Halim, AtikaMarisa (2017) Effects of Morinda citrifolia Leaves Hot-Water Extract on The Immunity of Giant Freshwater Prawn, Macrobrachium rosenbergii. Magister thesis, Universitas Brawijaya.
Abstract
Morinda Citrifolia, yang dikenal secara komersial karena Noni sering digunakan untuk meningkatkan kekebalan, senyawa fenolik yang kaya tumbuhan ini dengan sifat antioksidan. Tujuan dari penelitian ini adalah untuk menguji kinerja pertumbuhan, tingkat kelangsungan hidup, tanggapan imun bawaan, ekspresi gen imun dan resistensi terhadap lacococcus garvieae. Dalam penelitian ini, M. rosenbergii diberi makan dengan encer morinda citrifolia ekstrak daun (AMLE) -Cuplement pada 0,6, 4 dan 6 g KG-1. Total hemosit hitung (THC), aktivitas hemosit diferensial (DHC), aktivitas fenoloxidase (PO), aktivitas pernapasan bursts (rbs), superoksida dismutase (SOD), aktivitas glutathione peroxidase (GPX), aktivitas fagosit (PA), transglutaminase (TG) Kegiatan dan pembekuan waktu (CT) hemosit dilakukan untuk menilai respons imunologis sebelum dan setelah 7, 21, 35, 49 dan 63 hari pengobatan memberi makan. Persentase penambahan berat badan dan efisiensi makan serta dievaluasi setiap 15 hari setelah menyusui pengobatan IV. Ekspresi gen yang terkait imun termasuk propo, LGBP, peroksinektin, α2-makroglubulin, transglutaminase, crustin dan lisozim udang juga dilakukan dalam penelitian ini. Hasil penelitian menunjukkan bahwa setelah 60 hari perawatan pemberian makan, udang yang diberi makan dengan encer Morinda Citrifolia meninggalkan diet ekstrak di 0,6 g KG-1 telah meningkatkan peningkatan berat badan dan efisiensi pakan, dibandingkan dengan kelompok kontrol. THC dan DHC udang meningkat secara signifikan selama 63 hari perawatan pemberian makan, dan nilai tertinggi THC dan DHC diamati pada 0,6 g KG-1 dari encer M. Citrifolia meninggalkan diet ekstrak. PO, RBS, GPX, TG, dan PA udang yang diberi makan dengan 0,6 g KG-1 dari AMLE meningkat secara signifikan dan diketahui bahwa pada konsentrasi itu, respons imunologis udang meningkat secara signifikan, sedangkan kegiatan RBS dan GPX dikembalikan ke normal setelah 35 hari. Bulu Ritumum, CT udang menurun secara signifikan selama 63 hari pengobatan makan. Ekspresi gen seperti LGBP, propo, α2-makroglobulin, PE dan TG diregulasi ketika udang diberi makan dengan AMLE pada 0,6 g KG-1. Pada o r sisi peptida antimikroba (AMPS), seperti crustin dan lisozim meningkat secara signifikan setelah 3 hingga 63 hari pengobatan memberi makan. Fakta-fakta ini bukti dengan persentase tingkat kelangsungan hidup tertinggi dalam udang diamati pada 0,6 g KG-1 dari AMLE, dibandingkan dengan kelompok kelompok dan kontrol yang ditantang dengan L. Garvieae selama 144 jam. Studi Se menyimpulkan dan merekomendasikan bahwa diet ekstrak daun M. citrifolia berair pada 0,6 g kg-1 dapat digunakan sebagai zat imunostimulan dan tambahan untuk mempromosikan tingkat pertumbuhan, efisiensi pakan, meningkatkan respons imun dan ekspresi gen imun di M. Rosenbergii.
English Abstract
Morinda citrifolia, known commercially as noni is often used for boosting immunity, this plant-rich of phenolic compound with antioxidant properties. aim of this study was to examine growth performance, survival rate, innate immune responses, immune genes expressions and resistance against Lactococcus garvieae. In present study, M. rosenbergii fed with aqueous Morinda citrifolia leaves extract (AMLE)-supplemented diets at 0.6, 4 and 6 g kg-1. Total hemocyte count (THC), differential hemocyte count (DHC), phenoloxidase (PO) activity, respiratory bursts (RBs) activity, superoxide dismutase (SOD), glutathione peroxidase (GPx) activity, phagocytic activity (PA), transglutaminase (TG) activity and clotting time (CT) of hemocytes were conducted to assessed immunological responses before and after 7, 21, 35, 49 and 63 days of feeding treatment. Percentage of weight gain and feeding efficiency as well as evaluated every 15 days after of feeding iv treatment. Immune related genes expressions including proPO, LGBP, peroxinectin, α2-macroglubulin, transglutaminase, crustin and lysozyme of prawn were also carried out in this research. results showed that after 60 days of feeding treatments, prawns fed with aqueous Morinda citrifolia leaves extract-supplemented diets at 0.6 g kg-1 had increased significantly weight gain and feed efficiency, compared with control group. THC and DHC of prawns were increased significantly during 63 days of feeding treatments, and highest value of THC and DHC were observed at 0.6 g kg-1 of aqueous M. citrifolia leaves extract-supplemented diet. PO, RBs, GPx, TG, and PA of prawns fed with 0.6 g kg-1 of AMLE were significantly increased and it is known that at those concentration, immunological responses of prawns enhance significantly, whereas RBs and GPx activities of prawns return to normal after 35 days. Fur rmore, CT of prawn decreased significantly during 63 days of feeding treatment. gene expression such as LGBP, proPO, α2-macroglobulin, PE and TG was upregulated when prawn fed with AMLE at 0.6 g kg-1. On o r side antimicrobial peptides (AMPs), such as crustin and lysozyme increased significantly after 3 to 63 days of feeding treatment. This facts evidence by highest percentage of survival rates in prawn was observed at 0.6 g kg-1 of AMLE, compared with o r group and control group challenged with L. garvieae for 144 hours. se study concluded and recommended that dietary of aqueous M. citrifolia leaves extract at 0.6 g kg-1 can be used as a immunostimulant and additional substances to promotes growth rate, feed efficiency, enhance immune responses and immune genes expressions in M. rosenbergii.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/639.3/HAL/e/2017/041702820 |
| Subjects: | 600 Technology (Applied sciences) > 639 Hunting, fishing & conservation > 639.3 Culture of cold-blooded vertebrates |
| Divisions: | S2/S3 > Magister Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian |
| Depositing User: | Nur Cholis |
| Date Deposited: | 05 May 2017 14:48 |
| Last Modified: | 05 May 2017 14:48 |
| URI: | http://repository.ub.ac.id/id/eprint/159059 |
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