Septiana, Wahyu Intan (2018) Terapi Preventif Ekstrak Etanol Daun Teh Hijau (Camelia sinensis folium) Terhadap Jumlah Sel Radang dan TNF-α Pulmo Tikus (Rattus norvegicus) HasilPaparan Asap Rokok. Sarjana thesis, Universitas Brawijaya.
Abstract
Asap rokok merupakan salah satu radikal bebas yang dapat mengganggu kesehatan. Ketidakseimbangan antara radikal bebas dan jumlah antioksidan yang ada dalam tubuh akan mengakibatkan stress oksidatif. Radikalbebas yang tinggimenyebabkaninflamasidankerusakanpadaorganpulmo. Hal iniditandaiadanyasel-selradangdansalahsatusitokinproinflamasiTNF-α. Radikal bebas dapat dihambat dengan antioksidan eksogen yang terkandung didalam ekstrakteh hijau (Camellia sinensis). Penelitianinibertujuan untuk mengetahui pengaruh ekstrak ekstrak teh hijau terhadap penurunan jumlahselradangdanekspresi TNF-α pada organ pulmo. Penelitian ini menggunakan hewan coba tikus putih (Rattus novergicus) jantan berumur 8-12 minggu dengan berat badan rata-rata 250 gram yang dibagi menjadi 5 kelompok perlakuan yaitu kontrol negatif (tanpa pemberian asap rokok dan ekstrak teh hijau), kontrol positif (yang dipapar asap rokok sebanyak 3 batang/kelompok/hari selama 14 hari tanpa diberikan ekstrak teh hijau), dan kelompok perlakuan (yang diberi ekstrak teh hijau dengan dosis masing-masing kelompok 6,25 mg/250gBB;9,375 mg/250gBB;dan12,5mg/250gBB, danpemberian asap rokok). PerhitunganselradangpadahistopatologipulmosecaradeskriptifdanpemeriksaanTNF -αmenggunakanflowcitometri.Analisa kuantitatif TNF-α menggunakan uji Analysis of Variance (ANOVA) (α=0,05) dan dilanjutkan dengan uji Tukey test. Hasil pemberian preventif ekstrakteh hijausemakinbesardosis yang diberikanmakadapat menurunkan jumlah sel radangdanekspresiTNF-α yang lebihbesar. Kesimpulan penelitian ini adalah teh hijau (Camellia sinensis) mampumenurunkanjumlahselradangdanmenurunkan kadar TNF-α dengandosisterbaikyaitu9,375 mg/250gBB
English Abstract
Smoke is one of the free radicals that can interfere with health. The imbalance between free radicals and the amount of antioxidants present in the body can cause oxidative stress. High free radicals cause disorders of the pulmo organs and inflammation, which is maintained by inflammatory cells and proinflammatory cytokines TNF-α. Free radicals can be inhibited with exogenous antioxidants contained in green tea(Camellia sinensis)extract. This study was intended to determine the effect of green tea extractto decrease of inflammation cell and TNF-α expression in pulmo. This study used malerat (Rattusnovergicus) aged 8-12 weeks with body weight average 250 gram and divided into 5 groups of treatment those were negative control, positive control (that was white exposed to 3 cigarette/day for 14 days without green tea extract), and treatment group that was white given green tea extract (with each dose - group 25 mg/kgBB, 37,5 mg/kgBB, 50 mg/kgBB, then exposed to cigarette).Inflammation cell counts on pulmonary histopathology were descriptive and examination of TNF-α usedflowcitometry.The quantitative analysis of TNF-α used an Analysis of Variance (ANOVA) (α = 0.05) and followed with Tukey test. Prevention of the green teaextract reduced the number of inflammatory cells and is able to prevent pulsed histopathologic damage. TNF-α expression decreased significantly from the three doses of the green tea extract given. This reaserch showed that green tea(Camellia sinensis) extract prevent pulmohistopathologic damage, decrease the number of inflammatory cells, and decrease TNF-α level,with the best dose of9,375 mg/250 gBB
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/118/051808789 |
| Uncontrolled Keywords: | Antioksidan, Asap rokok, SelRadang, TehHijau, TNF-α-Antioxidants, Cigarette, Green Tea, Inflammatory Cells, TNF-α |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 624 Drugs derived from specific plants (Mangosteens, Tea) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 26 Feb 2019 07:16 |
| Last Modified: | 22 Oct 2021 02:38 |
| URI: | http://repository.ub.ac.id/id/eprint/161437 |
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