Phatama, KrisnaYuarno (2012) Efek Kayu Manis Indonesia pada Tikus Model Osteoporosis (Penelitian Eksperimental pada Tikus Model Osteoporosis Pascamenopause Akibat Ovariektomi). Magister thesis, Universitas Brawijaya.
Abstract
Penelitian ini untuk mengetahui efek ekstrak kayu manis Indonesia ( Cinnamomum burmannii ) pada stress oksidatif, petanda biokimia bone turnover , jumlah osteoblas dan osteoklas pada tikus osteoporosis pasca ovariektomi. Total 36 tikus Wistar betina secara acak dibagi menjadi 6 kelompok, yaitu 1 kelompok prosedur sham (A), 1 kelompok tikus ovariektomi (B), dan 4 kelompok tikus ovariektomi dengan penambahan ektrak kayu manis Indonesia (EKI) (C) (masing-masing 12,5; 25; 50; 100 mg/kg perhari selama 3 bulan). Prosedur ovariektomi dan analisa kadar malondialdehyde (MDA) dilakukan di Laboratorium Farmakologi. Ekspresi Osteocalcin (OC) dan c-telopeptide type I collagen (CTX) dianalisa dengan ELISA di Laboratorium Biomedik. Jumlah osteoblas dan osteoklas dari tulang tibia bagian atas dianalisa di Laboratorium Patologi Anatomi. Semua prosedur dilakukan di Fakultas Kedokteran Universitas Brawijaya, Malang. Kadar MDA, CTX, dan jumlah osteoklas meningkat secara signifikan pada kelompok B dibandingkan dengan A (p 0.05). Sedangkan kadar OC dan jumlah osteoblas kelompok B secara signifikan lebih rendah dibandingkan dengan kelompok A (p 0.05). Didapatkan penurunan kadar MDA di kelompok C secara signifikan dibandingkan dengan kelompok B (p 0.05). Kami dapatkan pula tidak adanya perubahan kadar CTX dan jumlah osteoklas pada kelompok C dengan dosis EKI lebih rendah (12,5 dan 25 mg/kg), tetapi terjadi peningkatan secara signifikan dengan dosis EKI lebih tinggi (50 dan 100 mg/kg). Kadar OC dan jumlah osteoblas kelompok C meningkat secara signifikan dibandingkan dengan kelompok B (p 0.05). EKI dosis lebih rendah bermanfaat sebagai penghambat osteoporosis dengan meningkatkan pembentukan tulang dan menurunkan stres oksidatif pada tikus osteoporosis pasca ovariektomi.
English Abstract
This research aim is to determine effect of Indonesian cinnamon (Cinnamomum burmannii) extract (ICE) on oxidative stress, serum bone turnover marker, number of osteoblast and osteoclast in osteoporosis induced-ovariectomized rats. A total of 36 Wistar female rats were randomly assigned into six groups including 1 sham operated group (A), 1 ovariectomized group (B), and 4 ovariectomized with ICE administration groups (C) (12.5; 25; 50; 100 mg/kg daily for 3 months, respectively). Ovariectomy procedure and analysis of malondialdehyde (MDA) level were done in Pharmacology Laboratory. expression of osteocalcin (OC) and c-telopeptide type I collagen (CTX) were analyzed using ELISA in Biomedical Laboratory. number of osteoblast and osteoclast of rat`s proximal tibia were analyzed in Pathology Anatomy Laboratory. All procedures were done at Medical Faculty, Brawijaya University, Malang. MDA level, CTX level and number of osteoclast were significantly higher in group B compared to group A (p 0.05). Contrary, OC level and number of osteoblast were significantly lower in group B compared to group A (p 0.05). re were decreasing MDA level in group C significantly compared to group B (p 0.05). We found no change of CTX level and number of osteoclast in group C with lower dose of ICE (12.5 and 25 mg/kg), but y increased significantly in higher dose of ICE (50 and 100 mg/kg). OC level and number of osteoblast increased significantly in group C compared to group B (p 0.05). Lower dose of ICE has beneficial effect as osteoporosis inhibitor by increasing bone formation and decreasing oxidative stress in osteoporosis induced-ovariectomized rats.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/633.83/PHA/e/041301350 |
| Subjects: | 600 Technology (Applied sciences) > 633 Field and plantation crops > 633.8 Other crops grown for industrial processing |
| Divisions: | Profesi Kedokteran > Spesialis Orthopedi dan Traumatologi, Fakultas Kedokteran |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 04 Apr 2013 11:50 |
| Last Modified: | 04 Apr 2013 11:50 |
| URI: | http://repository.ub.ac.id/id/eprint/158941 |
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