AnangPriambowo (2010) Studi Histopatologi Pada Mukosa Lambung Mencit (Mus musculus) Setelah Paparan Formalin Subkronik. Sarjana thesis, Universitas Brawijaya.
Abstract
Tujuan penelitian ini adalah untuk mengetahui perubahan struktur lapisan mukosa lambung mencit (Mus musculus) galur BALB-c akibat paparan formalin. Mencit berumur 2 bulan dipapar formalin dengan konsentrasi 0 mg/kg BB, 2 mg/kg BB dan 5 mg/kg BB selama 3 bulan. Lambung bagian fundus diambil dan dilakukan pembuatan preparat melintang dan pewarnaan jaringan menggunakan hematoxilen-eosin. Hasil pengamatan pada mukosa bagian glandular menunjukkan terjadi perubahan struktur yaitu lapisan mukosa mengalami pelebaran jarak antar sel parietal, destruksi sel parietal dan chief sel dengan mengamati dan membandingkan pada mencit kontrol sebelum perlakuan, paparanan formalin 0 mg/kg BB, 2 mg/kg BB dan 5 mg/kg BB. Sel parietal dan chief sel yang mengalami destruksi dilakukan penghitungan titik lokasinya dan dilakukan analisis menggunakan analisis ragam ANOVA dan uji lanjutan Lower Significant Different (LSD) α=0.05. Hasil pengamatan dan penghitungan destruksi sel parietal dan chief sel secara statisistik terjadi peningkatan signifikan pada paparan 5 mg/kg. Sel parietal pada paparan formalin 5 mg/kg mengalami destruksi dengan nilai 12,7 unit atau meningkat sebesar 223,98% dari sebelum perlakuan. Sedangkan chief sel juga mengalami peningkatan yang signifikan pada paparan formalin 5 mg/kg dengan nilai sebesar 6,39 unit atau meningkat 102,85 % dari sebelum perlakuan.
English Abstract
The aim of this research is to know the changes of mucosa structure layers in gastric of mice (Mus musculus) strain BALB-c after formalin treatment. Two-months old mice was exposed by formalin per oral 0 mg/kg BW, 2 mg/kg BW and 5 mg/kg BW for three months. The fundic of gastric is taken away and processed by standard procedures and embedded in paraffin. Tissue were sectioned across and stained using hematoxilen and eosin. The result from this research show that the changes structure that is happened in gastric’s mukosa layers of widening of parietal cells and destruction of parietal cells with compare in mice’s condition before exposion, mice’s after exposion (concentration 0 mg/kg), 2 mg/kg and 5 mg/kg BW. Histophatological changes were observed in the glandular mucosa of stomach that the parietal cells and chief cells has destruction. Destruction parietal cells and chief cells was count. The result of calculation analyzed with one-way ANOVA and analized continue with Lower Significant Different (LSD) α=0.05. The observation and calculation with statistic analyze of destruction of parietal cells and chief cells, significantly happened in concentration 5 mg/kg. The destruction of parietal cells increase about 12,7 unit or increase 223,98% from normal condition before exposion. The destruction of chief cells has significantly happened and increase about 6,39 unit or increase 102,85% from normal condition before the exposition.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2010/40/051000477 |
| Subjects: | 500 Natural sciences and mathematics > 570 Biology |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Unnamed user with email repository.ub@ub.ac.id |
| Date Deposited: | 15 Mar 2010 10:07 |
| Last Modified: | 22 Oct 2021 06:52 |
| URI: | http://repository.ub.ac.id/id/eprint/152503 |
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