Nicolas, Franklyn Cristevan (2018) Efektivitas Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi) sebagai Antibakteri terhadap Aggregatibacter Actinomycetemcomitans Pembentuk Biofilm secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Periodontitis agresif merupakan penyakit pada jaringan penyangga gigi yang memiliki karakteristik berupa kerusakan ligamen periodontal dan tulang alveolar yang cepat. Bakteri yang berperan penting dalam menyebabkan periodontitis agresif adalah Aggregatibacter actinomycetemcomitans. Tujuan penelitian ini adalah untuk mengetahui efektivitas ekstrak etanol daun belimbing wuluh (Averrhoa bilimbi) sebagai antibakteri terhadap Aggregatibacter actinomycetemcomitans pembentuk biofilm secara in vitro. Ekstraksi daun belimbing wuluh (Averrhoa bilimbi) menggunakan metode maserasi dengan pelarut etanol 70%. Daun belimbing wuluh (Averrhoa bilimbi) mengandung zat aktif yang mempunyai efek antibakteri yaitu flavonoid, saponin, dan tannin. Penelitian ini menggunakan desain true experimental dengan metode difusi sumuran, yang digunakan adalah konsentrasi ekstrak etanol daun belimbing wuluh (Averrhoa bilimbi) 12,5%, 25%, 50%, 70%, dan 100%. Hasil penelitian menunjukkan bahwa pada konsentrasi ekstrak 25% sudah mampu menghambat pertumbuhan bakteri Aggregatibacter actinomycetemcomitans dengan terbentuknya zona hambat pertumbuhan bakteri pada media Nutrient agar (NA) dengan respon hambat lemah. Analisis data dilakukan dengan menggunakan uji statistik One-Way Analysis of Variance (ANOVA) dan uji korelasi-regresi. Hasil uji statistik menunjukkan bahwa terdapat perbedaan efek antibakteri yang signifikan pada setiap pemberian konsentrasi ekstrak etanol daun belimbing wuluh terhadap pertumbuhan bakteri Aggregatibacter actinomycetemcomitans serta terdapat hubungan yang kuat antara pemberian ekstrak etanol daun belimbing wuluh dengan pertumbuhan bakteri Aggregatibacter actinomycetemcomitans (p<0,05). Kesimpulan dari penelitian ini adalah ekstrak etanol daun belimbing wuluh (Averrhoa bilimbi) efektif sebagai antibakteri terhadap bakteri Aggregatibacter actinomycetemcomitans pembentuk biofilm secara in vitro dengan kadar hambat minimum pada konsentrasi 25%.
English Abstract
Aggressive Periodontitis is a disease of dental supporting tissue which has characteristic such as rapid periodontal ligament and alveolar bone damage. The bacteria that play an important role in causing aggressive periodontitis is Aggregatibacter actinomycetemcomitans. The purpose of this research was to determine the effect of ethanol extract of bilimbi (Averrhoa bilimbi) leaves as an antibacterial against Aggregatibacter actinomycetemcomitans in vitro. The extraction of bilimbi (Averrhoa bilimbi) leaves used maceration method with 70% ethanol as the solvent. bilimbi (Averrhoa bilimbi) leaves contain active substances that have antibacterial effect such as flavonoids, saponins and tannins. This research is using true experimental design with well diffusion method, and using 12,5%, 25%, 50%, 70%, and 100% concentration of ethanol extract of bilimbi (Averrhoa bilimbi) leaves. The result showed that the extract concentration of 25% was able to inhibit the Aggregatibacter actinomycetemcomitans colonies growth with the formation of inhibition zone of bacterial growth on Nutrient Agar (NA) media which indicate a weak inhibitory response. The data analysis used One-Way Analysis of Variance (ANOVA) and correlation-regression test. The statistical analysis showed that there were significant differences of antibacterial effect on each concentration of ethanol extract of bilmbi leaves to the Aggregatibacter actinomycetemcomitans colonies growth and also there was a strong relation between the ethanol extract of bilimbi leaves and the Aggregatibacter actinomycetemcomitans colonies growth (p <0.05). The conclusion of this research is that the ethanol extract of bilimbi (Averrhoa bilimbi) leaves effective as antibacterial against Aggregatibacter actinomycetemcomitans which Form biofilm in vitro with minimum inhibitory concentration at a concentration of 25%.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKG/2018/47/051808339 |
| Uncontrolled Keywords: | Periodontitis agresif, Aggregatibacter actinomycetemcomitans, daun belimbing wuluh (Averrhoa bilimbi), antibakteri. Aggressive Periodontitis, Aggregatibacter actinomycetemcomitans, Bilimbi (Averrhoa bilimbi) leaves, Antibacterial. |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 79 Drugs derived from spesific plant (Oxalidaceae) |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 16 Jul 2019 04:15 |
| Last Modified: | 22 Oct 2021 14:20 |
| URI: | http://repository.ub.ac.id/id/eprint/167849 |
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