Siahaan, Davinci Oswald (2018) Pengaruh Ekstrak Etanol Daun Ubi Jalar Ungu (Ipomoea Batatas [L.] Lam) Terhadap Ekspresi Tnf-Α Dan Histopatologi Jejunum Tikus (Rattus Norvegicus) Model Inflammatory Bowel Disease Yang Diinduksi Indometasin. Sarjana thesis, Universitas Brawijaya.
Abstract
Inflammatory Bowel Disease (IBD) merupakan penyakit inflamasi pada saluran pencernaan. Inflammatory Bowel Disease (IBD) dapat disebabkan oleh efek samping indometasin. Indometasin secara oral dengan dosis 15mg/kg BB dapat menyebabkan inflamasi akut pada jejunum. Ekstrak daun ubi jalar ungu memiliki banyak antosianin yaitu jenis flavanoid yang bekerja sebagai antioksidan dan antinflamasi. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol daun ubi jalar ungu (Ipomoea batatas [L.] Lam) terhadap TNF-α dan histopatologi jejunum tikus (Rattus norvegicus) setelah mendapat paparan indometasin. Tikus (Rattus norvegicus) jantan strain Wistar berumur 8-12 minggu dengan berat 150- 200 gram dibagi menjadi 5 perlakuan, yaitu tikus kontrol positif, tikus kontrol negatif, tikus yang diinduksi indometasin yang diberi terapi ekstrak etanol daun ubi jalar ungu (Ipomoea batas [L.] Lam) dosis 600 mg/kg BB, dosis 700 mg/kg BB, dan dosis 800 mg/kg BB. Ekspresi TNF-α diamati dengan teknik imunohistokimia dan histopatologi jejunum tikus (Rattus norvegiccus) dilakukan dengan pewarnaan Hematoksilin dan Eosin. Data dianalisis menggunakan analisa kualitatif deskriptif dari gambaran histopatologi jejunum dan ekspresi TNF-α dianalisis menggunakan ragam ANOVA dan uji lanjutan BNJ (Beda Nyata Jujur). Hasil penelitian menunjukkan bahwa ekstrak etanol daun ubi jalar ungu (Ipomoea batatas [L.] Lam) secara signifikan (α=0,05) dapat menurunkan ekspresi TNF-α dan memperbaiki gambaran histopatologi jejunum pada tikus (Rattus norvegicus), dengan dosis terbaik 800 mg/kg BB. Kesimpulan dari penelitian ini adalah ekstrak etanol daun ubi jalar ungu (Ipomoea batatas [L.] Lam) dapat dijadikan terapi untuk IBD.
English Abstract
Inflammatory Bowel Disease (IBD) is an inflammatory disease of the digestive tract. Inflammatory Bowel Disease (IBD) is caused by indomethacin side effects. Indomethacin dose of 15 mg / kg BW which can cause acute inflammation of the jejunum. The Purple sweet potato leaf extract has many anthocyanins that are flavanoids that work as antioxidants and anti-inflammatories. This study was aimed to determine the effect of ethanol extract of purple sweet potato leaves (Ipomoea batatas [L.] Lam) to TNF-α and histopathology of rat’s jejunum (Rattus norvegicus) after receiving exposure to indomethacin. Rats (Rattus norvegicus) male Wistar strain aged 8-12 weeks weighing 150-200 grams divided into 5 grup, ie positive control rats, negative control rats, indomethacin induced rats treated by ethanol extract of purple sweet potato leaves (Ipomoea batatas [L.] Lam) dose 600 mg/kg BW, dose 700 mg/kg BW, and dose 800 mg/kg BW. TNF-α expression was observed by immunohistochemical and histopathologic techniques of rat’s jejunum (Rattus norvegiccus) performed with Hematoxylin and Eosin staining. Data were analyzed using descriptive quantitative analysis of jejunal histopathology and TNF- α expression were analyzed using ANOVA and Tukey’s HSD (honestly significant difference) test. The results showed that ethanol extract of purple sweet potato leaves (Ipomoea batatas [L.] Lam) significantly (α=0,05) decrease TNF-α expression and also repiring jejunum histopathology with the best dose 800 mg/kg BW. In conclussion extract ethanol purple sweet potato leaves (Ipomoea batatas [L.] Lam) that can be used as therapy for IBD
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/77/051807933 |
| Uncontrolled Keywords: | :Inflammatory Bowel Disease (IBD), Indometasin, daun ubi jalar ungu (Ipomoea batas [L.] Lam),Tumor necrosis faktor (TNF-α)-Inflammatory Bowel Disease (IBD), Indomethacin, purple sweet potato leaves (Ipomoea batatas [L.] Lam), Tumor necrosis factor (TNF-α) |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 94 Drugs derived from spesific plants (Convolvulaceae) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 21 Feb 2019 07:51 |
| Last Modified: | 21 Oct 2021 08:33 |
| URI: | http://repository.ub.ac.id/id/eprint/161491 |
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