Yuliarto, Saptadi (2010) Hubungan Ekspresi Interleukin (IL)-4 dengan Bax dan Apoptosis Limfosit pada Bronkiolus dan Paru Mencit Asma. Magister thesis, Universitas Brawijaya.
Abstract
Penurunan apoptosis limfosit merupakan salah satu faktor penyebab keradangan kronis saluran nafas pada asma alergik. Beberapa penelitian menunjukkan peran interleukin (IL)-4 dalam penghambatan apoptosis limfosit, namun masih sedikit penelitian tentang peran Bax-limfosit dan hubungannya dengan IL-4 pada asma. Tujuan penelitian ini adalah membuktikan peran IL-4 dalam menghambat apoptosis limfosit melalui penurunan Bax-limfosit pada bronkiolus dan paru mencit asma. Penelitian ini merupakan penelitian eksperimental dengan metode randomized control group . Subyek penelitian adalah mencit Balb/c yang dibagi dalam 2 kelompok: asma dan non-asma. Kelompok asma disensitisasi dengan ovalbumin secara intraperitoneal pada hari ke-0 dan 14, diikuti dengan cara inhalasi setiap 2-3 hari selama 6 minggu. Pada minggu ke-8, seluruh subyek diterminasi. Ekspresi IL-4 dan Bax-limfosit diperiksa dengan metode imunohistokimia, sedangkan apoptosis limfosit dengan metode TUNEL. Analisis statistik menggunakan independent sample t-test , Mann-Whitney U test, analisis regresi, dan analisis jalur dengan interval kepercayaan 95%. Spesimen bronkiolus dan paru didapatkan dari 18 subyek (9 dari masing-masing kelompok). Jumlah apoptosis limfosit sama antara kedua kelompok ( p =0,116), sedangkan Bax-limfosit menurun pada kelompok asma ( p =0,003). Hal ini menunjukkan penurunan apoptosis limfosit pada kelompok asma. Sebaliknya, ekspresi IL-4 meningkat pada kelompok asma ( p =0,00). Terdapat hubungan negatif sedang ( r 2 =0,29, p =0,011) antara IL-4 dan apoptosis limfosit. Terdapat pula hubungan negatif kuat ( r 2 =0,39, p =0,002) antara IL-4 dan ekspresi Baxlimfosit. Namun, tidak ditemukan hubungan antara Bax-limfosit dan apoptosis limfosit ( r =0,37, p =0,065). Analisis jalur menunjukkan pengaruh IL-4 terhadap apoptosis limfosit secara langsung lebih besar dibandingkan pengaruh tidak langsung melalui penurunan Bax limfosit. Sebagai kesimpulan, peningkatan ekspresi IL-4 menghambat apoptosis limfosit, namun bukan melalui penurunan Bax-limfosit.
English Abstract
Decrease of lymphocyte apoptosis is one factor that leads to chronic airway inflammation in allergic asthma. Previous studies revealed role of interleukin (IL)-4 in preventing lymphocyte apoptosis; however re are few studies about role of lymphocyte-Bax and its relationship with IL-4 in asthma. aim of study is to demonstrate IL-4 role in preventing lymphocyte apoptosis via lymphocyte-Bax inactivation in bronchioli and lungs of asthmatic mice. It is a randomized control group experimental study. subject was Balb/c mice that categorized into 2 groups: asthma and non-asthma. Asthma group was sensitized by ovalbumin intraperitoneally in day 0 and 14, followed by inhalation every 2-3 days for 6 weeks. In week 8, all of mice were terminated. IL-4 and lymphocyte-Bax expression was examined through immunohistochemistry method, whereas lymphocyte apoptosis by TUNEL method. Independent sample t-test, Mann Whitney U test, regression analysis, and path analysis was used in statistical analysis with confident interval 95%. bronchioli and lungs specimens were obtained from 18 mice (9 in each group). Lymphocyte apoptosis was similar between 2 groups ( p =0.116), additionally lymphocyte-Bax decreased in asthmatic group ( p =0.003). This indicated low activity of lymphocyte apoptosis in asthmatic group. Conversely, IL-4 expression increased in asthmatic group ( p =0.00). re was moderate negative correlation ( r 2 =0.29, p =0.011) between IL-4 and lymphocyte apoptosis. re was also strong negative correlation ( r 2 =0.39, p =0.002) between IL-4 and lymphocyte-Bax expression. However, we did not find any correlation between lymphocyte-Bax and lymphocyte apoptosis ( r =0.37, p =0.065). Path analysis revealed stronger direct relationship between IL-4 and lymphocyte apoptosis ra r than indirect relationship via lymphocyte-Bax inactivation. We conclude that increases of IL-4 expressions inhibit lymphocyte apoptosis; however, it is not due to Bax-lymphocyte inactivation.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/618.922 38/YUL/h/041002499 |
| Subjects: | 600 Technology (Applied sciences) > 618 Gynecology, obstetrics, pediatrics, geriatrics > 618.9 Pediatrics and geriatrics |
| Divisions: | Profesi Kedokteran > Spesialis THT Kepala dan Leher, Fakultas Kedokteran |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 28 Aug 2012 15:50 |
| Last Modified: | 28 Aug 2012 15:50 |
| URI: | http://repository.ub.ac.id/id/eprint/158578 |
Actions (login required)
 |
View Item |