Dewi, Ardhia Deasy Rosita (2016) Studies on The Antioxidant Activity of Garlic (Allium sativum) Water-soluble Constituents. Magister thesis, Universitas Brawijaya.
Abstract
Kelimpahan sel radikal bebas mengarah pada metabolisme seluler normal yang terganggu pada akhirnya berbagai penyakit. Bawang putih dianggap sebagai agen medis yang paling umum yang mengandung organosulfurandfytochytochemical compound diseass. Ini adalah sumber alami yang memiliki konstituen organosulfur berlimpah. Organosulfur memiliki efek kesehatan yang menguntungkan terutama, menghambat spesies oksigen reaktif (ROS). Menariknya, di antara konstituen yang ada di Bawang Putih, N-Acetylcysteine (NAC) memiliki aktivitas antioksidan tertinggi saat menggunakan analisis pemulungan radikal DPPH. Kehadiran NAC dalam larut air bawang putih dapat menyelesaikan senyawa bioaktif dalam larut air bawang putih menangkal ROS. Konstituen yang larut dalam air bawang putih diidentifikasi oleh LC-MS dan 5 konstituen ditemukan, yaitu n-asetylcysteine, cysteinyl-alanine, phenol-2-2-benzoxazolyl dan 2 konstituen tidak diketahui. GC-MS Chromatogram juga menunjukkan 3 konstituen utama yang hadir dalam minyak bawang putih asdielyldisulphide (ayah) 53,81%, diallyltrisulphide (dats) 31,49% dan d-limonene 14,71%. Selanjutnya, aktivitas antioksidan ditentukan dengan menggunakan uji DPPH menunjukkan bahwa ekstrak larut air bawang putih memiliki aktivitas pemulungan DPPH yang lebih tinggi 70 ± 0,02% dibandingkan dengan minyak bawang putih 58 ± 0,07%. Survival of Macrophage Cell Line RAW 264.7, menunjukkan bahwa gelar air bawang putih diberikan: M.Sc Penasihat: Wen-Ling Shih, PH.D IV larut dalam IC10 355μg / ml kurang beracun daripada minyak bawang putih IC10 5.37 μg / mL. Hydroperoksida diterapkan untuk menginduksi spesies oksigen reaktif (ROS) dalam sel mentah 264,7. Uji ROS membuktikan bahwa sel-sel produksi ROS secara signifikan berkurang dalam ekstraksi larut air bawang putih dengan minyak bawang putih FSU. Ekstraksi larut air bawang putih dan minyak bawang putih penting untuk mengurangi stres oksidatif dengan mengaktifkan enzim antioksidan terutama HO-1. Waktu maksimum untuk aktivasi HO-1 melalui induksi NRF2 dicapai pada 4 jam ketika 10 μg / ml minyak garlic dan 400 μg / ml larut air bawang putih diinduksi.
English Abstract
abundance of free radical in cells leads to disrupted normal cellular metabolism eventually various diseases. Garlic is regarded as most common medical agent containing organosulfurandphytochemical compoundsagainst diseases. It is a natural source which has abundant organosulfur constituents. Organosulfur has beneficial health effects particularly, inhibiting Reactive Oxygen Species (ROS). Interestingly, among constituents present in garlic, N-acetylcysteine (NAC) posseses highest antioxidant activity when using DPPH radical scavenging analysis. Presence of NAC in garlic water-soluble may complete bioactive compounds in garlic water-soluble counteracting ROS. Garlic water-soluble constituents were identified by LC-MS and 5 constituents were found, namely N-acetylcysteine, cysteinyl-alanine, phenol-2-2-benzoxazolyl and 2 constituents unknown. GC-MS chromatogram also showed 3 main constituents present in garlic oil asdiallyldisulphide (DADS) 53.81%,diallyltrisulphide (DATS) 31.49% and D-limonene 14.71%. Subsequently, antioxidant activity determined by using DPPH assay showed that garlic water-soluble extract had higher DPPH scavenging activity 70 ± 0.02% compared with garlic oil 58 ± 0.07%. survival of macrophage cell line RAW 264.7, indicated that garlic water- Degree Conferred: M.Sc Adviser: Wen-Ling Shih, Ph.D IV soluble IC10 355μg/ml was less toxic than garlic oil IC10 5.37μg/ml. Hydroperoxide was applied to induce Reactive Oxygen Species (ROS) in RAW 264.7 cells. ROS assay proved that ROS production cells were significantly reduced in garlic water-soluble extractcompared with garlic oil FSU. Garlic water-soluble extractand garlic oil are important for reduce oxidative stress by activating antioxidant enzymes mainly HO-1. Maximum time for activation of HO-1 through induction of Nrf2 was achieved at 4 h when 10 μg/ml ofgarlic oil and 400 μg/ml of garlic water-soluble were induced.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/615.324 33/DEW/s/2016/041606027 |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs |
| Divisions: | S2/S3 > Magister Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian |
| Depositing User: | Nur Cholis |
| Date Deposited: | 08 Dec 2016 14:16 |
| Last Modified: | 25 Jan 2022 07:12 |
| URI: | http://repository.ub.ac.id/id/eprint/158126 |
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5. LIST OF FIGURES.pdf Download (134kB) | Preview |
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4. TABLE OF CONTENT.pdf Download (69kB) | Preview |
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2. abstract english.pdf Download (64kB) | Preview |
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6. LIST OF TABLES.pdf Download (127kB) | Preview |
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3. ACKNOWLEDGEMENT.pdf Download (186kB) | Preview |
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10. CHAPTER IV.pdf Download (1MB) | Preview |
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7. CHAPTER I.pdf Download (223kB) | Preview |
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11. CHAPTER V.pdf Download (224kB) | Preview |
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9. CHAPTER III.pdf Download (367kB) | Preview |
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COVer 2.pdf Download (38kB) | Preview |
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12. CONCLUSION.pdf Download (63kB) | Preview |
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中文摘要 chinese.pdf Download (125kB) | Preview |
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13. REFERENCES.pdf Download (307kB) | Preview |
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14. appendix.pdf Download (174kB) | Preview |
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