Silitonga, Evris Hikmat Immanuel (2018) Potensi Terapi Preventif Air Perasan Bawang Putih (Allium sativum) terhadap Aktivitas Protease dan Histopatologi illeum pada Tikus(Rattus norvegicus) Hasil induksi Rhodamin B. Sarjana thesis, Universitas Brawijaya.
Abstract
Rhodamin B merupakan zat warna sintetik yang dilarang digunakan dalam produk pangan, bawang putih mengandung antioksidan berupa alisin berfungsi menurunkan serta mencegah timbulnya radikal bebas. Penelitian bertujuan mengetahui pengaruh pemberian air perasan bawang putih (Allium sativum) terhadap aktivitas protease dan perubahan gambaran histopatologi illeum tikus (Rattus norvegicus) yang diberi rhodamin B melalui pakan. Penelitian menggunakan tikus jantan strain Wistar dibagi menjadi 5 kelompok yaitu kontrol negatif, kontrol positif diberi pakan mengandung rhodamin B dengan konsentrasi 600 ppm selama 14 hari, serta kelompok terapi preventif P1, P2, P3 diberikan air perasan bawang putih dengan volume pemberian 0,5mL/200gBB, 1,0mL/200gBB, 1,5mL/200gBB dengan konsentrasi air perasan bawang putih 25% setelah dipapar rhodamin B melalui pakan selama 14 hari. Aktivitas protease diukur dengan metode spektrofotometri dan histopatologi illeum diamati menggunakan pewarnaan HE. Data aktivitas protease dianalisa secara kuantitatif menggunakan one-way ANOVA, sedangkan histopatologi illeum dianalisa secara deskriptif. Hasil penelitian menunjukkan air perasan bawang putih memberikan pengaruh nyata (p<0,05) terhadap penurunan aktivitas enzim protease dan histopatologi illeum menunjukkan berkurangnya kerusakan sel epitel yang ruptur, infiltrasi sel radang, dan sel goblet yang hipertrofi. Air perasan bawang putih 0,5 ml/200gBB dengan konsentrasi air perasan bawang putih 25% merupakan volume efektif dalam menurunkan aktivitas protease sebesar 43% dan mencegah kerusakan gambaran histopatologi illeum tikus (Rattus norvegicus).
English Abstract
Rhodamin B is a prohibited synthetic dyestuff used in food products. Garlic contains an antioxidant of allicin and serves to reduce and prevent the occurrence of free radicals. The aimed of this research was to investigate the effect of garlic juice (Allium sativum) on the protease activity and the alteration of histopathologic image of rat’s illeum (Rattus norvegicus) induced by rhodamine B through feed. The experiment used male rat wistar strain divided into negative control group, positive control group which with rhodamine B with concentration of 600 ppm for 14 days, preventive therapy group P1, P2, P3 were given with garlic juice with a volume of 0.5mL/200gBW, 1.0mL/200gBW, and 1.5mL/200gBW, respective with 25% garlic juice concentration as well as exposure to rhodamine B through fed was prepare for 14 days. Protease activity was measured by spectrophotometry method and histopathology of illeum was observed microscopicaly use HE staining. Protease activity data were analyzed quantitatively using one-way ANOVA, while illeum histopathology was analyzed descriptively. The results showed that garlic juice had significant effect (p <0.05) on the decrease of protease enzyme activity and histopathology of illeum organ showed decrease of ruptured epithelial cell damage, inflammatory cell infiltration, and hypertrophy goblet cells. The volume of garlic juice of 0.5mL/200g body weight garlic juice with 25% garlic juice concentration was an effective volume as preventive in reducing protease activity which showed by 43% and preventing damage Rats illeum histopatology.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/149/051809110 |
| Uncontrolled Keywords: | Aktivitas protease, histopatologi illeum, air perasan bawang putih,rhodamin B.-Protease activity, illeum histopathology, garlic juice, rhodamine B. |
| Subjects: | 500 Natural sciences and mathematics > 571 Physiology and related subjects > 571.9 Diseases |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 08 Apr 2019 02:04 |
| Last Modified: | 22 Oct 2021 03:41 |
| URI: | http://repository.ub.ac.id/id/eprint/161425 |
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