Rosyida, Novia Nur (2013) Studies of Biological Properties of Major Bioactive Compounds from the Stems of Crossostephium chinense (L.) Makino. Magister thesis, Universitas Brawijaya.
Abstract
Crossostephium Chinense L., umumnya dikenal sebagai Hai-fu-Rung, secara alami didistribusikan di dekat daerah pesisir pada formasi batu kapur dan karang di wilayah Taiwan dan Selatan di wilayah Asia dan digunakan sebagai sumber baru obat herbal. Tanaman ini adalah salah satu TCM terkenal (pengobatan tradisional Tiongkok) yang secara tradisional digunakan untuk mengobati pilek, campak, rematik, dan bronkitis. Ekstrak seluruh tanaman C. Chinense telah dilaporkan untuk menunjukkan beragam kegiatan biologis seperti anti-proliferasi, hepatoproteksi, dan anti-peradangan dengan efek amelioratif, tetapi hanya beberapa penelitian ini yang menyebutkan aktivitas antimikroba, antioksidan, dan antihipertensi terutama dari bagian batang. Menariknya, bahkan C. Chinense adalah obat herbal yang terkenal, tetapi masih jarang, terancam di alam liar, dan perlu diselidiki lebih lanjut terutama untuk bagian batangnya. Karenanya, penelitian ini dilakukan untuk menyelidiki senyawa bioaktif utama tanaman ini. Sepuluh senyawa utama diperoleh dari ekstrak metanol tanaman ini dan diidentifikasi sebagai: lupeol, β -sitostenone, Octadecyl (e) - P -Coumarate, cycleucalenol, campuran asam zat bulat dan asam ursolik, Betulin, Betulin, ergone dan 24-ethyl-5α -cholest-7-en- 3β -ol. Selanjutnya, beberapa tes biologis seperti aktivitas antimikroba, antioksidan, antihipertensi, sitotoksik, dan hepatoprotektif dievaluasi dengan uji zona penghambat, uji DPPH, uji penghambatan ACE, uji MTT, dan uji T -BHP.
English Abstract
Crossostephium chinense L., commonly known as Hai-Fu-Rung, was naturally distributed near coastal areas on limestone and coral formations in Taiwan and South east of Asia`s region and was used as a new source of herbal medicine. This plant is one of the famous TCM (Traditional Chinese Medicine) that was traditionally used for treating colds, measles, rheumatic, and bronchitis. Extracts of whole plant of C. chinense have been reported to exhibit diverse biological activities such as anti-proliferation, hepatoprotection, and anti-inflammation with ameliorative effect, but only a few of these studies mentioned its antimicrobial, antioxidant, and antihypertensive activity especially from stem part. Interestingly, even C. chinense is a famous herbal medicine, but it is still rare, threatened in the wild, and need further investigation especially for its stem part. Hence, this study was conducted to investigate the major bioactive compounds of this plant. Ten major compounds were obtained from the methanol extract of this plant and identified as: lupeol, β -sitostenone, octadecyl ( E )- p -coumarate, cycloeucalenol, a mixture of oleanolic acid and ursolic acid, stigmastan-3-one, betulin, ergone and 24-ethyl- 5α -cholest-7-en- 3β -ol. Furthermore, some biological assays such as antimicrobial, antioxidant, antihypertensive, cytotoxic, and hepatoprotective activities were evaluated by inhibitory zone assay, DPPH assay, ACE inhibitory assay, MTT assay, and t -BHP assay, respectively.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/583.99/ROS/s/041309013 |
| Subjects: | 500 Natural sciences and mathematics > 583 Magnoliopsida (Dicotyledons) > 583.9 Asteridae |
| Divisions: | S2/S3 > Magister Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 06 Feb 2014 16:03 |
| Last Modified: | 31 Mar 2022 07:46 |
| URI: | http://repository.ub.ac.id/id/eprint/157806 |
![[thumbnail of 041309013.pdf]](http://repository.ub.ac.id/style/images/fileicons/text.png) |
Text
041309013.pdf Download (4MB) |
Actions (login required)
 |
View Item |