Hayati, Asna Ainul (2018) Potensi Konsorsium Rizobakteri Dari Tumbuhan Solanaceae Di Ub Forest Sebagai Bioprotektan Dan Biostimulan Pada Tanaman Tomat. Sarjana thesis, Universitas Brawijaya.
Abstract
Tomat merupakan salah satu komoditas hortikultura yang banyak diminati dan memiliki nilai ekonomi tinggi. Kendala terhadap produksi tanaman tomat salah satunya diakibatkan oleh serangan patogen R. solanacearum penyebab penyakit layu bakteri pada tanaman tomat. Pengendalian yang dapat dilakukan yaitu dengan menggunakan konsep PHT, berupa pemanfaatan agens hayati. Agens hayati dalam penelitian ini diisolasi dari daerah perakaran tumbuhan Solanaceae di UB forest yaitu cepoka dan kecubung. Penelitian ini bertujuan untuk mendapatkan isolat rizobakteri dalam bentuk tunggal dan konsorsium untuk mengendalikan penyakit layu bakteri dan mampu meningkatkan pertumbuhan tanaman tomat. Penelitian dilakukan pada bulan Maret sampai Oktober 2018 di Laboratorium Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya dan di lahan pengamatan yang berada di Desa Dadaprejo, Junrejo, Kota Batu. Hasil eksplorasi dilakukan seleksi sebagai agens antagonis dan PGPR. Karakteristik dari bakteri PGPR ialah bakteri yang mampu melarutkan fosfat dan menambat N. Tiga isolat bakteri terpilih dilakukan uji dalam bentuk isolat tunggal dan formulasi konsorsium, serta perlakuan kontrol negatif menggunakan aquades steril dan kontrol positif dengan bakterisida streptomisin sulfat 20%. Pengujian dilakukan secara in vitro dengan parameter pengamatan berupa kemampuan penghambatan rizobakteri terhadap R. solanacearum dan secara in vivo berupa pengamatan persentase kejadian penyakit, efektivitas mikroba serta pertumbuhan tanaman tomat. Penelitian menggunakan Rancangan Acak Kelompok (RAK) dengan 9 perlakuan dan 4 ulangan. Hasil penelitian menunjukkan bahwa tiga isolat bakteri yang telah diidentifikasi yaitu isolat bakteri KC 5 diketahui sebagai Bacillus sp., KC 20 merupakan Pseudomonas sp., dan CP 20 diketahui berasal dari genus Pantoea sp. Dari hasil pengujian secara in vitro diketahui konsorsium mikroba antagonis secara nyata mampu menghambat pertumbuhan patogen R. solanacearum, akan tetapi tidak memberikan pengaruh dalam menekan kejadian penyakit layu bakteri secara in vivo. Sementara itu, hasil penelitian terhadap pertumbuhan tanaman tomat di lapang menunjukkan perlakuan konsorsium antara Pseudomonas sp. dan Pantoea sp. mampu meningkatkan tinggi tanaman dan jumlah daun. Sedangkan untuk perlakuan isolat tunggal, hasil terbaik ditunjukkan oleh perlakuan Bacillus sp. dalam meningkatkan tinggi tanaman dan isolat Pantoea sp. dalam meningkatkan jumlah daun tanaman tomat.
English Abstract
Tomatoes are one of the most popular horticultural commodities and have high economic value. One of the constraints on the production of tomato plants is caused by the attack of the pathogen R. solanacearum which causes bacterial wilt in tomato plants. Control that can be done is by using the concept of IPM, in the form of utilization of biological agents. The biological agent in this study was isolated from the Solanaceae plant roots in UB forest, namely cepoka and kecubung. This study aims to obtain rhizobacterial isolates in a single form and consortium to control bacterial wilt disease and to increase the growth of tomato plants. The study was conducted in March to October 2018 at the Plant Disease Laboratory of the Faculty of Agriculture, University of Brawijaya and on the observation field located in Dadaprejo Village, Junrejo, Batu City. The exploration results were selected as antagonist agents and PGPR. Characteristics of PGPR bacteria are bacteria capable of dissolving phosphate and anchoring N. Three selected bacterial isolates were tested in the form of single isolates and consortium formulations, and negative control treatment using sterile distilled water and positive control with bactericidal streptomycin sulfate 20%. Tests were carried out in vitro with observational parameters in the form of the ability to inhibit rhizobacteria against R. solanacearum and in vivo by observing the percentage of disease incidence, microbial effectiveness and growth of tomato plants. The study used a Randomized Block Design (RBD) with 9 treatments and 4 replications. The results showed that three bacterial isolates identified were KC 5 bacterial isolates known as Bacillus sp., KC 20 were Pseudomonas sp., And CP 20 was known to originate from the genus Pantoea sp. From the results of in vitro testing it is known that the consortium of microbial antagonists is actually able to inhibit the pathogen growth of R. solanacearum, but it has no effect in suppressing the incidence of bacterial wilt in vivo. Meanwhile, the results of research on the growth of tomato plants in the field show the treatment of the consortium between Pseudomonas sp. and Pantoea sp. able to increase plant height and number of leaves. As for the treatment of single isolates, the best results were shown by the treatment of Bacillus sp. in increasing plant height and isolates of Pantoea sp. in increasing the number of leaves of tomato plants.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2018/977/051901390 |
| Uncontrolled Keywords: | Tomat |
| Subjects: | 500 Natural sciences and mathematics > 583 Magnoliopsida (Dicotyledons) > 583.9 Asteridae > 583.95 Scrophulariales > 583.952 Solanaceae |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Nur Cholis |
| Date Deposited: | 05 Jul 2019 02:10 |
| Last Modified: | 20 Oct 2021 04:01 |
| URI: | http://repository.ub.ac.id/id/eprint/165187 |
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