Handayani, Novembrianti (2018) Perbandingan Salep Kitosan Ekstrak Cangkang Rajungan (Portunus pelagicus) Dan Obat Topikal Komersial Yang Di Induksi Luka Bakar Derajat II Dalam pada Tikus Putih (Rattus norvegicus) Terhadap Ekspresi TGF- β dan Jumlah Sel Makrofag di Kulit. Sarjana thesis, Universitas Brawijaya.
Abstract
Luka bakar adalah kerusakan jaringan permukaan tubuh disebabkan oleh panas pada suhu tinggi yang menimbulkan reaksi. Fase inflamasi merupakan fase awal penyembuhan luka, dimana makrofag yang berfungsi sebagai fagositosis jaringan mati. Makrofag akan menghasilkan TGF- β yang akan menginduksi proliferasi berbagai sel. Penelitian ini bertujuan untuk mengetahui perbandingan antara pemberian salep kitosan dan obat topical komersial (Neomycine sulfat dan ekstrak plasenta) terhadap peningkatan ekspresi TGF- β dan jumlah makrofag. Penelitian ini bersifat eksperimental membandingkan dua kelompok perlakuan (diinduksi luka bakar dengan pemberian obat topical komersial untuk pemeriksaan TGF-β dengan metode imunohistokimia dan kelompok terapi (diinduksi luka bakar dengan pemberian terapi salep kitosan ektrak cangkang rajungan konsentrasi 5% selama 1, 3, dan 7 hari). Perhitungan jumlah sel makrofag dengan cara pengamatan histopatologi kulit dan dianalisa secara deskriptif dengan pewarnaan Hematoxylin-Eosin dan ekspresi TGF-β dihitung menggunakan imunoratio dengan perbesaran mikroskop 400x. Analisa data dilakukan dengan uji t. Rata-rata ekspresi TGF-β pada hari ke-1, 3, 7 pada pemberian kitosan 5% yaitu 40.93, 41.29, 53.09 sedangkan pada obat topikal komersial yaitu 37.91, 40.37, 54.59. Rata-rata jumlah makrofag pada pemberian kitosan 5% yaitu 4.37, 8.10, 5.45 sedangkan pada obat topikal komersial yaitu 4.17, 6.92, 4.45. Hasil penelitian menunjukkan pemberian salep kitosan dari cangkang rajungan (Portunus pelagicus) tidak berbeda nyata dengan pemberian obat topical komersial terhadap peningkatan ekspresi TGF- β dan penurunan jumlah sel makrofag pada hari ke-1, 3 dan 7 (p>0.05). Kesimpulan dari penelitian ini salep kitosan dari ekstrak cangkang rajungan berpotensi dapat menyembuhkan luka bakar
English Abstract
Burns are damage to the body's surface tissue caused by heat at high temperatures which causes a reaction. The inflammatory phase is the initial phase of woud healing where there are macrophages that function as dead tissue phagocytosis. Macrophages will produce TGF- β which will induce proliferation of various cells. This study aims to determine the comparison between the administration of chitosan ointment and topical commercial medications (Neomycine sulfate and placental extract) to increase TGF-β expression and the number of macrophages. This study was experimental in comparing two treatment groups (induced burns with commercial topical medication administration (Neomycine sulfate and placental extract) for TGF-β examination with Immunohistochemical methods and two treatment groups (burns induced by therapy of concentrated chitosan extract ointment 5% for 1, 3 and 7 days). Calculation of the number of macrophage cells by histopathological observation of the skin and analyzed descriptively with Hematoxylin-Eosin staining and TGF- β expression were calculated using immunoratio with 400x microscope magnification. Data analysis is done by t test. The average expressions on days 1, 3, 7 on 5% chitosan administration were 40.93, 41.29, 53.09 while in commercial topical medication were 37.91, 40.37, 54.59. The average numbers of macrophages in 5% chitosan were 4.37, 8.10, 5.45 while in commercial topical medication are 4.17, 6.92, 4.45. The results showed that chitosan ointment from crab shell (Portunus pelagicus) was not significantly different from commercial topical medication administration (Neomycine sulfate and placenta extract) to increase TGF-β expression and decrease in the number of macrophage cells on days 1, 3 and 7 (p > 0.05). The conclusion of this study is that chitosan ointment from extracts of crab shells has the potential to heal burns
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/157/051810186 |
| Uncontrolled Keywords: | Cangkang rajungan, Kitosan, Luka bakar, sel makrofag, TGF-β.- Crab shells, Chitosan, Burns, Macrophage cells, TGF-β. |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.1 Injuries and wounds > 617.11 Burns and scalds |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 26 Feb 2019 03:30 |
| Last Modified: | 22 Oct 2021 03:44 |
| URI: | http://repository.ub.ac.id/id/eprint/161462 |
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