Rosyidah, A`liyatur (2013) Studi Mekanisme Daun Tempuyung (Sonchus arvensis) dalam Menurunkan Akumulasi Kalsium Oksalat pada Mencit (Mus musculus). Magister thesis, Universitas Brawijaya.
Abstract
Akumulasi kalsium oksalat merupakan salah satu faktor utama penyebab batu ginjal (nefrolithiasis). Adanya oksalat dalam ginjal dapat menyebabkan peningkatan radikal bebas melalui reaksi peroksidasi lipid serta menyebabkan terjadinya nekrosis. Daun tempuyung ( S. arvensis ) telah lama digunakan sebagai obat tradisional untuk meluruhkan batu ginjal. Namun perannya dalam meluruhkan akumulasi kalsium oksalat sampai sekarang masih belum diketahui. Penelitian ini bertujuan untuk mengetahui mekanisme daun tempuyung ( S. arvensis ) dalam menurunkan akumulasi kalsium oksalat ginjal, ekspresi MDA ginjal, radikal bebas plasma, kreatinin darah, dan nekrosis sel ginjal mencit ( M. musculus ) yang dinduksi akumulasi kalsium oksalat. Penelitian ini menggunakan hewan coba mencit ( M. musculus) galur Swiss dengan berat rata-rata 25-30 g. Hewan coba dibagi dalam lima kelompok yaitu Normal; Akumulasi kalsium oksalat; Tempuyung; Akumulasi kalsium oksalat + Tempuyung; dan Akumulasi kalsium oksalat Tempuyung Simultan. Induksi akumulasi kalsium oksalat dilakukan menggunakan umbi porang ( Amorphophallus muelleri ) dengan dosis 6 mg/100 g BB selama tiga bulan secara oral. Pemberian daun tempuyung ( S. arvensis ) diberikan dengan dosis 3,3 mg/g BB selama tujuh hari. Serum darah diisolasi untuk dilakukan analisis kadar kreatinin. Plasma darah diisolasi untuk uji MDA plasma dengan metode TBARS. Organ ginjal diisolasi untuk pembuatan preparat organ untuk analisis akumulasi CaOx dengan pewarnaan Pizzolato, pengamatan ekspresi MDA dengan metode immunohstokimia menggunakan anti-MDA antibodi, serta pengamatan nekrosis sel dengan pewarnaan Hematoksilin Eosin. Data yang diperoleh dianalisa dengan menggunakan uji ANOVA selang kepercayaan 95% dan dilakukan analisis Path menggunakan SPSS 16 for windows . Berdasarkan hasil yang didapatkan diketahui bahwa induksi akumulasi kalsium oksalat yang diberikan serta pemberian daun tempuyung ( S. arvensis ) tidak berpengaruh terhadap kadar kreatinin serum darah (P 0.05). Hal ini diduga bahwa induksi akumulasi kalsium oksalat yang diberikan belum sampai menyebabkan nefrolithiasis. Pemberian daun tempuyung ( S. arvensis ) secara signifikan mampu menurunkan ekspresi MDA pada jaringan ginjal (P 0.05). Namun, pemberian daun tempuyung ( S. arvensis ) belum mampu dalam menurunkan kadar MDA plasma. Pemberian daun tempuyung ( S. arvensis ) juga mampu menurunkan jumlah sel yang mengalami nekrosis secara signifikan (P 0.05). Kandungan antioksidan dalam daun tempuyung ( S. arvensis ) diduga mampu menetralkan radikal bebas yang terbentuk akibat interaksi sel dengan oksalat, dan juga mampu melindungi sel-sel ginjal dari kerusakan akibat radikal bebas. Selain menurunkan radikal bebas dan nekrosis sel, pemberian daun tempuyung ( S. arvensis ) juga mampu meluruhkan akumulasi kalsium oksalat yang terbentuk secara signifikan (P 0.05). Sehingga, daun tempuyung ( S. arvensis ) mampu menurunkan akumulasi kalsium oksalat dengan cara menurunkan radikal bebas, melindungi sel dari kerusakan sel, serta meluruhkan akumulasi kalsium oksalat yang terbentuk. Dari hasil analisis uji Path, diketahui bahwa nekrosis sel ginjal dipengaruhi oleh MDA plasma (34.6%) dan MDA ginjal (46.9%), dimana 15.6% lainnya dipengaruhi oleh faktor lain. Sedangkan akumulasi kalsium oksalat dipengaruhi oleh MDA ginjal (47.4%) dan nekrosis sel ginjal (44.9%), dimana 7.5% lainnya dipengaruhi oleh faktor lain.
