Firoentina, Gabriela Hendra (2018) Pengaruh Pemberian Minyak Biji Kelor (Moringa oleifera) terhadap Ekspresi TGF-β (Transforming Growth Factor β ) dan Jumlah Sel Fibroblas pada Terapi Luka Insisi Hewan Model Tikus Putih (Rattus norvegicus). Sarjana thesis, Universitas Brawijaya.
Abstract
Luka insisi adalah rusaknya atau hilangnya kesatuan jaringan atau sebagian komponen jaringan karena teriris oleh benda tajam. Minyak biji kelor memiliki kandungan flavonoid yang berkemampuan sebagai anti-inflamasi, antioksidan dan antimikroba. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian minyak biji kelor (Moringa oleifera) terhadap peningkatan ekspresi TGF-β (Transforming Growth Factor β) dan jumlah sel fibroblas. Penelitian ini bersifat eksperimental dengan menggunakan Rancangan Acak Lengkap. Hewan model yang digunakan adalah tikus putih strain wistar jantan umur 8-12 minggu, berat badan 150-250 gram yang dibagi dalam 5 kelompok perlakuan yaitu kelompok kontrol negatif (sehat), kontrol positif, kelompok perlakuan terapi minyak biji kelor dengan konsentrasi bertingkat 50%, 75%, dan 100% pada daerah luka insisi. Terapi diberikan sehari dua kali selama 7 hari dan tikus dieuthanasi pada hari ke-8. Ekspresi TGF-β diamati dengan metode Imunohistokimia dan jumlah fibroblas diamati dengan pewarnaan Hematoksilin Eosin. Analisa data dilakukan secara kuantitatif dengan One Way ANOVA dan uji lanjutan Tukey (α=0,05). Hasil penelitian menunjukkan bahwa pemberian terapi minyak biji kelor secara signifikan dapat meningkatkan jumlah sel fibroblas dan ekspresi TGF-β dengan dosis efektif sebesar 75%. Kesimpulan dari penelitian ini yaitu pemberian minyak biji kelor dapat digunakan sebagai terapi alternatif untuk penyembuhan luka insisi
English Abstract
An incision wound is the breakdown or loss of a unified tissue or part of a tissue component due to a sharp cut. Moringa seed oil contained flavonoid which has the ability as anti-inflammatory, antioxidants and antimicrobials. The purpose of this study was to know about administration effect of Moringa oleifera oil to increase the expression of TGF-β (Transforming Growth Factor β) and the number of fibroblast cells. This research was an experimental research used Completely Randomized Design. Animal models that were used in this study consisted of male wistar rats aged 8-12 weeks old and weighed around 150-250 grams. Rats were divided into 5 treatment groups consisted of negative control group, positive control group, and treatment group of Moringa seed oil therapy with concentration of stratified 50%, 75%, and 100%. Treatment was administered twice daily for 7 days and the rats were euthanized on day 8. The expression of TGF-β was observed by Immunohistochemistry methods and the amount of fibroblasts were stained by Hematoxylin Eosin. Expression of TGF-β and the number of fibroblast were measured quantitatively and analyzed by One Way ANOVA and followed by Tukey test (α = 0,05). The results of this study showed that moringa seed oil therapy could significantly increase the number of fibroblast cells and TGF-β expression with an effective dosage of 75%. The conclusion of this study was moringa seed oil could be used as an alternative therapy of incision wound.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/135/051808855 |
| Uncontrolled Keywords: | Luka Insisi, Minyak Biji Kelor, TGF-β, Fibroblas-Incision wounds, Moringa seed oil, TGF-β, Fibroblast |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.1 Injuries and wounds |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 08 Apr 2019 01:47 |
| Last Modified: | 22 Oct 2021 03:30 |
| URI: | http://repository.ub.ac.id/id/eprint/161410 |
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