Hasan, YulionoTrikaNur (2013) Efek Ekstrak Kacang Tunggak (Vigna unguicula) terhadap Ekspresi VEGF Kornea Model Inflamasi pada Tikus Strain Wistar (Rattus novergicus). Magister thesis, Universitas Brawijaya.
Abstract
Tujuan : Untuk mengetahui efek esktrak methanol kacang tunggak (Vigna unguiculata) terhadap ekspresi vascular endothelial growth factor (VEGF) kornea model inflamasi yang diinduksi oleh larutan kimia basa. Metode : Desain penelitian adalah interventional experimental study . Enampuluh tiga ekor tikus yang dipilih secara acak, 3 tikus sebagai kontrol negatif, 60 tikus diinduksi dengan NaOH 1 M (induksi inflamasi) dan diberikan 25 μM, 50 μM and 100 μM isoflavonoid genistein ekstrak kacang tunggak sebanyak empat kali sehari. Induksi basa menggunakan kertas saring yang dibasahi NaOH 1 M dan diletakkan pada puncak kornea mata kanan selama 60 detik. Aquabidest digunakan sebagai kontrol positif dan ekstrak kacang tunggak diberikan setelah induksi basa. Ekspresi VEGF diobservasi pada jam 6, 24, 48, 96 dan 168 menggunakan metode imunohistokimia. Hasil : Masing-masing dosis pemberian secara signifikan menurunkan ekspresi VEGF (p = 0,00) tetapi tidak berbeda berdasarkan waktu pengamatan (p = 0,033). Interaksi antara waktu pemberian dan dosis mempengaruhi ekspresi VEGF (R Square ( r 2 ) = 24,7 %). Regresi linear antara ekspresi VEGF dan dosis pemberian menghasilkan estimated effective dose (ED) dengan persamaan Y = 16.486 – 0.079 X (Y= ekspresi VEGF, X = dosis pemberian). Mempertimbangkan ekspresi VEGF pada kornea normal adalah 12 maka dosis efektif ekstrak methano kacang tunggak adalah 61.94 μM. Kesimpulan : Ekstrak methanol kacang tunggak menurunkan ekspresi VEGF pada kornea model inflamasi tetapi tidak berpengaruh berdasarkan lama pemberian. Dosis ekstrak methanol kacang tunggak 50 μM hampir sama dengan dosis efektif 61.94 μM.
English Abstract
Purpose : to evaluate the effects of methanolic extract of Vigna unguiculata administration on vascular endothelial growth factor (VEGF) expression in rats with corneal-alkali induced inflammation. Methods : This was an interventional experimental study. Sixty three rats were randomly choosen, 3 rats as control, 60 rats were treated with NaOH 1 M (as inflammation model) and administered with 25 μM, 50 μM and 100 μM of genistein in Vigna ungucuilata extract four times daily. Alkali burn by NaOH 1 M infiltration using filter paper applicated on the center cornea of right eye for 60 seconds. Aquabidest as positive control and Vigna ungucuilata extract treatment perfused immediately after alkali burn. VEGF observed at 6, 24, 48, 96 and 168 hours by immunohistochemical method. Results : Each dose of Vigna unguiculata extract administration significantly decreased the VEGF expression (p = 0,00). Differences of VEGF expression based on administration time were not statistically significant (p = 0,033). Interaction between time of administration and dose of administration had influenced the VEGF expression (R Square ( r 2 ) = 24,7 %). The Linear regression between VEGF expression and administration dose resulted in estimated effective dose (ED) with Y = 16.486 – 0.079 X (Y= VEGF expression, X = dose of administration). Considering VEGF expression in normal cornea was 12, we found the effective dose of methanolic Vigna unguiculata extract was 61.94 μM. Conclusion : Methanolic extract of Vigna unguiculata decreased the VEGF expression on alkali burn corneal inflammation in rats but no differences in VEGF expression based on time administration. Dose of 50 μM genistein in methanolic extract of vigna unguiculata was similar to effective dose 61.94 μM.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/583.74/HAS/e/041301353 |
| Subjects: | 500 Natural sciences and mathematics > 583 Magnoliopsida (Dicotyledons) > 583.7 Rosidae |
| Divisions: | Profesi Kedokteran > Spesialis Ilmu Kesehatan Mata, Fakultas Kedokteran |
| Depositing User: | Endro Setyobudi |
| Date Deposited: | 04 Apr 2013 09:07 |
| Last Modified: | 04 Apr 2013 09:07 |
| URI: | http://repository.ub.ac.id/id/eprint/157788 |
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