Herawati, Ita (2014) Pemberian Genistein Berbagai Dosis Dan Waktu Inkubasi Terhadap Penurunan Kadar TNF-α Dan IL-6 Pada Kultur Jaringan Endometriosis. Magister thesis, Universitas Brawijaya.
Abstract
Tujuan : Mengetahui pengaruh genistein terhadap penurunan kadar TNF-α dan IL-6 pada kultur jaringan endometriosis. Metode : Penelitian ini dilakukaan di Laboratorium Fisiologi Fakultas Kedokteran Universitas Brawijaya Malang dengan menggunakan kultur jaringan endometriosis yang dipapar genistein berbagai dosis dan waktu. Jaringan endometriosis diambil melalui laparoskopi. Penelitian ini menggunakan desain eksperimental, Posttest only with control group, yang dibagi menjadi 7 kelompok yaitu kelompok kontrol, kelompok dosis 5 µM/L kelompok dosis 10 µM/L, kelompok dosis 20 µM/L, kelompok dosis 30 µM/L, kelompok dosis 40 µM/L dan kelompok dosis 50 µM/L dengan masa inkubasi 6 jam, 24 jam dan 48 jam. Pengukuran kadar TNF-α dan IL-6 menggunakan ELISA. Hasil : Hasil uji Anova menunjukan genistein secara signifikan menurunkan kadar TNF-α dan IL-6 (p 0,000), rerata terendah kadar TNF-α dan IL-6 pada dosis 50 µM/L dengan waktu inkubasi 6 jam. Kesimpulan : Pemberian genistein menurunkan kadar TNF-α dan IL-6 pada kultur sel endometriosis
English Abstract
Objective : to determine effect of genistein on TNF - α and IL – 6 levels in tissue culture endometriosis. Methods : This research had been conducted in Laboratory of Physiology Faculty of Medicine, University of Brawijaya using tissue culture endometriosis were exposed to genistein with various doses and time. endometriosis tissue taken via laparoscopy . This study used an experimental design posttest only with control group, which were divided into 7 groups: control group , group a dose of 5 µM/L, 10 µM/L, 20 µM/L, 30 µM/L, 40 µM/L and 50 µM/L and incubate in 6 hour, 24 hour and 48 hour. Measurement of levels of TNF - α and IL - 6 using ELISA . Result : Analizyng with Anova showed genistein significantly decreased levels of TNF - α and IL - 6 ( p 0.000 ) , lower mean levels of TNF – α and IL - 6 at are at dose of 50 lm / L with a 6 hour incubation period. Conclutions: Genistein Decrease TNF-α and IL-6 Levels In Endometriosis Cell Culture
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/618.142/HER/p/041401546 |
| Subjects: | 600 Technology (Applied sciences) > 618 Gynecology, obstetrics, pediatrics, geriatrics > 618.1 Gynecology |
| Divisions: | S2/S3 > Magister Kebidanan, Fakultas Kedokteran |
| Depositing User: | Hasbi |
| Date Deposited: | 03 Apr 2014 11:41 |
| Last Modified: | 03 Apr 2014 11:41 |
| URI: | http://repository.ub.ac.id/id/eprint/158548 |
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