Ridlo, Ahmad (2013) Dinamika Komunitas Dan Potensi Bakteri Pendegradasi Linear Alkilbenzen Sulfonat Pembentuk Biofilm Di Ekosistem Sungai Sunter. Sarjana thesis, Universitas Brawijaya.
Abstract
Pencemaran air di ekosistem sungai banyak disebabkan oleh penggunaan deterjen dengan surfaktan anionik Linear Alkylbenzen Sulfonat (LAS). Komunitas bakteri pembentuk biofilm indegenous sungai tercemar deterjen diketahui memiliki kemampuan mendegradasi LAS. Tujuan penelitian ini untuk mempelajari dinamika dan diversitas komunitas bakteri pembentuk biofilm di ekosistem sungai serta potensinya dalam mendegradasi LAS. Penelitian meliputi isolasi bakteri dari sedimen sungai dengan metode pour plate dan uji potensi biodegradasi dalam sistem biakan kontinyu. Konsorsium isolat-isolat bakteri hasil isolasi diuji potensinya dalam mendegradasi LAS dalam media mineral sederhana yang mengandung 20 mg/L LAS dengan penambahan sedimen batu sebagai substrat dan perlakuan debit 50 dan 70 mL/jam. Densitas komunitas bakteri pendegradasi LAS dari bulan Desember 2012 hingga Februari 2013 relatif sama yaitu antara 19,7x104 cfu/g – 28,9x104 cfu/g, dengan indeks diversitas kurang dari 0,4. Konsorsium bakteri pada perlakuan debit 50 dan 70 mL/jam secara berturutan dapat mendegradasi LAS sebesar 51,23 % dan 76 % dengan waktu inkubasi 24 jam. Didapatkan enam isolat bakteri pendegradasi LAS berdasarkan karakter fenotip berbeda spesies dengan nilai similaritas 51 %.
English Abstract
Water pollution in the river ecosystem was affected by the utilization of anionic detergents with Linear Alkylbenzene Sulfonate (LAS). Indegenous communities of biofilm bacteria in the detergent-polluted rivers was able to degrade LAS. The purpose of this research were to study the dynamics and diversity of biofilms bacteria communities in river ecosystems and to observe the potency of LAS degradation. The studies steps were isolation of bacteria from river sediments by pour plate method and assay of biodegradation in a continuous culture system. Consortium of bacterial isolates were assayed potency of LAS degradation using mineral salt medium containing 20 mg/L LAS with addition of stone as a living place of biofilm bacteria and debit medium 50 and 70 mL/hour. Density of biofilm bacteria community from December 2012 to February 2013 was relatively stable at 19.7 x104 cfu/g to 28.9 x104 cfu/g, with a diversity index of less than 0,4. Consortium of bacteria with medium debit 50 and 70 mL/hour able to degrade LAS as much as 51.23 % and 76 %, of respectively. Six isolates of biofilm bacteria based on phenotypic characters show that all isolate were different species with 51 % similarity.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2013/308/051308359 |
| Subjects: | 500 Natural sciences and mathematics > 570 Biology |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Hasbi |
| Date Deposited: | 30 Sep 2013 09:37 |
| Last Modified: | 25 Oct 2021 02:49 |
| URI: | http://repository.ub.ac.id/id/eprint/153569 |
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