Kurniawan, Sendy (2016) Biosensor Konduktometri untuk Penentuan Kadar Urea Menggunakan Urease yang Teramobilisasi pada SPCE-Kitosan. Sarjana thesis, Universitas Brawijaya.
Abstract
Penelitian ini mempelajari pengaruh ketebalan membran dan pH larutan urea terhadap kinerja biosensor. Ketebalan membran mempengaruhi waktu migrasi ion hasil hidrolisis urea ke elektroda dan pH larutan mempengaruhi aktivitas urease. Kinerja biosensor diamati menggunakan ketebalan membran 20; 40; 73; dan 110 μm dengan pH larutan urea 7,0; 7,5; 8,0; dan 8,5. Hasil penelitian ini menunjukkan bahwa ketebalan membran dan pH larutan mempengaruhi kinerja biosensor. Kinerja biosensor optimum dihasilkan pada ketebalan membran 40 μm dan pH larutan urea 7,0. Pada kondisi tersebut biosensor memiliki kisaran konsentrasi 0 – 0,9 ppm dengan batas deteksi 0,05 ppm dan kepekaan 47 μS/ppm.
English Abstract
This research was studied about the effect of membrane thickness and pH of urea solution to the performance of biosensor . Membrane thickness affect time of ion migration which produce in hydrolysis urea to electrode and pH solution affect the result of urea hydrolysis. The performance of the biosensor was monitored using membrane thickness 20; 40; 73; and 110 μm with pH urea solution 7.0; 7.5; 8.0; and 8.5. Result of this research showed that membrane thickness and pH of urea solution affected performance of biosensor. Optimum performance of the biosensor was occurred in membrane thickness 40 μm and pH of urea solution 7.0. In that condition conductometric urea biosensor has concentration range 0 – 0.9 ppm urea with limit detection 0.05 ppm and sensitivity 47 μS/ppm.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2016/384/051610321 |
| Subjects: | 500 Natural sciences and mathematics > 540 Chemistry and allied sciences |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Kustati |
| Date Deposited: | 02 Nov 2016 14:37 |
| Last Modified: | 02 Nov 2016 14:37 |
| URI: | http://repository.ub.ac.id/id/eprint/154913 |
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