation is smaller in rats given intraperitoneal quercetin injection compared the rats in the control group (stressed rats without quercetin administration) (P<0.05). Thus, it can be concluded that intraperitoneal quercetin administration alter the amount and degranulation of brain mast cells.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2018/91/051804086 |
| Uncontrolled Keywords: | stres, sel mast, quercetin, stress, mast cell, quercetin |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Nur Cholis |
| Date Deposited: | 21 May 2020 06:19 |
| Last Modified: | 21 Oct 2021 05:03 |
| URI: | http://repository.ub.ac.id/id/eprint/168020 |
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