Pratiwi, ErmaKusuma (2013) Uji Toksisitas Bacillus Thuringiensisasal Kota Nganjuk Terhadap Larva Aedes Aegypti. Sarjana thesis, Universitas Brawijaya.
Abstract
Demam Berdarah Dengue (DBD) merupakan salah satu penyakit endemik di Indonesia dan menyebabkan banyak kematian penduduk, khususnya di Provinsi Jawa Timur. Kota Nganjuk memiliki tingkat kelimpahan larva Aedes aegypti tertinggi di Provinsi Jawa Timur pada tahun 2008-2010. Kota Nganjuk merupakan daerah fokus utama untuk penanganan penyakit endemik DBD di Indonesia.Tujuan penelitian ini untuk mendapatkan isolat Bacillus thuringiensisasal Kota Nganjuk yang paling efektif dalam membunuh larva Aedes aegypti. Sampel sedimen dan air diambil dari 10 lokasi di Kecamatan Nganjuk. Bakteri diisolasi menggunakan media selektif B.thuringiensiskemudian dilakukan karakterisasi fenotip(Profil Matching Method), seleksi isolat yang patogen dan uji toksisitas (LC50) pada larva nyamuk Aedesaegypti instar III.Persentase mortalitas larva dianalisis probit(LC50) dan ragam (ANOVA). Hasil isolasi mendapatkan dua isolat B. thuringiensis yaitu K.K1.S.K2 dan W.Swh.S.K2 dari 26 isolat bakteri yang mampu membunuh larva Aedes aegypti instar III lebih dari 50 %. Isolat K.K1.S.K2 serta W.Swh.S.K2 dengan umur biakan 48 jam pada waktu pendedahan 72 jam efektif membunuh larva Aedes aegypti instar III secara berurutan yaitu 83,3 % dan 76,67 %. Isolat W.Swh.S.K2 hasil isolasi dari sampel sedimen memiliki toksisitas tertinggi dengan nilai LC50 48 jam sebesar 3,53x107 sel/ml.
English Abstract
Dengue Hemorrhagic Fever (DHF) is one of endemic disease in Indonesia and increase mortality of people, especially in East Java. Nganjuk has the highest density of Aedes aegypti larvae from all city in East Java at 2008-2010 caused of it. Nganjuk was the one of major DHF epidemic foci in Indonesia. The objective of this research was to get Bacillus thuringiensis isolates from Nganjuk City that has highest toxicity to Aedes aegypti larvae. The sediment and water samples were taken from 10 sites in Nganjuk. Bacterial isolation wasperformed using B. thuringiensis selective media.Phenotypic characteristics of the isolateswere obtained with the simple matching method, and the toxicity tests were also performed on the thirdinstar larval stage of Aedes aegypti.Larvae mortality was analyzed by using regression probit and value of LC50 was analyzed using ANOVA. Two indigenous B. thuringiensis isolates among the 26 bacterial isolates, K.K1.S.K2 and W.Swh.S.K2 were toxic to the third instar larvaeof A. Aegypti more than 50 % mortality. The K.K1.S.K2 and W.Swh.S.K2 isolates at the age of 48-hour culture at 72 hours of exposure time kill the third instar larvae of Aedes aegypti in a row 83,3% and 76,67%. W.Swh.S.K2 that isolated from sediment samplehas highest toxicityfor killing the third instar larvae of Aedes aegyptiat LC5048h = 3,53 x107 cells / mL.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2013/321/051308372 |
| Subjects: | 500 Natural sciences and mathematics > 570 Biology |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Hasbi |
| Date Deposited: | 30 Sep 2013 15:03 |
| Last Modified: | 25 Oct 2021 02:57 |
| URI: | http://repository.ub.ac.id/id/eprint/153584 |
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