Khiftiyah, Siti Maria (2018) VCO (Virgin Coconut Oil) Hasil Pengasaman Jeruk Nipis sebagai Terapi Luka Insisi Hewan Model Infeksi Nosokomial Berdasarkan Kadar TGF β dan Ketebalan Epidermis. Sarjana thesis, Universitas Brawijaya.
Abstract
Luka pasca operasi dapat mengalami infeksi nosokomial yang memperlama kesembuhan luka. Infeksi nosokomial paling tinggi disebabkan oleh Staphylococcus aureus. Bakteri ini memiliki sifat multidrug resistant sehingga pemberian antibiotik dinilai kurang efisien. VCO (Virgin Coconut Oil) memiliki potensi untuk terapi luka insisi karena mengandung Medium Chain Trigliserida (MCT) terutama asam laurat yang bersifat antibakteri, mempercepat proliferasi fibroblast dan antiinflamasi. Penelitian ini bertujuan untuk mengetahui efek terapi VCO pada luka insisi hewan model nosokomial terhadap kadar TGF β dan ketebalan epidermis. Penelitian ini bersifat eksperimental dengan hewan coba mencit (Mus musculus) BALB/c jantan dengan berat 30 gram berumur 8 minggu yang dibagi menjadi 5 kelompok yaitu kontrol negatif (K-) adalah mencit yang dijahit lukanya dengan benang silk secara aseptis dan terapi antibiotic topikal, kontrol positif (K+) adalah mencit yang dijahit lukanya dengan benang dikontaminasi S.aureus tanpa pemberian terapi. Kelompok perlakuan 1, 2, dan 3 merupakan kelompok yang dijahit lukanya dengan benang yang dikontaminasi S.aureus dan diterapi VCO yaitu P1 (1x sehari), P2 (2x sehari), P3 (3x sehari). Volume pemberian VCO sebanyak 50µl diberikan selama 7 hari secara topikal. Perhitungan ketebalan epidermis diamati secara kuantitatif dengan pewarnaan HE. Kadar TGF β diukur secara kuantitatif menggunakan flowcytometry dan software BD cellquest ProTM. Semua data dianalisis menggunakan uji one way ANOVA dan uji lanjutan BNJ (Beda Nyata Jujur) dengan tingkat kepercayaan 95% (α = 0,05). Hasil penelitian menunjukkan terapi VCO 1x sehari menunjukkan peningkatan signifikan (p<0,05) terhadap kadar TGF β yaitu sebesar 3,76 ± 2,18 (% sel) jaringan kulit mencit model nosokomial. Pemberian terapi VCO frekuensi 1x sehari, 2x sehari, 3x sehari mampu meningkatkan ketebalan epidermis kulit mencit model nosokomial yaitu sebesar 5,64µm; 5,32µm; dan 5,00µm. Kesimpulan dari penelitian ini bahwa pemberian VCO memiliki potensi sebagai terapi alternatif luka infeksi nosokomial.
English Abstract
Nosocomial infection can prolong post-surgery wound healing. The highest nosocomial infection is caused by Staphylococcus aureus. These bacteria have multidrug resistant properties so that antibiotics are considered less efficient. VCO (Virgin Coconut Oil) has the potential to treat incision wounds because it contains lauric acid which can be antibacterial, accelerate proliferation of fibroblasts and anti-inflammatory. This study aimed to determine the effect of VCO therapy on nosocomial model animals based on the levels of TGF β and epidermal thickness. The experimental animals used male (Mus musculus) BALB / c mice weight of 30 grams, 8 weeks old which was divided into 5 groups, namely negative control (K- ) was sutured with silk thread aseptically and given topical antibiotic therapy, positive control (K+) was sutured with a thread contaminated S.aureus without therapy. Treatment groups 1, 2 and 3 were groups that sutured with threads contaminated S.aureus and treated with VCO namely P1 (1x daily), P2 (2x daily), P3 (3x daily). 50µl of VCO was performed over 7 days topically. Calculation of epidermal thickness was observed quantitatively with HE staining Levels of TGF β used flowcytometry and BD cellquest ProTM software. All data were analyzed by using one-way ANOVA test and BNJ with 95% confidence level (α = 0,05). The results showed VCO therapy 1x daily showed a significant increase (p <0.05) of TGF β levels in the amount of 3.76 ±2,18 (% cells) of skin tissue nosocomial mice models. The frequency of VCO therapy 1x daily, 2x daily, 3x daily was able to increase the epidermal thickness of the skin tissue of nosocomial mice models which was 5.64μm; 5,32μm; and 5,00μm. The conclusion of this study that VCO administration has the potential as an alternative therapeutic wound for nosocomial infections.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/128/051808848 |
| Uncontrolled Keywords: | Infeksi Nosokomial, Luka insisi, Virgin Coconut Oil, TGF β, Epidermis- Nosocomial Infection, wound, Virgin Coconut Oil, TGF β, Epidermal. |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.1 Injuries and wounds |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 08 Apr 2019 01:40 |
| Last Modified: | 22 Oct 2021 02:55 |
| URI: | http://repository.ub.ac.id/id/eprint/161405 |
Preview |
Text
Siti Maria Khiftiyah.pdf Download (3MB) | Preview |
Actions (login required)
 |
View Item |