Yulistian, DhoniPrielananta (2013) Studi Pengaruh Jenis Pelarut terhadap Hasil Isolasi dan Kadar Senyawa Fenolik dalam Biji Kacang Tunggak (Vigna unguiculata (L.) Walp) sebagai Antioksidan. Sarjana thesis, Universitas Brawijaya.
Abstract
Penelitian ini bertujuan untuk mengetahui pengaruh jenis pelarut terhadap kadar fenolik total dari ekstrak biji kacang tunggak beserta pengujian aktivitasnya sebagai bahan antioksidan. Uji fitokimia yang digunakan meliputi kadar fenolik total termasuk didalamnya kadar flavonoid total. Pengujian aktivitas antioksidan dilakukan dengan metode 2,2-difenil-1-pikrilhidrazil (DPPH). Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol, metanol, 70% etanol, 70% metanol, 80% aseton, 50% aseton, dan 0,5% asam asetat dalam 70% aseton (v/v). Hasil penelitian menunjukkan bahwa 80% aseton merupakan pelarut yang dapat mengekstrak senyawa fenolik paling besar dan memiliki aktivitas antioksidan terbesar juga. Kadar fenolik total dan flavonoid total yang dihasilkan mencapai 8.081,4 EAG/g sampel dan 33.308,3 EK/g sampel. Sedangkan aktivitas antioksidan yang terukur mencapai 620,9%. Hasil penelitian menunjukkan bahwa kepolaran pelarut mempengaruhi kadar senyawa fenolik yang terekstrak dan aktivitas antioksidan.
English Abstract
The aim of this research were to investigate the effect of different solvents toward total phenolic compounds and antioxidant activities of cowpea seed extract. Here, the total phenolic compound also including total flavonoid were investigated. Antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazil (DPPH) free radical scavenging. The solvents employed to extract the seed were ethanol, methanol, 70% ethanol, 70% methanol, 80% acetone, 50% aceton, and 70% acetone containing 0.5% acetic acid using maceration extraction. The result showed that 80% acetone was suitable solvent and gave the highest amounts of the total phenolic compounds and also the antioxidant activity. The total phenolic and total flavonoid contents from cowpea extract was 8,081.43 mg GAE/g and 33,308.3 mg QE/g sample, respectively. Moreover, the total antioxidant activity about 620.9%. These results suggested that the solvent polarity had significant effects on the total phenolic contents and antioxidant activity. Thus, the correlation between total phenolic contents and antioxidant activities were also well observed.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2013/233/051307213 |
| Subjects: | 500 Natural sciences and mathematics > 540 Chemistry and allied sciences |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Hasbi |
| Date Deposited: | 10 Sep 2013 09:16 |
| Last Modified: | 25 Oct 2021 02:26 |
| URI: | http://repository.ub.ac.id/id/eprint/153495 |
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