Khairiah, Rahayu (2014) Pengaruh Genistein Terhadap Ekspresi Reseptor Estrogen α & β Pada Kultur Sel Endometriosis. Magister thesis, Universitas Brawijaya.
Abstract
Tujuan: Penelitian ini bertujuan untuk membuktikan pengaruh pemberian genistein terhadap ekspresi Reseptor Estrogen α (RE-α) dan Reseptor Estrogen β (RE-β) pada kultur sel endometriosis. Metode: Desain penelitian ini menggunakan studi eksperimental, yang dilakukan di Laboratorium Fisiologi Fakultas Kedokteran Universitas Brawijaya Malang. Menggunakan jaringan endometriosis yang diambil dari pasien endometriosis melalui laparoskopi, kemudian dilakukan kultur sel hingga konfluen. Kelompok kultur dibagi menjadi 7 kelompok, yaitu : kontrol, kultur + genistein 5μM/L, kultur + genistein 10μM/L, kultur + genistein 20μM/L, kultur + genistein 30μM/L, kultur + genistein 40μM/L, kultur + genistein 50μM/L dengan waktu inkubasi 6 jam, 24 jam dan 48 jam. Ekpresi RE-α & RE-β diperiksa dengan menggunakan flowcytometri. Data hasil pengamatan di analisis dengan uji ANOVA dan uji regresi. Hasil: Terdapat perbedaan yang bermakna (p 0,05) ekspresi RE-α dan RE-β antara kelompok kontrol dengan kelompok perlakuan. Pemberian genistein dosis 50μM/L menunjukkan nilai rerata ekspresi RE-α (2.15±1.42 %) & RE-β (4.00±3.54 % ) terendah dibandingkan dosis lain. Waktu inkubasi 24 jam dapat menurunkan ekspresi RE-α (0.85±0.39 %) & RE-β (1.78±0.6 %) paling rendah. Kesimpulan: Ada pengaruh pemberian genistein terhadap ekspresi RE-α dan RE-β pada kultur sel endometriosis.
English Abstract
Objective: To demonstrate effect of genistein on estrogen α receptor (ER-α) and estrogen β receptor (ER-β) expression in endometriosis cell culture. Methode: design of this study was experimental, study had been performed in Physiology Laboratory Medical Faculty of Brawijaya University Malang. This study used endometriosis tissue from patients with endometriosis via laparoscopy, cultured to make aconfluent.Culture group was divided into 7 groups: control, culture + genistein 5μM / L, culture + genistein 10μM/L, culture + genistein 20μM/L, culture + genistein 30μM/L, culture + genistein 40μM/L, culture + genistein 50μM/L with an incubation time of 6 hours, 24 hours and 48 hours. Estrogen α receptor &Estrogen β reseptor expression was determined by examination flowcytometri. data were analyzed with ANOVA and regression test. Result: re was significant difference (p 0.05) ER-α and ER-β expression control group compared with treatment group Dose of genistein 50μM/L indicated mean ER-α(2.15±1.42 %) & ER-β (4.00±3.54%)Expression in lowest dose when compared with o r treatment dose. 24- hour incubation time was decrease lowest ER-α(0.85±0.39 %)& ER-β (1.78±0.6 %) expression. Conclusion: re is significant of genistein on ER-α& ER-β expression in endometriosis cell culture.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/618.142/KHA/p/041401534 |
| Subjects: | 600 Technology (Applied sciences) > 618 Gynecology, obstetrics, pediatrics, geriatrics > 618.1 Gynecology |
| Divisions: | S2/S3 > Magister Kebidanan, Fakultas Kedokteran |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 03 Apr 2014 12:03 |
| Last Modified: | 03 Apr 2014 12:03 |
| URI: | http://repository.ub.ac.id/id/eprint/158550 |
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