Saputra, Tri (2018) Identifikasi Ekspresi Transforming Growth Factor (Tgf Β) Reseptor Dan Gambaran Histopatologi Ovarium Kasus Hipofungsi Ovarium Pada Kambing Peranakan Etawa (Pe). Sarjana thesis, Universitas Brawijaya.
Abstract
Kegagalan reproduksi atau kemajiran yang sering dialami ternak kambing adalah gagalnya fungsi hormonal reproduksi. Salah satu bentuk gangguan hormonal terhadap reproduksi hewan betina adalah hipofungsi ovarium yang ditandai dengan gangguan folikullogenesis. TGF β Receptor pada ovarium memiliki fungsi membantu proses follikulogenesis. Penelitian ini bertujuan membandingkan ekpresi resptor TGF β receptor dan gambaran histopatologi ovarium pada kambing yang mengalami hipofungsi dan kambing normal. Sampel ovarium yang digunakan sebanyak 10 sampel ovarium normal dan 10 sampel ovarium yang mengalami hipofungsi pada kambing PE yang berumur 8-10 bulan yang diperoleh dari Rumah Potong Hewan. Metode yang digunakan yaitu ovarium di cuci dengan NaCl kemudian dimasukan ke dalam pot yang berisi formalin 10%. TGF β receptor diamati dengan pewarnaan Imunohistokimia dan dianalisa dengan immunoratio. Hasil penelitian menunjukkan adanya ekspresi TGF β receptor pada folikel preantral dan antral pada kambing PE normal, sedangkan pada kambing PE yang mengalami hipofungsi terjadi penurunan ekspresi pada pada folikel preantral. Pada gambaran histopatologi ovarium kambing PE normal menunjukan perkembangan folikel preantral dan antral, sedangkan pada ovarium hipofungsi hanya terbentuk folikel preantral. Kesimpulan dari penilitian ini yaitu Ekspresi Transforming Growth Factor β (TGF β) reseptor pada ovarium normal memiliki persentase yang lebih tinggi dibandingkan dengan ovarium yang mengalami hipofungsi, pada pengamatan histopatologi folikel lebih baik perkembangannya pada ovarium normal dibandingkan ovarium hipofungsi
English Abstract
Reproductive failure or infertility of the goat is often experienced with failed hormonal reproductive function. One form of hormonal disturbances toward the animal's reproductive females is hypofunction ovary disorder marked by folliculogenesis. TGF β Receptor in the ovary has functioned to helps the process of folliculogenesis. This study aims to compare the expression TGF β receptor and ovarian histopathology description on goats from hypofunction group and normal group. The samples were 10 samples of normal ovaries and 10 samples from ovarian hypofunction in PE goats with 8-10 months. The samples were obtained from the Slaughterhouse Animals. Methods were the ovaries washed with NaCl then placed into a pot containing 10% formalin. TGF β receptor is observed by Immunohistochemical staining and analyzed by ImmunoRatio. The results showed the presence of TGF β receptors in preantral and antral follicles in normal PE goats, whereas in hypofunctional PE goats there was a decrease in preantral follicles. On normal PE goat histopathology picture shows the development of preantral and antral follicles, whereas in the ovary the hypofunction only produces preantral follicles. In the histopathologic ovaries, PE showed the development of preantral and antral follicles, whereas in the hipofunction ovary only preantral follicles were formed. The conclusion of this research is expression of Transforming Growth Factor β (TGF β) receptor in normal ovaries with higher percentage compared with hypofunctional ovaries, histopathological observation of follicles is better in normal ovaries than in hypofunction ovaries
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/120/051808840 |
| Uncontrolled Keywords: | Hipofungsi Ovarium, Folikullogenesis, Ekspresi Transforming Growth Factor β (TGF β) Receptor, Immunohistokimia, Hematoxylene Eosin.-Hypofunction ovary, Folliculogenesis, Transforming Growth Factor Receptor β (TGF β R), Immunohistochemistry, Hematoxylene Eosin |
| Subjects: | 500 Natural sciences and mathematics > 573 Specific physiological systems in animals, regional histology and physiology in animals > 573.6 Reproductive system |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 04 Apr 2019 02:14 |
| Last Modified: | 22 Oct 2021 02:40 |
| URI: | http://repository.ub.ac.id/id/eprint/161393 |
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