Putri, Ni Luh Ayu Pradnyani (2018) Perbandingan Pemberian Pakan Diet Tinggi Lemak Terhadap Gambaran Histopatologi Hepar Dan Kadar Kolesterol Tikus (Rattus Norvegicus) Yang Diberi Paparan Immobilization Stress. Sarjana thesis, Universitas Brawijaya.
Abstract
Kondisi hiperkolesterolemia dipicu oleh gaya hidup masyarakat yang gemar mengkonsumsi makanan cepat saji yang tinggi akan lemak. Hal ini memicu kondisi hiperkolesterolemia dan disertai dengan adanya stress yang memiliki potensi resiko radikal bebas dan kerusakan pada hepar. Penelitian ini bertujuan mengetahui pengaruh pemberian pakan diet tinggi lemak terhadap gambaran hitopatologi hepar dan kadar kolesterol tikus (Rattus norvegicus) yang diberi paparan immobilization stress. Penelitian ini menggunakan tikus (Rattus norvegicus) dibagi dalam 4 kelompok perlakuan yaitu: Kelompok tikus sehat, kelompok perlakuan 1 diberi perlakuan immobilization stress, kelompok perlakuan 2 diberi diet tinggi lemak sebanyak 5 mL/ekor, kelompok perlakuan 3 diberi diet tinggi lemak 5 mL/ekor dan immobilization stress,. Pemberian pakan diet tinggi lemak diberikan dengan metode sonde lambung sebanyak 3 kali sehari mulai hari ke 8-12 hari dengan waktu pemberian pagi, siang dan sore hari. Perlakuan immobilization stresss dilakukan dengan cara memasukkan tikus ke dalam chamber dengan ukuran 30 cm x 10 cm. Perlakuan immobilization stress dilakukan 2 kali sehari, yaitu pada pukul 08.00-11.00, kemudian diistirahatkan selama 2 jam (11.00-13.00) dan diberikan perlakuan stress kembali pukul 13.00-16.00. Kadar kolesterol total pada tikus diukur dengan metode spektrofotometri, data dianalisa dengan uji ANOVA dilanjutkan uji Tukey (p<0,05). Gambaran histopatologi hepar diamati secara kualitatif dengan pewarnaan HE. Hasil penelitian immobilization stress tidak memberikan pengaruh terhadap peningkatan kadar kolesterol, perlakuan kombinasi diet tinggi lemak dan immobilization stress dapat meningkatkan kadar kolesterol. Kesimpulannya paparan immobilization stress tidak berpengaruh terhadap kadar kolesterol dan pemberian pakan diet tinggi lemak mempengaruhi kadar kolesterol dan gambaran histopatologi hepar yang sangat signifikan.
English Abstract
The condition of hypercholesterolemia is triggered by the lifestyle of people who like to consume fast food that is high in fat. This triggers the condition of hypercholesterolemia and is accompanied by stress which has the potential risk of free radicals and damage to the liver. This research purpose to determine the effect of feeding a high-fat diet on histopathology of the liver and cholesterol levels of rat (Rattus norvegicus) given exposure to immobilization stress. This study used rat (Rattus norvegicus) divided into 4 treatment groups, there are Group of healthy rat, treatment group 1 treated with immobilization stress, treatment group 2 was given a high fat diet of 5 ml / rat, treatment group 3 was given a high-fat 5 mL and immobilization stress. The feeding process using stomach tube method 3 times a day from the 8-12 day to the time of the morning, afternoon and evening. The immobilization stress treatment work with putting rat in chamber with size 30 x 10 cm. The immobilization stress treatment is carried out twice a day, namely at 08.00-11.00, then rested for 2 hours (11.00-13.00) and given a stress treatment again at 13.00-16.00. Total cholesterol levels in rat were measured by spectrophotometric methods, data were analyzed by ANOVA test followed by Tukey test (p <0.05). Liver histopathology is observed qualitatively with HE staining. The results of immobilization stress studies have no effect on increasing cholesterol levels, the combination treatment of high-fat diets and immobilization stress can increase cholesterol levels. In conclusion, immobilization stress exposure had no effect on cholesterol levels and feeding a highfat diet affected cholesterol levels and very significant liver histopathology.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/203/051900932 |
| Uncontrolled Keywords: | Tinggi lemak, Hiperkolesterolemia, Rattus norvegicus, Kolesterol, Histopatologi, Hepar./ High fat, Hypercholesterolemia, Rattus norvegicus, Cholesterol, Histopathology, Liver |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine > 636.089 32 Veterinary medicine--Nutritional aspects |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Endang Susworini |
| Date Deposited: | 29 May 2020 14:24 |
| Last Modified: | 29 May 2020 14:24 |
| URI: | http://repository.ub.ac.id/id/eprint/168010 |
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