Sutarjo, GanjarAdhyWirawan (2014) Pengaruh Penambahan Dimethyl Sulfoxide (DMSO) terhadap Kualitas Telur Ikan Mas (Cyprinus carpio Linn.) dalam Proses Kriopreservasi. Magister thesis, Universitas Brawijaya.
Abstract
Kriopreservasi merupakan salah satu teknik untuk mendukung ketersediaan benih ikan Mas ( Cyprinus carpio Linn.), karena teknik kriopreservasi mampu menyimpan sperma dan telur ikan dalam kurun waktu yang lama dengan penggunaan suhu yang sangat rendah. Tujuan penelitian adalah untuk mengetahui dan menganalisis pengaruh krioprotektan Dimethyl sulfoxide (DMSO) terhadap kualitas telur ikan Mas ( Cyprinus carpio Linn.), dan untuk mendapatkan dosis krioprotektan Dimethyl sulfoxide (DMSO) terbaik terhadap kualitas telur ikan Mas ( Cyprinus carpio Linn.) dalam proses kriopreservasi. Metode penelitian menggunakan metode eksperimen dengan rancangan percobaan acak lengkap. Perlakuan pertama adalah Krioprotektan DMSO 0,5 M, dan ekstender Madu 10 %, Perlakuan kedua Krioprotektan DMSO 1 M, dan ekstender Madu 10%, Perlakuan ketiga Krioprotektan DMSO 1,5 M ,dan ekstender Madu 10%, Perlakuan keempat Krioprotektan DMSO 2 M, dan ekstender Madu 10%, dan Perlakuan Kontrol tanpa krioprotektan DMSO, dan ekstender Madu 10%. Hasil penelitian menunjukan bahwa Fertilisasi telur ikan mas tertinggi pada kontrol atau tanpa perlakuan DMSO yaitu sebesar 84,98% dan diikuti oleh perlakuan B (DMSO 1 M) sebesar 76,26%, sedangkan fertilisasi terendah pada perlakuan D (DMSO 2 M) yaitu 10,97%. Daya tetas telur ikan mas tertinggi pada perlakuan control atau tanpa penambahan DMSO dan madu yaitu sebesar 90,93%, selanjutnya perlakuan B (DMSO 1 M) sebesar 75,89%, sedangkan derajat penetasan terendah pada perlakuan D (DMSO 2 M) yaitu 35,50%. Keberhasilan daya tetas telur yang tinggi pada perlakuan B (DMSO 1 M) dapat dipengaruhi oleh beberapa faktor yang meliputi kualitas telur, kualitas air dan penanganan pada saat penetasan. Sintasan tertinggi pada perlakuan B (DMSO 1 M) yaitu sebesar 59,70% dan sintasan terendah pada perlakuan D (DMSO 2 M) sebesar 20,60%. Krioprotektan Dimethyl sulfoxside (DMSO) diketahui berperan dalam menjaga keutuhan, dan mempertahankan kualitas sel telur ikan mas, hal tersebut di dasarkan pada keadaan morfologi telur seperti kondisi membran sel, zona pelusida, dan kemampuan sel telur terfertilisasi.
English Abstract
Cryopreservation is a technique to support the availability of carp fish seeds ( Cyprinus carpio Linn.), because cryopreservation techniques is capable to store fish sperm and eggs in a long period of time with the use of very low temperatures. The purpose of this study is to investigate and analyze the effect of Dimethyl sulfoxide cryoprotectants (DMSO) against the quality of carp fish eggs ( Cyprinus carpio Linn.), and to get the right dose of Dimethyl sulfoxide cryoprotectants (DMSO) for the quality of carp fish eggs ( Cyprinus carpio Linn. ) in the cryopreservation process. The method used is an experimental method with the experimental design was a complete randomized design. The first treatment is cryoprotectant DMSO 0,5 M and 10% Extender Honey, the second treatment is Cryoprotectant DMSO 1 M, and 10% Extender Honey, the third treatment is 1,5 M Cryoprotectant DMSO, and 10% Extender Honey, the fourth treatment is Cryoprotectant DMSO 2 M, and 10%, Extender Honey, and Cryoprotectants DMSO without treatment control, and 10% Extender Honey. The result showed that fertilization of carp eggs high in the untreated control or DMSO in the amount of 84.98%, followed by treatment B (DMSO 1 M) was 76.26%, while the lowest fertilization in treatment D (DMSO 2 M) is 10 , 97%. Carp egg hatchability highest in the control treatment or without the addition of DMSO and honey that is equal to 90.93%, further treatment B (DMSO 1 M) was 75.89%, while the lowest hatching in treatment D (DMSO 2 M) is 35 , 50%. The success of high hatchability of eggs in treatment B (DMSO 1 M) may be influenced by several factors including the quality of the eggs, water quality and treatment at the time of hatching. The highest survival rate in treatment B (1 M DMSO) that is equal to 59.70% and the lowest survival rate in treatment D (DMSO 2 M) of 20.60%. Cryoprotectants Dimethyl sulfoxide (DMSO) is known to play a role in maintaining the integrity, and maintain the quality of carp egg, it is based on the state of the egg morphology such conditions the cell membrane, the zona pellucida, and the ability of fertilized egg cells.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/597.483/SUT/p/041403672 |
| Subjects: | 500 Natural sciences and mathematics > 597 Cold-blooded vertebrates > 597.4 Miscellaneous superorders of Actinopterygii |
| Divisions: | S2/S3 > Magister Bioteknologi Perikanan dan Kelautan, Fakultas Perikanan dan Ilmu Kelautan |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 12 Aug 2014 15:48 |
| Last Modified: | 12 Aug 2014 15:48 |
| URI: | http://repository.ub.ac.id/id/eprint/157868 |
Actions (login required)
 |
View Item |