Ramadhan, Febry Sysdityawan (2018) Identifikasi Gen OVGP1 Pada Oviduk Kambing PE Dengan Kista Ovarium Menggunakan Metode Polymerase Chain Reaction (PCR). Sarjana thesis, Universitas Brawijaya.
Abstract
Kista ovarium merupakangangguan yang ditandai dengan adanya pertumbuhan sel-sel otot polos pada ovarium membentuk seperti balon yang berisi cairan. Oviductal Glycoprotein (OVGP1) disekresikan pada oviduk dan berperan dalam perkembangan embrio awal. Kejadian kista ovarium diduga dapat mempengaruhi tingkat ekspresi dari gen OVGP1.Tujuan dari penelitian ini adalah untuk mengetahui potensi gen OVGP1 sebagai biomarker untuk deteksi kista ovarium pada kambing dengan metode Polymerase Chain Reaction (PCR). Sampel yang digunakan yaitu DNA yang diisolasi dari oviduk kambing PE betina normal dan kambing dengan kista ovarium folikuler yang diperoleh dari RPH. Identifikasi gen OVGP1 dilakukan dengan metode PCR menggunakan primer forward (OVN1_F) 5’-GACTGCAACCCCTACAAGGA-3’ dan primer reverse (OVN1_R) 5’- GAGAGCAGCACCCACTTTTC-3’. Sekuensing hasil PCR dilakukan dengan metode Sanger. Analisa sekuen gen menggunakan program Bioedit dan NCBI BLAST dengan membandingkan sekuen hasil PCR pada kista ovarium folikuler dan normal ovarium dengan gen OVGP1 dari gene bank (NM_001285584.1). Hasil penelitian menunjukkan bahwa dua sampel normal dan dua sampel kista folikuler memiliki kemiripan diatas 90% dengan gen OVGP1 dari genebank NM_001285584.1. Sampel yang mengalami kista ovarium folikuler menunjukkan adanya perubahan susunan basa nukleotida berupa delesi, insersi, transisi dan transversi sehingga mempengaruhi susunan asam amino. Susunan asam amino pada sampel yang mengalami kista ovarium folikuler didapatkan adanya peningkatan jumlah proline (P), threonine (T) dan serine (S). Kesimpulannya terdapat perbedaan sekuen gen OVGP1 pada sampel kista ovarium folikuler dibandingkan dengan sampel ovarium normal. Sehingga gen OVGP1 bisa digunakan sebagai penanda kista ovarium folikuler
English Abstract
Ovarian cysts are a disorder characterized by the growth of smooth muscle cells in the ovaries forming like fluid-filled balloons. Oviductal Glycoprotein (OVGP1) is secreted in the oviduct and plays a role in early embryonic development. The incidence of ovarian cysts is thought to affect the level of expression of the OVGP1 gene. The purpose of this study was to determine the potancy of OVGP1 gene as biomarker for detection of ovarian cyst on goats by Polymerase Chain Reaction (PCR) method. The samples used were DNA isolated from normal female PE goat oviduct and goat with follicular ovarian cyst obtained from RPH. The OVGP1 gene identification was performed by PCR method using 5'- GACTGCAACCCACACAAGGA-3 forward primer (OVN1 _F) and reverse primer (OVN1_R) 5'-GAGAGCAGCACCCACTTTTC-3 '. The sequencing of PCR results is done by Sanger method. The gene sequence analysis used the Bioedit and NCBI BLAST programs by comparing the PCR sequence on the follicular ovarian cyst and the normal ovary with the OVGP1 gene of the gene bank NM_001285584.1. The results showed that two normal samples and two samples of follicular cysts had similarities above 90% with OVGP1 genebank NM_001285584.1. Samples with follicular ovarian cysts indicate a change in nucleotide base sequence due to deletions, insertions, transitions and transversions that affect the amino acid sequence. The arrangement of amino acids on samples with follicular ovarian cysts found an increase in the number of proline (P), threonine (T) and serine (S). In conclusion there is a difference in OVGP1 gene sequences in sample follicular ovarian cysts compared with sample normal ovaries . So the OVGP1 gene can be used as a marker of follicular ovarian cysts.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/95/051808356 |
| Uncontrolled Keywords: | Kambing Peranakan Etawa, kista ovarium, OVGP1, PCR-PE Goats, ovarian cysts, OVGP1, PCR |
| Subjects: | 500 Natural sciences and mathematics > 571 Physiology and related subjects > 571.9 Diseases > 571.91 Animal diseases |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 04 Apr 2019 01:39 |
| Last Modified: | 21 Oct 2021 08:50 |
| URI: | http://repository.ub.ac.id/id/eprint/161381 |
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