Siagian, LolyRotuaD (2010) Pengaruh Tumor Nekrosis Faktor α dan Malaria dalam Patogenesis Anemia pada Malaria Plasmodium falciparum in vitro. Magister thesis, Universitas Brawijaya.
Abstract
Anemia masih merupakan penyebab komplikasi tertinggi akibat infeksi Plasmodium falciparum. Saat ini penyebab anemia masih banyak diperdebatkan. Selain karena adanya lisis eritrosit berparasit dan terjadinya diseritropoisis juga diduga berperan faktor imunologi. Penelitian sebelumnya ditemukan terjadinya anemia setelah terapi walau parasit telah menghilang dari dalam darah, eritrofagositosis pada darah perifer dan DAT ( Direct Antiglobulin Test ) positif berhubungan dengan anemia. Adanya hubungan antara TNF a dengan eritrofagositosis semakin menguatkan terlibatnya faktor imunologi dalam patogenesa terjadinya anemia pada malaria P. falciparum . Penelitian ini bertujuan untuk mengetahui adanya pengaruh malaria dan penambahan TNF a dalam meningkatkan aktifitas fagosit makrofag terhadap eritrosit terinfeksi Plasmodium falciparum pada kultur monosit-makrofag darah manusia. Kultur monosit darah yang berasal dari penderita malaria dan donor normal (dibagai dalam 8 perlakuan) diaktivasi dengan pemberian TNF a 16 pg/ml, kemudian dipapar dengan eritrosit terinfeksi parasit Plasmodium falciparum (kultur P. falciparum ) dan eritrosit normal. Dinilai parameter rerata persentase jumlah sel makrofag dalam keadaan normal, aktivasi, apoptosis, nekrosis, fagositik (eritrofagositosis) dan rerata indeks fagosit. Pada kultur makrofag malaria, malaria dan penambahan TNF a berturut-turut menunjukkan perbedaan yang bermakna dengan kultur makrofag normal untuk rerata persentase jumlah sel makrofag aktivasi (Makrofag Normal + Eritrosit Normal = 19,93%; Makrofag Malaria + TNF a 16 pg/ml + Eritrosit terinfeksi P. falciparum = 66,73% dengan p= 0,000); rerata persentase jumlah sel makrofag nekrosis (Makrofag Normal + Eritrosit Normal = 1,77%; Makrofag Malaria + TNF a 16 pg/ml + Eritrosit terinfeksi P.falciparum = 30,73% dengan p= 0,000) dan rerata persentase jumlah sel makrofag fagosit {eritrofagositosis} (Makrofag Normal + Eritrosit Normal = 36,12%; Makrofag Malaria + TNF a 16 pg/ml + Eritrosit terinfeksi P. falciparum =11,66% dengan p= 0,031). Sedangkan untuk rerata persentase jumlah sel makrofag apoptosis (Makrofag Normal + Eritrosit Normal = 0,70%; Makrofag Malaria + TNF a 16 pg/ml + Eritrosit terinfeksi P. falciparum = 0,80% dengan p= 1,000) dan rerata indeks fagosit (Makrofag Normal + Eritrosit Normal = 1.09; Makrofag Malaria + TNF a 16 pg/ml + Eritrosit terinfeksi P. falciparum = 1,40 dengan p= 0,994) tidak menunjukkan perbedaan yang bermakna. Dapat disimpulkan bahwa malaria dan penambahan TNF a meningkatkan aktifitas fagosit makrofag terhadap eritrosit terinfeksi Plasmodium falciparum yang berperan dalam patogenesis anemia pada malaria P. falciparum .
English Abstract
Anaemia is still represent highest cause of complication in Plasmodium falciparum infection. In this time etiology of anaemia is still a lot of disputed. Besides caused by lysis parasitic erythrocyte and dyserythropoeisis, anaemia is also caused by immunologic factors. In previous research were found anaemia after treatment although parasite have disappeared from blood, erythrophagocytosis in peripheral blood and positive DAT (Direct Antiglobulin Test) related to anaemia. Relationship between TNF a and erythrophagocytosis streng n immunologic factors in pathogenesis of anaemia in malaria Plasmodium falciparum. aim of this research, to know influence of TNF a in increasing macrophage phagocyte activity to Plasmodium falciparum-infected erythrocytes on human blood monocyte-macrophage culture. Culture monocyte from normal blood and malaria patient (divided in 8 treatments) activated by TNF a 16 pg/ml, exposed to Plasmodium falciparum-infected erytrocytes (culture P. falciparum) and normal erytrocytes. average of percentage normal macrophages, activated macrophages, apoptosis macrophages, necrosis macrophages, phagocytic macrophages (erythrophagocytosis) and average of index phagocyte were evaluated. In malaria macrophages culture, malaria and addition of TNF a showed significant difference with normal macrophages culture at average percentage activated macrophages (Normal Macrophage + Normal Erythrocytes = 19,93%; Malaria Macrophage + TNF a 16 pg/ml + P. falciparum- infected erythrocytes = 66,73% with p= 0,000); at average percentage macrophages necrosis (Normal Macrophage + Normal Erythrocytes = 1,77%; Malaria Macrophage + TNF a 16 pg/ml + P. falciparum- infected erythrocytes =30,73% with p= 0,000) and at average percentage phagocytic macrophage {erythrophagocytosis} (Normal Macrophage + Normal Erythrocytes = 36,12%; Malaria Macrophage + TNF a 16 pg/ml + P. falciparum- infected erythrocytes = 11,66% with p= 0,031), respectively. But for average percentage apoptosis macrophages (Normal Macrophage + Normal Erythrocytes = 0,70%; Malaria Macrophage + TNF a 16 pg/ml + P. falciparum- infected erythrocytes = 0,80% with p= 1,000) and average index phagocyte (Normal Macrophage + Normal Erythrocytes = 1,09; Malaria Macrophage + TNF a 16 pg/ml + P. falciparum- infected erythrocytes = 1,40 with p= 0,994) did not show significant difference.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/616.936 2/SIA/p/041100261 |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease |
| Divisions: | Profesi Kedokteran > Spesialis THT Kepala dan Leher, Fakultas Kedokteran |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 23 Apr 2012 13:53 |
| Last Modified: | 23 Apr 2012 13:53 |
| URI: | http://repository.ub.ac.id/id/eprint/158457 |
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