Andani, Nurul Putri (2018) Pengaruh Kombinasi MSG dan Diet Tinggi Lemak terhadap Siklus Estrus pada Tikus Putih (Rattus novergicus Strain Wistar) Betina. Sarjana thesis, Universitas Brawijaya.
Abstract
Efek negatif gaya hidup terkait kesehatan yang muncul adalah meningkatnya obesitas dan konsumsi makanan cepat saji yang dapat mempengaruhi fertilitas. Obesitas seringkali dikaitkan dengan konsumsi diet tinggi lemak yang berlebihan dan makanan cepat saji dikaitkan dengan kandungan penyedap rasa atau Monosodium Glutamate (MSG) yang tinggi. Diet tinggi lemak (DTL) dan MSG secara terpisah telah terbukti dapat mempengaruhi infertilitas dan organ reproduksi wanita, namun belum diteliti pengaruh kombinasi MSG dan diet tinggi lemak terhadap reproduksi wanita khusunya ke siklus estrus tikus yang analog dengan siklus menstruasi manusia. Perubahan siklus estrus yang teramati dapat menjadi tanda adanya gangguan produksi hormon reproduksi. Penelitian ini dilakukan selama 56 hari dan menggunakan tikus Rattus novergicus strain Wistar betina yang dibagi ke 6 kelompok yaitu kontrol negatif, kontrol +1 DTL, kontrol +2 MSG (0,7 mg/grBB), P1 (DTL + MSG 0,05 mg/grBB), P2 (DTL + MSG 0,2 mg/grBB), P3 (DTL + MSG 0,35 mg/grBB). Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan yang signifikan jumlah siklus estrus selama 56 hari antara kelompok perlakuan kombinasi MSG dan DTL dengan kelompok kontrol positif maupun kelompok negatif (p = 0,673). Rata-rata sejumlah 25-50% sampel dari semua kelompok kontrol positif dan kelompok perlakuan kombinasi MSG dan DTL menunjukkan pemanjangan siklus estrus. Selain itu kombinasi MSG dan DTL dapat memunculkan siklus estrus yang ireguler pada 25-50% sampel pada siklus ke-1 hingga siklus ke-6, namun menjadi reguler kembali pada siklus ke-7 hingga siklus ke-14.
English Abstract
The negative health-related lifestyle effects that arise are the increasing obesity and consumption of fast food that can affect fertility. Obesity is often associated with excessive consumption of high-fat diets (HFD) and fast food is associated with high flavor content or Monosodium Glutamate (MSG). HFD and MSG separately have been shown to affect infertility and female reproductive organs, but have not studied the effect of combination of MSG and HFD on women's reproduction especially to the rat estrous cycle analogous to the human menstrual cycle. Observed changes in estrous cycle can be a sign of a disruption of reproductive hormone production. The study was conducted for 56 days and used the female Rattus novergicus wistar strains were divided into 6 groups: negative control, control +1 HFD, control +2 MSG (0.7 mg/grBB), P1 (HFD + MSG 0.05 mg/grBB), P2 (HFD + MSG 0.2 mg/grBB), P3 (HFD + MSG 0.35 mg/grBB). The results showed that there was no significant difference in the number of estrous cycles between treatment groups of MSG and HFD combination with positive control and negative control (p = 0.673). An average of 25-50% of samples from all positive control groups and combination treatment groups of MSG and DTL showed an elongation of the estrous cycle. Furthermore, the combination of MSG and DTL can lead an iregular estrous cycle at 25-50% of samples in the 1st cycle to the 6th cycle but become reguler again in the 7th cycle to the 14th cycle.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2018/34/051803250 |
| Uncontrolled Keywords: | MSG, diet tinggi lemak, siklus estrus, MSG, high-fat diet, estrous cycle |
| Subjects: | 600 Technology (Applied sciences) > 618 Gynecology, obstetrics, pediatrics, geriatrics > 618.1 Gynecology > 618.17 Functional and systemic disorders > 618.172 Menstruation disturbances > 618.172 065 4 Menstruation disorders--Nutritional aspects / Menstruation disorders--Diet therapy |
| Divisions: | Fakultas Kedokteran > Kebidanan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 08 May 2020 12:13 |
| Last Modified: | 19 Oct 2021 05:28 |
| URI: | http://repository.ub.ac.id/id/eprint/167372 |
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