Sari, Miranti Rachma Pratita (2018) Pengaruh Pemberian Terapi Ekstrak Serai (Cymbopogon citratus) Terhadap Kadar MDA (Malondialdehid) dan Gambaran Histopatologi Tubulus Seminiferus Tikus (Rattus norvegicus) yang Diinduksi Boraks. Sarjana thesis, Universitas Brawijaya.
Abstract
Boraks merupakan zat xenobiotik yang berbahaya bagi tubuh. Konsumsi boraks secara terus menerus dapat menyebabkan menyebabkan terakumulasinya boraks dibeberapa jaringan tubuh seperti otak, hati, lambung, serta organ reproduksi yaitu testis. sehingga efek pemberian boraks pada testis dapat menyebabkan atrofi testis. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian ekstrak serai (Cymbopogon citratus) yang mengandung antioksidan terhadap kadar malondialdehyde (MDA) dan diameter tubulus seminiferus pada tikus (Rattus norvegicus). Tikus putih (Rattus norvegicus) dibagi menjadi 5 kelompok perlakuan, yaitu kelompok kontrol negatif, kontrol positif yang dipapar boraks dengan dosis 260 mg/kg BB dan kelompok P1, P2, P3 yang mendapat terapi ekstrak serai dengan dosis berurut 15 mg/kg BB, 30 mg/kg BB, 60 mg/kg BB. Pemberian boraks dilakukan selama 14 hari per-oral menggunakan sonde. Terapi ekstrak serai dilakukan dengan cara per oral menggunakan sonde selama 14 hari. Pengukuran kadar MDA menggunakan metode TBA test dan diameter tubulus seminiferus menggunakan pewarnaan HE dan mikroskop mikrometer. Data kadar MDA dan diameter tubulus seminiferus dianalisa menggunakan analisis ragam one way ANOVA dengan taraf kepercayaan α=5%. Hasil penelitian menunjukkan bahwa terapi ekstrak serai pada tikus yang terpapar boraks secara signifikan (p<0,05) menurunkan kadar MDA sampai 27% dan meningkatkan diameter tubulus seminiferus sampai 57%. Kesimpulan dari penelitian ini adalah bahwa ekstrak serai mengandung antioksidan yang mampu menurunkan kadar MDA dan meningkatkan diameter tubulus seminiferus
English Abstract
Borax is a xenobiotic compound that dangerous for body. Borax consumption contonously can caused borax accumulation in some bodies tissue, such as: brain, liver, stomach and testes that become atrophy. This research was aimed to know effect of lemongrass (Cymbopogon citratus) extract that contain antioxidant towards MDA level and diameter of seminiferous tubules in rats (Rattus norvegicus). The rats (Rattus norvegicus) were divide into 5 groups: negatif control group (healthy rats), positive control group that induced by borax with dose of 260 mg/kg BW, and therapy group of extract lemongrass P1, P2, P3 with dose of 15 mg/kg BW, 30 mg/kg BW, and 60 mg/kg BW respectively. The administration of borax was performed for 14 days per-orally. Lemongrass (Cymbopogon citratus) extract administrator was given for 14 days per orally. The level of MDA measured by TBA test and diameter of seminiferous tubules using HE staining measured microscopically. MDA level and diameter of seminiferous tubules analyzed using one way ANOVA with α=5%. The result showed that the effect of lemongrass extract therapy on rats induced by borax significantly (p<0,05) decreased the MDA levels up to 27% and increased the diameter of seminiferous tubules up to 57%. It can be concluded that lemongrass extract contains antioxidant which is able to decrease MDA levels and increase the diameter of seminiferous tubules.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/83/051807939 |
| Uncontrolled Keywords: | Boraks , Ekstrak Serai, MDA, Antioksidan dan Diameter Tubulus Seminiferus-Borax, Lemongrass Extract, MDA Level, Antioxidant and Diameter Of Seminiferous Tubules |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.324 9 Drugs derived from spesific plant (Poales) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 26 Feb 2019 04:11 |
| Last Modified: | 21 Oct 2021 08:37 |
| URI: | http://repository.ub.ac.id/id/eprint/161487 |
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