Rozzaaq. As, Regi Abdul (2018) Pengaruh Suplementasi Ephitelia Cell Oviduct Glycoprotein (Oviductin) Pada Media Maturasi Terhadap Tingkat Maturasi Oosit Secara In Vitro dan Ekspresi Ephitelia Cell Oviduct Glycoprotein (Oviductin) Pada Ovum Secara Imunositokimia. Sarjana thesis, Universitas Brawijaya.
Abstract
Oviductin diketahui dapat meningkatkan maturasi oosit dan meminimalisasi terjadinya polispermi pada kambing. Melihat potensi dari oviductin tersebut maka dilakukan penelitian tentang pengaruh suplementasi oviductin pada media maturasi terhadap tingkat maturasi oosit dan mengurangi kejadian polispermi sehingga membantu meningkatkan populasi ternak kambing di Indonesia. Penelitian ini bertujuan untuk mengetahui pengaruh oviductin pada tingkat maturasi oosit secara in vitro dan ekspresi oviductin pada ovum. Oosit dari ovarium kambing yang berasal dari limbah rumah potong hewan (RPH), dikelompokkan berdasarkan kontrol 0 mg/ml, 5 mg/ml, dan 10 mg/ml. Oosit diaspirasi dari ovarium dengan syringe 10 ml dan jarum 18 G. Oosit dengan kualitas A yang digunakan dalam penelitian ini. Oosit dikultur pada media maturasi. 50μl drop dan dilapisi minyak mineral, diinkubasi pada suhu 38,5ºC, CO2 5% selama 24 jam. Tingkat maturasi in vitro ditentukan dengan pewarnaan aceto orcein untuk melihat tahapan maturasi berdasarkan germinal vesicle, germinal vesicle break down, metafase I dan metafase II. Ekspresi oviductin dilihat dengan metode Imunositokimia. Suplementasi oviductin berpengaruh terhadap tingkat maturasi oosit secara signifikan pada dosis 5 μg/ml, 10 μg/ml dan dapat miningkatkan maturasi oosit secara signifikan. Untuk ekspresi oviductin terdapat pada perivitelin space, zona pelusida dan kumulus oophorus (COC).
English Abstract
Oviductin is known to increase oocyte maturation and minimize polyspemi in goats. Considering the potential of oviductin, research on the effect of oviductin supplementation on medium maturation to maturation rate and reduce the incidence of polispermi thus helping to increase the population of goats in Indonesia. This study was conducted to determine the expression of oviductin in ovum and the effect of oviductin supplementation on media maturation to in vitro maturation rate. Oocyte from goats ovaries that come from slaughterhouses, grouped control 0 mg/ml, 5 mg/ml and 10 mg/ml. Each treatment group required repetition six time. Oocyte were aspirated from ovaries with a 10 ml syringe fitted with an 18 gauge needle. Oocyte complexes quality A were used for this research. Oocyte were cultured in the maturation medium G-MOPS droplets 50μl and under a layer of mineral oil at a 38.5° C, with 5% CO2 humidity for 24 hours. In vitro maturation rate was determined by staining with aceto orcein to see stage of oocyte maturation by Germinal vesicle, Germinal vesicle breakdown, Metaphase I and Metaphase II. The expression oviductin were observed using immunohistochemistry method. This study research was to determine effect oviductin in vitro maturation rate and expression in ovum. Oviductin supplementation significantly affected maturation rate at doses 10 μg/ ml, 5 μg/ml and maturation rate oocyte significan. oviductin for expression present in zona pellusida, perivitellin space and cumulus oophorus (COC).
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/59/051805226 |
| Uncontrolled Keywords: | Oviductin, in vitro tingkat maturasi, polispermi, perivitelin space, zona pelusida, kumulus oophorus (COC), Imunositokimia |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine > 636.089 239 8 Veterinary medicine--Proteins--Metabolism |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Endang Susworini |
| Date Deposited: | 27 May 2020 14:59 |
| Last Modified: | 27 May 2020 14:59 |
| URI: | http://repository.ub.ac.id/id/eprint/167968 |
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