Fatimah, Ratu (2011) Brain-Formed Estrogen Regulates Motility through Dopamine Signaling in Early Development of Zebrafish (Danio rerio). Magister thesis, Universitas Brawijaya.
Abstract
It has been shown previously that estrogen (E 2 ) exposure to zebrafish embryos reduces expression of tyrosine hydroxylase in dopaminergic neurons and motility (response to tactile stimulation) of larvae. Moreover, expression of brain aromatase (Cyp19b) mRNA increases rapidly after 12 hour-post fertilization (hpf), which suggest brain-formed estrogen is needed in early development. In this study, aims of this research are to explore that estrogen reduces motility through dopamine signaling and investigate function of brain-formed estrogen in early development of zebrafish ( Danio rerio ). Zebrafish embryos were exposed to Sulpiride (50, 250 and 500μM) between 2 hpf (hour post-fertilization) and 72 hpf. Embryos were first exposed to 5 μM E 2 and 500 μM Sulpiride until 48 hpf followed by exposure of 50 μM L-DOPA until 72 hpf. Co-incubation 5 μM E 2 and 20 μM nomifensine was performed to verify mechanism of dopamine signaling. For second aim, aromatase enzyme was blocked by Fad (5, 10, 25 and 50 μM). Motility was performed by needlepoint stimulation at caudal region of quiescent zebrafish larvae. TH expression was analyzed by immunocytochemistry while expression of level Cyp19b and Cyp19a mRNA were measured by RT-PCR. results of this study shown that sulpiride exposure decreased motility in a dose dependent manner at 48 and 72 hpf as well as E 2 exposure. decreased motility by sulpiride and E 2 exposure was reversed by replacing with L-DOPA. Cyp19b mRNA and TH expression as well as motility were decreased after Fad exposure. conclusion from this study is indicated that endogenous estrogen produced in brain controlled by expression of brain aromatase regulates development of DA neurons during early development of zebrafish.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/639.374 82/FAT/b/041104215 |
| Subjects: | 600 Technology (Applied sciences) > 639 Hunting, fishing & conservation > 639.3 Culture of cold-blooded vertebrates |
| Divisions: | S2/S3 > Magister Biologi, Fakultas MIPA |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 23 Nov 2011 12:22 |
| Last Modified: | 23 Nov 2011 12:22 |
| URI: | http://repository.ub.ac.id/id/eprint/159068 |
Actions (login required)
 |
View Item |