Pratiwi, FebyWijaya (2012) Immunosensor Based on Silver Enhancement of Magnetic-Latex-Biobarcode for Salmonella enterica serovar Typhimurium Detection. Magister thesis, Universitas Brawijaya.
Abstract
Uji yang sangat sensitif untuk S. Enterica Serovar Typhimurium berdasarkan label elektrokimia telah dikembangkan. Label adalah DNA biobarcode yang dimasukkan dengan peningkatan perak. Label dibangun dengan menggunakan polystyrene co-acrylic asam (PSA) inti, n dilapisi secara berurutan dengan manik magnetik yang dimodifikasi dan barcode DNA campuran dan antibodi monoklonal (disebut biobarcode magnetik-lateks). Cakupan DNA dan antibodi untuk 506 ± 21,5 nm diameter bola sekitar 3,51 × 10 5 dan 26 molekul masing-masing per bidang lateks. Pengukuran tipe sandwich dilakukan dengan mencampur antara antibodi polikkronisasi biotinylated, biobarcode magnetik-lateks, dan antigen pada 96 piring sumur. n, immuno-kompleks dipisahkan secara magnetis dari bahan yang tidak bereaksi (seperti biotinylated-pab). Biotinylated-immunocomplex secara langsung berinteraksi dengan layar karbon yang diimobilisasi Avidin. Ion perak secara elektrostatis terkait dengan barcode DNA probe negatif, diikuti dengan pengurangan autokatalitik ke logam perak. Konten perak dari label biobarcode magnetik-lateks adalah n ditentukan oleh voltametri pengupasan anodik setelah oksidasi logam perak hingga ion perak. S. Enterica Serovar Typhimurium diukur dengan batas deteksi sekitar 12 CFU ML -1. Immunoassay dapat mendeteksi S. Enterica Serovar Typhimurium dalam sampel makanan.
English Abstract
A highly sensitive assay for S. enterica serovar Typhimurium based on electrochemical label has been developed. label is DNA biobarcode incorporated with silver enhancement. label was constructed by using polystyrene co-acrylic acid (PSA) core, n coated sequentially with APTS modified magnetic bead and mixed DNA barcode and monoclonal antibody (called magnetic-latex biobarcode). DNA and antibody coverage for 506 ± 21.5 nm diameter spheres was approximately 3.51 × 10 5 and 26 molecules per latex spheres, respectively. sandwich-type measurement was performed by mixing between biotinylated-polyclonal antibody, magnetic-latex biobarcode, and antigen in 96 well plates. n, immuno-complex was magnetically separated from unreacted materials (such as biotinylated-pAb). biotinylated-immunocomplex directly interacted with avidin immobilized carbon screen printed electrode. Silver ions were n electrostatically associated with negative probe DNA barcode, followed by autocatalytic reduction to silver metal. silver content of magnetic-latex biobarcode label was n determined by anodic stripping voltammetry after oxidation of silver metal to silver ions. S. enterica serovar Typhimurium was measured with a limit of detection of about 12 CFU mL -1 . immunoassay was able to detect S. enterica serovar Typhimurium in food samples.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/621.399 4/PRA/i/041201140 |
| Subjects: | 600 Technology (Applied sciences) > 621 Applied physics > 621.3 Electrical, magnetic, optical, communications, computer engineering; electronics, lighting |
| Divisions: | S2/S3 > Magister Biologi, Fakultas MIPA |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 17 Dec 2013 11:54 |
| Last Modified: | 17 Dec 2013 11:54 |
| URI: | http://repository.ub.ac.id/id/eprint/158703 |
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