Sari, AyundaArum (2013) Amobilisasi Pektinase dari Bacillus firmus Menggunakan Matriks Oxidized Polypropylene (OPP)-Kitosan. Sarjana thesis, Universitas Brawijaya.
Abstract
Pektinase adalah enzim yang dapat memecah senyawa pektin menghasilkan asam galakturonat. Untuk meningkatkan efisiensi pemakaian pektinase sehingga dapat dipakai berulang kali dapat dilakukan dengan teknik amobilisasi. Pektinase hasil isolasi dari Bacillus firmus dimurnikan menggunakan amonium sulfat dengan tingkat kajenuhan 20-60% dan dilanjutkan dengan dialisis. Penelitian ini bertujuan untuk menentukan kondisi optimum pektinase yang diamobilisasi secara adsorpsi fisik pada matriks oxidized polypropylene (OPP) terlapis kitosan yang meliputi lama pengocokan, konsentrasi enzim optimum dan efisiensi pemakaian ulang pektinase amobil. Lama pengocokan dan konsentrasi pektinase divariasi untuk menentukan kondisi optimum enzim pektinase. Penentuan kadar protein sisa dilakukan dengan menggunakan reagen Biuret dan kadar asam galakturonat hasil hidrolisis pektin menggunakan reagen DNS. Kadar protein pektinase bebas yang digunakan untuk amobilisasi 1,367 mg/ml dengan aktivitas 241,1 unit. Hasil penelitian menunjukkan bahwa lama pengocokan optimum dicapai pada lama pengocokan 3 jam dan konsentrasi pektinase 1,094 mg/mL dengan aktivitas sebesar 220,2 unit. Efisiensi pemakaian ulang pektinase amobil dengan matriks OPP terlapis kitosan dapat digunakan sebanyak 4 kali pengulangan dengan aktivitas sebesar 62,05%.
English Abstract
Pectinase is an enzyme that can hydrolyze pectin compounds into galacturonic acid. In order to enhance the pectinase efficiency, the enzyme can be immobilized in certain matrix. The pectinase was isolated from Bacillus firmus, and then purified with ammonium sulphate with 20-60% saturated level, followed by dialysis. The aims of the research were to determine the optimum conditions of immobilized pectinase by physical adsorption on oxidized polypropylene (OPP) coated chitosan, which included the shaking time, enzyme concentration and the efficiency of immobilized pectinase. Shaking time and concentration of pectinase varied to determine the optimum conditions of the pectinase immobilization. Determination of protein content was carried out by using Biuret reagent and galacturonic acid amount resulting from the hydrolysis of pectin by using DNS reagent. Initial protein used for immobilizing free pectinase was 1.367 mg/mL and the activity was 241.1 units. The results showed that the optimum condition of pectinase immobilization was achieved on shaking time of 3 hours and pectinase concentration of 1.094 mg/mL within activity of 220.2 units. Efficiency of immobilized pectinase using OPP coated chitosan matrix can be performed four repeations with activity of 62.05%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2013/202/051307182 |
| Subjects: | 500 Natural sciences and mathematics > 540 Chemistry and allied sciences |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Hasbi |
| Date Deposited: | 09 Sep 2013 10:04 |
| Last Modified: | 25 Oct 2021 02:16 |
| URI: | http://repository.ub.ac.id/id/eprint/153463 |
Preview |
Text
AYUNDA_SKRIPSI.pdf Download (3MB) | Preview |
Actions (login required)
 |
View Item |