Mardiana (2014) Optimasi Amobilisasi Xilanase dari Trichoderma Viride Menggunakan matriks Bentonit. Sarjana thesis, Universitas Brawijaya.
Abstract
Penelitian ini bertujuan untuk mengetahui waktu pengocokan optimum, konsentrasi xilanase optimum, serta efisiensi pemakaian ulang xilanase amobil. Kadar dan aktivitas xilanase amobil ditentukan pada variasi waktu pengocokan (1, 2, 3, 4, 5) jam dan variasi konsentrasi xilanase (0,157; 0,209; 0,261; 0,314; 0,366) ppm pada o,1 g bentonit teraktivasi H2SO4. Penentuan kadar protein dilakukan dengan metode Spektrofotometri menggunakan reagen Biuret sedangkan aktivitas xilanase ditentukan dengan mengukur kadar xilosa yang dihasilkan selama reaksi enzimatis secra spektrofotometri menggunakan reagen DNS. Xilanase yang digunakan untuk amobilisasi mempunyai kadar protein 0,366 mg/mL dengan aktivitas 8,449 unit. Penentuan efisiensi xilanase amobil dilakukan dengan uji aktivitas sebanyak lima kali pengulangan. Hasil penenlitian menunjukkan bahwa kondisi optimum amobilisasi xilanase dicapai pada waktu pengocokan 3 jam dengan jumlah xilanase teradsorpsi 0,378 mg/0,1 g bentonit dan aktivitas 10,245 unit. Sedangkan pada variasi konsentrasi didapatkan konsentrasi xilanase 0,261 ppm dan aktivitas 14,528 unit (P<0,01). Xilanase amobil dapat digunakan sebanyak lima kali pengulangan dengan aktivitas sisa sebesar 51,20%.
English Abstract
This research aim to determine optimum agitation time, optimum xylanase concentration, and reuse efficency of ammobilized xylanase. Level and activity of immobilized xylanase were determined on variation of agitation time ( 1, 2, 3, 4, 5) hour, and variation of xylanase concentration (0,157; 0,209; 0,261; 0,314; 0,366) ppm on 0,1 g of H2SO4 activated bentonite. Determination of protein level was conducted by spectrophotometry method using biuret reagent, whereas xylanase activity was determined by measuring xylosa level which was obtained on enzymatic reaction by spectrophotometry using DNS reagent. Xylanase which was used for immobilization content 0,366 mg/mL protein level with 8,449 unit activity. Determination of immobilized xylanase efficiency was conducted by activity analyse for five time repitition. This research showed that the optimum condition of immobilized xylanase was obtained on 3 hour of agitation time with 0,378 mg adsorbed xylanase/0,1 g bentonite, and 10,245 unit activity. Whereas on vary of concentration, the optimum condition of immobilized xylanase was obtained on 0, 261 ppm and 14,528 unit (P<0,01). Immobilized xylanase can be used for five time repetition with 51,20% of residual activity.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2014/334/051405832 |
| Subjects: | 500 Natural sciences and mathematics > 540 Chemistry and allied sciences |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 15 Sep 2014 10:43 |
| Last Modified: | 21 Oct 2021 05:40 |
| URI: | http://repository.ub.ac.id/id/eprint/153968 |
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