Thaati, Hayyunisa (2013) Optimasi Amobilisasi Xilanase dari Trichoderma viride pada Matriks Pasir Laut Terlapis Kitosan. Sarjana thesis, Universitas Brawijaya.
Abstract
Xilanase bebas hanya bisa digunakan satu kali reaksi, sehingga perlu diamobilisasi agar bisa digunakan berulang. Xilanase yang telah diisolasi dari Trichoderma viride diendapkan dengan metode fraksinasi bertingkat menggunakan amonium sulfat dengan tingkat kejenuhan 40-80% dan dilanjutkan dengan dialisis. Xilanase diamobilisasi dengan metode adsorpsi fisik menggunakan matriks pasir laut terlapis kitosan. Penelitian ini bertujuan mengetahui waktu pengocokan, konsentrasi xilanase optimum, serta efisiensi pemakaian ulang xilanase amobil. Pada penelitian ini dilakukan variasi waktu pengocokan (1, 2, 3, 4, 5) jam dan konsentrasi enzim sebesar (0,5; 1,5; 2,5; 3,5; 4,5) mg/mL pada 0,1 g pasir pada temperatur ruang dengan konsentrasi larutan kitosan 1,5% dan larutan Na5P3O10 3%. Kadar protein enzim diuji secara spektrofotometri dengan reagen Biuret dan gula pereduksi dengan reagen DNS. Kadar protein xilanase bebas diperoleh sebesar 4,5 mg/mL dengan aktivitas sebesar 17,0 unit. Hasil penelitian menunjukkan bahwa kondisi optimum amobilisasi xilanase pada pasir laut terlapis kitosan dicapai pada waktu pengocokan 3 jam dan konsentrasi xilanase 3,5 mg/mL dengan jumlah xilanase teradsorpsi 16,7 mg dan aktivitas 34,5 unit. Xilanase amobil ini secara efisien dapat digunakan hingga 5 kali pemakaian dengan aktivitas sebesar 51,74%.
English Abstract
Free xylanase can be used only in one reaction, so that needs to be immobilized in order to be reused. Xylanase isolated from Trichoderma viride was precipitated by using ammonium sulphate at 40-80% saturation level, followed by dialysis. Xylanase was immobilized by physical adsorption on a matrix of chitosan-coated sea sand. This study aimed to determine the shaking time, optimum concentration of xylanase, and the efficiency of immobilized xylanase. In this research, the shaking time was (1, 2, 3, 4, 5) hours and variation of xylanase concentration was (0.5; 1.5; 2.5; 3.5; 4.5) mg/mL at 0.1 g of sand, at room temperature, within the matrix of 1.5% chitosan solution and 3% Na5P3O10 solution. Protein content was tested spectrophotometrically by using Biuret reagent, while reduced sugar by DNS reagent. Initial protein content was 4.5 mg/mL and free enzyme activity was 17.0 units. The results showed that the optimum condition of xylanase immobilization on chitosan-coated sea sand was achieved on shaking time of 3 hours and xylanase concentration of 3.5 mg/mL yielding in 16.7 mg adsorbed xylanase within activity of 34.3 units. This immobilized xylanase can be efficiently used up to 5 times and the activity of 51.74%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2013/225/051307205 |
| Subjects: | 500 Natural sciences and mathematics > 540 Chemistry and allied sciences |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Hasbi |
| Date Deposited: | 10 Sep 2013 09:52 |
| Last Modified: | 25 Oct 2021 02:23 |
| URI: | http://repository.ub.ac.id/id/eprint/153486 |
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