Putri., Margaretha Christina (2018) Pengaruh Ekstrak Etanol Alga Merah (Eucheuma Cottonii) Dan Alga Cokelat (Sargassum Sp.) Terhadap Kadar Malondialdehid Dan Superoksida Dismutase Tikus Yang Diinduksi Stres Oksidatif Dengan Diet Tinggi Sukrosa. Sarjana thesis, Universitas Brawijaya.
Abstract
Gula merupakan bahan tambahan pangan yang dikonsumsi dalam jumlah besar oleh masyarakat Indonesia. Tingginya kebutuhan gula merupakan indikasi tingginya konsumsi gula di Indonesia yang dapat memberikan dampak buruk bagi kesehatan. Efek negatif terhadap kesehatan yang dapat terjadi adalah overweight dan obesitas, yang dapat memicu penyakit yang lebih serius seperti resistensi hormon insulin dan respon inflamasi yang disebabkan karena terjadinya stres oksidatif. Salah satu komoditas pangan dari perairan yang dikenal memiliki aktivitas antioksidan dan antiinflamasi, serta belum banyak dimanfaatkan yaitu rumput laut, contohnya Sargassum sp. dan Eucheuma cottonii yang memiliki kandungan fenolik di dalamnya. Pada penelitian ini diamati efek pemberian ekstrak E. cottonii dan Sargassum sp. terhadap kadar malondialdehid (MDA), suatu marker peroksidasi lipid dan kadar enzim antioksidan superoxide dismutase (SOD) serum darah tikus yang diinduksi stress oksidatif dengan diet tinggi sukrosa. Tikus dibagi ke dalam empat (4) kelompok perlakuan pakan: pakan standar (tikus normal), pakan tinggi sukrosa (tikus stres oksidatif), pakan tinggi sukrosa + ekstrak E. cottonii dan pakan tinggi sukrosa + ekstrak Sargassum sp. Perlakuan diberikan selama 28 hari, dan pada akhir percobaan, tikus dilakukan euthanasia dan diambil darah dari jantung untuk analisa MDA dan SOD serum. Hasil penelitian menunjukkan, total fenol pada E. cottonii sebesar 118,64 ± 30,59 mg PGE/ g ekstrak sedangkan pada Sargassum sp. sebesar 122,54 ± 43,41 mg PGE/ g ekstrak. Pemberian ekstrak E. cottonii dan Sargassum sp. berpengaruh nyata (α=0.05) pada kadar MDA serum tikus yang diinduksi stress oksidatif dengan diet tinggi sukrosa masing-masing sebesar 2,12 ± 0,13 dan 1,76 ± 0,17 ng/100 μl. Lebih rendah dibandingkan kadar MDA serum darah tikus kontrol stress oksidatif tanpa pemberian ekstrak alga (2,49 ± 0,21 ng/100 μl) namun masih belum serendah kadar MDA tikus kontrol sehat/normal (1,38 ± 0,17 ng/100 μl). Pemberian ekstrak E. cottonii dan Sargassum sp. juga berpengaruh nyata (α=0.05) pada kadar SOD serum tikus yang diinduksi stress oksidatif dengan diet tinggi sukrosa masing-masing sebesar 38,65 ± 2,06 dan 38,53 ± 0,34 unit/100 μl. Lebih tinggi dibandingkan kadar SOD serum tikus kontrol stress oksidatif tanpa pemberian ekstrak alga (21,80 ± 1,33 unit/100 μl) namun masih lebih rendah dibandingkan kadar SOD tikus kontrol sehat/normal (46,43 ± 4,67 unit/100 μl). Sehingga dapat disimpulkan bahwa ekstrak E. cottonii dan Sargassum sp. mampu menurunkan kadar MDA dan meningkatkan SOD serum tikus yang diinduksi stres oksidatif dengan diet tinggi sukrosa.
English Abstract
Sugar is an important ingredient commonly used in foods and beverages. Indonesians tend to consume sugar in a significant amount daily, as indicated by high-demand of sugar in the market. In the long-term, high consumption of sucrose will lead to an adverse health effect such as overweight and obesity, insuline resistance as well as inflammatory response caused by severe oxidative stress. One of the marine resources known to have antioxidant and antiinflammatory activity, but not yet widely used is algae, namely Eucheuma cottonii and Sargassum sp. Several studies have shown that Eucheuma cottonii and Sargassum sp. can reduce oxidative stress due to its phenolic compounds. The present study observed the effect of Eucheuma cottonii extract and Sargassum sp. extract on Malondialdehyde (MDA) and Superoxide Dismutase Enzyme (SOD) level in serum of stress oxidative-induced rats with high sucrose diet. Rats were randomly assigned into 4 groups differing in the type of diet: standard diet (normal group), diet with high content of sucrose (oxidative-stress group), diet with high sucrose + E. cottonii extract and diet with high content of sucrose + Sargassum sp. extract. The treatment was given for 28 days (4 weeks). At the end of the experiment, blood was collected by means of cardiac puncture for analysis of MDA and SOD serum. The results showed that total phenolic compound in Eucheuma cottonii and Sargassum sp. extract were 118,64 ± 30,59 mg PGE/g extract and 122,54 ± 43,41 mg PGE/g extract respectively. Animal study showed that both extracts significantly affected (α=0.05) MDA level in serum of stress oxidative-induced rats with high sucrose diet as much as 2,12 ± 0,13 and 1,76 ± 0,17 ng/100 μl respectively. The MDA level were lower than that in the control group of rats induced stress oxidative without given an algae extract (2,49 ± 0,21 ng/100 μl) but still higher than that in the control group of normal rats (1,38 ± 0,17 ng/100 μl). Furthermore, both extracts were able to increase significantly (α=0.05) SOD level in serum of oxidative stress-induced rats as much as 38,65 ± 2,06 and 38,53 ± 0,34 unit/100 μl respectively. The SOD level was higher than that in the control group of rats induced stress oxidative without given an algae extract (21,80 ± 1,33 unit/100 μl) but still lower than that in the control group of normal rats (46,43 ± 4,67 unit/100 μl). E. cottonii and Sargassum sp. ethanolic extract were able to decrease MDA level and increase SOD level in serum of oxidative stress-induced rats with high sucrose diet. In conclusion, E. cottonii and Sargassum sp. ethanolic extracts were able to decrease MDA levels and to increase SOD levels in stress-oxidative induced rats with high sucrose diet.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FTP/2018/342/051809015 |
| Uncontrolled Keywords: | Diet tinggi sukrosa, Eucheuma cottonii, Sargassum sp., Stres oksidatif,/ Eucheuma cottonii, High-sucrose diet, Oxidative stress, Sargassum sp. |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.329 89 Drugs derived from microorganism, fungi, algae (Rhodophyta (Red algae)) |
| Divisions: | Fakultas Teknologi Pertanian > Teknologi Hasil Pertanian |
| Depositing User: | Endang Susworini |
| Date Deposited: | 10 Jul 2019 02:39 |
| Last Modified: | 30 Jun 2022 04:52 |
| URI: | http://repository.ub.ac.id/id/eprint/165375 |
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