Ashlina, Nadila Dwi (2018) Pengaruh Pemberian Salep Kitosan Ekstrak Cangkang Ranjungan (Portunus pelagicus) 5% Pada Tikus Putih (Rattus norvegicus) yang diinduksi Luka Bakar terhadap EkspresiVEGF dan Histopatologi Kulit. Sarjana thesis, Universitas Brawijaya.
Abstract
Lukabakaradalahsuatu trauma yang disebabkanolehapi, aruslistrik, bahankimia, radiasi, danpetir yang mengenaikulit, mukosamaupunjaringan. Kitosan yang terkandung dalam cangkang rajungan (Portunus pelagicus) dapat digunakan sebagai salah satu alternatif pengobatan luka bakar yang berpotensiuntukmeningkatkan proliferasi sel, reepitelisasi, angiogenesis, dananti bakteri. Penelitian ini bertujuan untuk mengetahui pengaruh terapi salep kitosan ekstrak cangkang rajungan (Portunus pelagicus) terhadap luka bakar tikus putih terhadapmakrofag yangmengekspresikanVEGF (vascular endothelial growth factor)danhistospatologikulit.Penelitianinimenggunakan 24 ekortikusputih (Rattusnovergicus) umur 2-3 bulan, danberatbadan<150 g denganrancanganacakkelompokfaktorial (RAKF)3 kelompok perlakuan yaitukelompok kontrolekstrak placenta danNeomysinsulfatdanperlakuankitosan 5% selama 7 harisecaratopikaldua kali sehari. Pengambilansampeldilakukanhari ke-1, 3,dan 7 setelahperlakuan.PengamatanekspresiVEGF menggunakan metode imunohistokimia (IHK)dengan menghitung jumlah sel makrofag yang terekspresidanhistopatologikulitdenganpewarnaan Hematoxylin-Eosin.Data ekspresi VEGF dianalisisdenganAnovafaktorial (general linear model) yang dilanjutkandenganujiTukey α= 0,05danhistopatologikulitsecaradeskriptif.Hasilpenelitianmenunjukkansalepekstra kkitosancangkangrajungan 5% sebagaiterapilukabakarpadatikustidakberbedanyata(p>0,05)dengangold standard(kombinasiekstrakplasentadan Neomycin sulfat)dandapatmenurunkanekspresi VEGF sebesar 79% padaharike 7 sertamampumemperbaikihistopatologikulitpada proses penyembuhanlukaharike 7berdasarkanre-epitelialisasi,pembentukanfolikelrambut, kelenjarsebaseadanjaringanikat.Kesimpulan dari penelitian ini adalah penggunaan salep kitosan 5% dapat digunakan untuk terapi topikal pada luka bakar
English Abstract
Burn is the trauma caused by fire, electric shock, chemicals, radiation and lightning that directly contacted skin, mucose or tissue. Chitosancontained in the crab shell (Portunus pelagicus) can be used as an alternative treatment of burn that potentially increases cell proliferation, triggers reepitalization, stimulates angiogenesys and antibacteria. This research aims to identify the effect of Chitosan Ointment that made from crab shell (Portunuspellagicus) toward burninducted rat towards vascullar endhotelial growth factor (VEGF)and skin histopathology . The research used 24 rats aged 2-3 months, and weight <150 g was analyzed by used Factorial Randomized Block Design (FRBD) three groups of block design: 3 therapies used chitosan 5%, 3 controls used placenta extract and neomycin sulfate for 7 days on topical twice a day. Then each group was observed on day 1, day 3, and day 7. The observation of VEGF expression using immunohistochemistry (IHK) staining by counting the number of expressed macrophage cells and histopathology skin with hematoxylin-eosin stain. Data were analyzed with statistical ANOVA Factorial (General linear model), continued by Tukey test with α = 0,05 and descriptive skin histopathology. The results showed that extract of chitosan crab shell (Portunuspelagicus)not significantly different (P>0,05) with gold standard(combination extract placenta and Neomycin sulfate), on day 7 could decrease VEGF expression up to 79% and repair histopathology based on reepitalization, hair follicle, sebaceous glands and connective tissue. In conclusion, thechitosan ointment can be used for topical therapy on burn condition
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/158/051810187 |
| Uncontrolled Keywords: | Luka bakar, HistopatologiKulit, VEGF, Kitosan, Cangkang rajungan.-Burn, Skin Histopathology, VEGF, Chitosan, Crab Shell |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.1 Injuries and wounds > 617.11 Burns and scalds |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 26 Feb 2019 03:51 |
| Last Modified: | 22 Oct 2021 03:45 |
| URI: | http://repository.ub.ac.id/id/eprint/161520 |
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