English Abstract
Accumulation of calcium oxalate is one of main factors that causing kidney stones (nephrolithiasis). presence of oxalate in kidneys can lead an increase of free radicals through lipid peroxidation reactions and cause necrosis cell. Tempuyung leaves ( S. arvensis ) has long been used as traditional medicine for kidney stone shed. However, shed of calcium oxalate accumulation until now is still unknown. aims of this study was to determine mechanism of tempuyung leaves ( S. arvensis ) in reducing accumulation of calcium oxalate, renal MDA expression, plasma free radicals, blood creatinine, and necrosis of kidney cells of mice ( M. musculus ) were induced accumulation of calcium oxalate. This research using mice ( M. musculus ) Swiss strain with an average body weight of 25-30 g as an animal model. experimental animals were divided into five groups: Normal; accumulation of calcium oxalate; Tempuyung; Accumulation of calcium oxlaate + Tempuyung, and accumulation of calcium oxalate Simultaneous with Tempuyung. Induction of calcium oxalate accumulation were conducted by using porang bulbs ( Amorphophallus muelleri ) at 6 mg/100 g BW dose by orally for three months. tempuyung leaves ( S. arvensis ) were given at 3.3 mg/g BW dose for seven days. Serum was isolated for analysis of blood creatinine levels. Blood plasma was isolated to test MDA plasma by TBARS method. kidney were isolated for making histological section for analysis accumulation of calcium oxalate by Pizzolato`s staining, observation of MDA expression by using immunohstokimia method with anti-MDA antibodies, observation of cell necrosis with hematoxylin eosin staining. data obtained were analyzed using ANOVA with confidence interval 95% and Path analysis using SPSS 16 for windows. Based on results is known that induced accumulation of calcium oxalate and treatment with tempuyung leaves ( S. arvensis ) had no effect on serum creatinine levels (P 0.05). It is suspected that accumulation of calcium oxalate induction has not given up causing nefrolithiasis. given of tempuyung leaves ( S. arvensis ) can significantly reduce expression of MDA in renal tissue (P 0.05). However, given tempuyung leaves ( S. arvensis ) has not been able to reduce levels of plasma MDA. provision tempuyung leaves ( S. arvensis ) is also able to reduce number of cells undergoing necrosis were significantly (P 0.05). Antioxidants in tempuyung leaves ( S. arvensis ) allegedly able to neutralize free radicals that formed by interaction of cells with oxalate, and also can protect kidney cells from free radical damage. In addition to lowering free radicals and cell necrosis, giving of tempuyung leaves ( S. arvensis ) is also able to shed accumulation of calcium oxalate was significantly (P 0.05). Thus, tempuyung leaf ( S. arvensis ) is able to reduce accumulation of calcium oxalate by free radicals, protecting cells from damage cells, and shed accumulation of calcium oxalate that formed. Based on Path analysis, it is known that renal cell necrosis is influenced by MDA Plasma (34.6%) and kidney MDA (46.9%), of which 15.6% are influenced by o r factors. While accumulation of calcium oxalate influenced by kidney MDA (47.4%) and renal cell necrosis (44.9%), where 7.5% is influenced by o r factors.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/615.399/ROS/s/041307529 |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs |
| Divisions: | S2/S3 > Magister Biologi, Fakultas MIPA |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 03 Feb 2014 16:04 |
| Last Modified: | 03 Feb 2014 16:04 |
| URI: | http://repository.ub.ac.id/id/eprint/158171 |
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