Nuransyah, Andy (2013) Pengaruh Eritropoetin Terhadap Pembentukan Seroma Pasca Mastektomi Pada Kelinci Studi Eksperimental Laboratoris. Magister thesis, Universitas Brawijaya.
Abstract
Latar Belakang : Insiden terjadinya seroma pasca mastektomi sangat tinggi, bermacam cara pencegahan dilakukan namun kurang efektif disebabkan karena patofisiologinya yang belum jelas. Saat ini diketahui bahwa seroma merupakan eksudat akibat pemanjangan proses inflamasi. Penelitian ini ingin melihat pengaruh pemberian Eritropoetin (EPO) terhadap pembentukan seroma pasca mastektomi pada kelinci. Bahan dan metode : dilakukan penelitian terhadap 20 ekor kelinci yang dilakukan mastektomi, dibagi menjadi dua kelompok ; kelompok EPO diberikan EPO 1000 iu/kgbb sc dan kelompok kontrol tanpa pemberian EPO. Hari ketujuh dilakukan pengukuran jumlah seroma, kadar TNF-α serum dan seroma serta jumlah infiltrasi sel makrofag pada jaringan flap. Hasil : Didapatkan jumlah seroma kelompok EPO (0,47 ± 0,46 ml), lebih kecil daripada kontrol (3,09 ± 1.7 ml) dengan perbedaan yang signifikan (p=0,001). Kadar TNF-α serum kelompok EPO lebih rendah daripada kelompok kontrol (EPO 14,93 ± 1,24 ng/L, Kontrol 15,42 ± 1,10, p=0,376). Kadar TNF-α seroma kelompok EPO lebih tinggi daripada kelompok Kontrol (EPO14,67 ± 0,92 ng/L, Kontrol 10,93 ± 3,6 ng/L, p=0.045). Dan jumlah infiltrasi sel makrofag kelompok EPO lebih kecil daripada kelompok Kontrol (EPO 1,51 ± 0,61 sel/lpb, Kontrol 7,05 ± 0,86 sel/lpb, p=0,00) Kesimpulan : Pemberian EPO pada kelinci post mastektomi menurunkan volume seroma dan menurunkan jumlah infiltrasi sel makrofag yang menggambarkan proses inflamasi yang lebih cepat. Pada penelitian ini tidak dapat menerangkan peran TNF-α akibat EPO pada proses pembentukan seroma.
English Abstract
Background : incidence of seroma after mastectomy is high, but prevention managements were less effective due to pathophysiology was stll unclear. Currently it is known that seroma was exudates due to elongation of inflammatory process. This study intent to see effect of erythropoietin ( EPO ) on post-mastectomy seroma formation in rabbits . Materials and methods : examined of 20 rabbits were performed mastectomy , divided into two groups: EPO group administered EPO 1000 iu / kg sc and a control group without administration of EPO . Measured amount of seroma , serum & seroma levels of TNF - α and number of infiltrating macrophages in flap tissue on day seventh . Result: Seroma volume in EPO group (0.47 ± 0.46 ml) , smaller than controls (3.09 ± 1.7 ml ) with a significant difference (p = 0.001) . TNF - α levels in serum EPO group is lower than control group ( EPO 14.93 ± 1.24 ng / L , control 15.42 ± 1.10 , p = 0.376 ) . Levels of TNF - α seroma in EPO group was higher than control group ( EPO 14 , 67 ± 0.92 ng / L , control 10.93 ± 3.6 ng / L , p = 0.045 ) . And number of infiltrating macrophages in EPO group is smaller than control group ( 1.51 ± 0.61 EPO cells / LPB , control 7.05 ± 0.86 cells / LPB , p = 0.00 ) Conclusion : Administrated of EPO in post mastectomy rabbits make lowered seroma volume and decrease number of infiltrating macrophages that describe inflammatory process was faster than control group . In this study could not explain role of TNF - α due to EPO in formation of seroma
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/616.994.490.59/NUR/p/041402144 |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease |
| Divisions: | Profesi Kedokteran > Spesialis Ilmu Bedah, Fakultas Kedokteran |
| Depositing User: | Hasbi |
| Date Deposited: | 15 Apr 2014 14:35 |
| Last Modified: | 15 Apr 2014 14:35 |
| URI: | http://repository.ub.ac.id/id/eprint/158479 |
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