Caturrakhmadi, DonnyNoerhadiono (2013) Pengaruh Pemberian Ekstrak Daun Sirsat (Anona muricata) terhadap Penurunan Osteoklastogenesis pada Kultur Osteoklas Model Osteoartritis. Magister thesis, Universitas Brawijaya.
Abstract
Obyektif: untuk mengetahui efek pemberian ekstrak daun Annona muricata (sirsat) terhadap proses osteoklastogenesis pada kultur osteoklas model osteoarthritis. Bahan dan metode: suatu studi penelitian, kultur differensiasi osteoklas dari monosit manusia penderita osteoarthritis dan manusia normal yang dipapar radikal bebas (H 2 O 2 ) dan ekstrak ethanol daun Annona muricata (sirsat). Jumlah osteoklas di hitung dari keduanya. Hasil: Terdapat perbedaan jumlah osteoklas dari kultur darah tepi penderita osteoarthritis dan manusia normal. Annona muricata berperan menurunkan jumlah osteoklas yang terbentuk ketika dipapar radikal bebas (H 2 O 2 ). Dosis of 5 ug/mL, 10 ug/mL, dan 20 ug/mL mampu menurunkan jumlah osteoklas pada penderita osteoarthritis dan manusia normal. Pada dosis 20 ug/mL ternyata mampu menurunkan jumlah osteoklas hingga menyerupai kondisi tanpa paparan radikal bebas. Sehingga disimpulkan bahwa dosis 20 ug/mL adalah dosis optimal untuk aktifasi NFATc-1 pada sel osteoklas dari kultur darah penderita osteoartritis. Kesimpulan: Ekstrak daun Annona muricata pada kultur osteoklas model osteoartritis yang dipapar radikal bebas (H 2 O 2 ) pada penelitian ini mampu menurunkan osteoklastogenesis. Annona muricata mengindikasikan memiliki efek proteksi dalam menghambat radikal bebas pada keadaan osteoarthritis.
English Abstract
Objective: to determine the effect of Annona muricata leaf extract on osteoclastogenesis in osteoclast culture model of osteoarthritis. Material and methods: an experimental study, a culture of osteoclast differentiation derived from human monocytes of osteoarthritis patient and normal individual were exposed to free radicals (H 2 O 2 ) and Annona muricata ethanol leaf extract. The number of osteoclast are counted from both. Results: Different number of osteoclast obtained from blood cultures of osteoarthritis patient and normal individual. Annona muricata role lower number of osteoclast that form when exposed to free radicals (H 2 O 2 ). Doses of 5 ug/mL, 10 ug/mL, and 20 ug/mL were all able to reduce the number of osteoclast on both blood cultures of osteoarthritis patient and normal individual. Annona muricata leaf extract was capable to reduce osteoclast number at level similar to condition without free radical exposure at 20ug/mL dosage. Thus concluded a dose of 20 ug/mL is the most optimal dose for NFATc-1 activation in osteoclast cells from blood cultures of osteoarthritis patient. Conclusion: Leaf extract of Annona muricata on osteoclast culture model of osteoarthritis which was exposed to free radicals (H 2 O 2 ) in this study may lead to a decrease osteoclastogenesis. Annona muricata indicated having a protective effect against free radicals in influencing the state of osteoarthritis.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/615.323 22/CAT/p/041307093 |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs |
| Divisions: | Profesi Kedokteran > Spesialis Orthopedi dan Traumatologi, Fakultas Kedokteran |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 09 Oct 2013 13:27 |
| Last Modified: | 09 Oct 2013 13:27 |
| URI: | http://repository.ub.ac.id/id/eprint/158038 |
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