Wahyuni, EllyDwi (2014) Kombinasi Vitamin C dan E Meningkatkan Ekspresi Reseptor Estrogen α dan Ketebalan Endometrium Tikus yang Dipajan Monosodium Glutamat. Magister thesis, Universitas Brawijaya.
Abstract
Monosodium glutamat merupakan garam sodium dari asam amino glutamat. Konsumsi monosodium glutamat berlebihan dapat meningkatkan glutamat dalam plasma darah dan secara kimia bersifat neurotoksik sehingga menyebabkan terjadinya radikal bebas dalam tubuh. Radikal bebas mampu diredam oleh antioksidan vitamin C dan vitamin E yang bertindak sebagai scavenger terhadap radikal bebas yang terbentuk sehingga dimungkinkan tidak terjadi gangguan pada organ dan fungsi sel. Bertujuan untuk mengetahui pengaruh kombinasi vitamin C dan vitamin E tehadap ekspresi RE-α dan ketebalan endometrium pada tikus rattus novergicus strain wistar yang dipajan MSG. Penelitian eskperimental dengan rancangan randomized post test control group design menggunakan 25 ekor tikus betina dibagi secara acak menjadi lima kelompok. Kelompok kontrol negatif, kontrol positif (MSG saja), kelompok I (MSG 140mg/200gBB, vitamin C 0,2mg/gBB dan vitamin E 0,04 IU/gBB), kelompok II (MSG 140mg/200gBB, vitamin C 0,4mg/gBB + vitamin E 0,04 IU/gBB) kelompok III (MSG 140mg/200gBB, vitamin C 0,8mg/gBB dan vitamin E 0,04 IU/gBB) perlakuan diberikan peroral selama 42 hari. Jumlah sel yang mengekspresi RE-α pada uterus diperiksa menggunakan metode flowcytometri dan ketebalan endometrium diperiksa dengan Haemotoksilin dan Eosin (HE). Data analisis dengan uji one way ANOVA diikuti uji post hoc test LSD menunjukkan bahwa kombinasi vitamin C dan E memberikan pengaruh yang signifikan mencegah penurunan ekspresi RE-α (p=0,010) dan ketebalan endometrium (p=0,023). Uji korelasi pengaruh kombinasi vitamin C dan E pada berbagai dosis terhadap ekspresi REα r=0,592 p=0,006 dan ketebalan endometrium r=0,604 p=0,005 pada tikus yang dipapar MSG. Disimpulkan bahwa kombinasi vitamin C dan vitamin E dapat meningkatkan ekspresi RE-α dan ketebalan endometrium tikus yang dipajan MSG secara signifikan.
English Abstract
Monosodium glutamate is a sodium salt derived from glutamate amino acid in glutamate form. Overconsumption of this substance will cause an increase of glutamate in blood plasma, and due to its neurotoxin trait, it will chemically spread free radicals in the body. Free radicals can be inhibited by antioxidants, such as vitamin C and E, acting as scavenger against the formed free radicals and prevent any damage on the organs or cell functions. This research aimed to determine the effect of Vitamin C and E combination against RE-α expression and endometrial thickness on wistar strain rattus norvegicus mice exposed with MSG. The research was an experimental design using randomized post-test control group design with 25 female mice that were randomly distributed into five groups. Negative control group, positive control group (treated only with MSG), group I (with 140mg/200g W MSG, 0.2 mg/g W of vitamin C, and 0.04 IU/gW of vitamin E), group II (with 140mg/200gW MSG, 0.4 mg/gW of vitamin C, and 0.04 IU/grW of vitamin E), and group III (with140mg/200gW MSG, 0.8 mg/gW of vitamin C, and 0.04 IU/gW of vitamin E) were orally treated for 42 days. The quantity of cells expressing RE-α in the uterus were tested using flowcytometri method while endometrial thickness were examined by Haemotoxylin and Eosin (HE). Data were analyzed using one-way ANOVA followed by pos hoc test LSD, resulted in significant effect of Vitamin C and vitamin E combination in the decrease of RE-α expression (p=0.010) and endometrial thickness (p=0.023). Correlation test the effect of the combination of vitamins C and E at various doses on the expression RE-α r = 0.592 p = 0.006 and r = 0.604 endometrial thickness p = 0.005 at MSG exposed.It was concluded that the combination of vitamin C and vitamin E can increase the RE-α expression and endometrial thickness rat exposed by MSG. Thus, it is concluded that the combination of vitamin C and vitamin E may significantly lowering RE-α expression and endometrial thickness of mice that were exposed by MSG.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/612.399/WAH/k/041401545 |
| Subjects: | 600 Technology (Applied sciences) > 612 Human Physiology > 612.3 Digestive system |
| Divisions: | S2/S3 > Magister Kebidanan, Fakultas Kedokteran |
| Depositing User: | Hasbi |
| Date Deposited: | 03 Apr 2014 10:55 |
| Last Modified: | 03 Apr 2014 10:55 |
| URI: | http://repository.ub.ac.id/id/eprint/157976 |
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