Semetisari, Ratna (2008) Studi Efek Puerarin terhadap Konsentrasi Tumor Necrosis Factor- (TNF-) pada Kultur Human Umbillical Vein Endothelial Cells (HUVECs) yang diinduksi Leptin. Sarjana thesis, Universitas Brawijaya.
Abstract
Studi terdahulu menguji bahwa leptin menyebabkan terjadinya disfungsi endotel yang merupakan patobiologi aterogenesis. Puerarin merupakan antioksidan yang diduga mampu menghambat mekanisme aterogenesis yang salah satunya dipicu oleh TNF- sebagai zat proinflamasi. Penelitian ini bertujuan untuk menguji dan menentukan berapa konsentrasi puerarin yang dapat menurunkan konsentrasi Tumor Necrosis Factor- (TNF-) pada kultur Human Umbillical Vein Endothelial Cells (HUVECs) yang diinduksi 25 ng/mL leptin. Kultur sel endotel dikelompokkan menjadi empat yaitu kultur sel endotel (1) 0 ng/mL dan 0 M puerarin, (2) diinduksi leptin konsentrasi 25 ng/mL selama 12 jam, (3) diinduksi puerarin masingmasing 5; 25; 200; dan 525 M selama 12 jam tanpa leptin, (4) diinduksi 25 ng/mL leptin bersama puerarin masing-masing 5; 25; 200; dan 525 M selama 12 jam. Selanjutnya dilakukan pengukuran konsentrasi TNF-a menggunakan prosedur dalam human TNF- ELISA Kit pada masing-masing medium kultur sel endotel. TNF-dibaca ELISA reader berdasarkan nilai Optical Density (OD). Konsentrasi TNF- dihitung dengan cara memplotkan nilai OD pada kurva standart TNF-. Data yang diperoleh dianalisis menggunakan SPSS dengan uji ANOVA satu arah dan uji lanjutan Tukey . Hasil penelitian menunjukkan bahwa 25 mM puerarin menurunkan konsentrasi TNF- pada kultur sel endotel vena umbilikus manusia (HUVECS) yang diinduksi leptin 25 ng/mL, sedangkan 200 mM dan 525 mM puerarin menurunkan konsentrasi TNF- mencapai keadaan normal pada kultur HUVECs yang diinduksi leptin 25 /mL.
English Abstract
The last study has proven that leptin caused the endothelial dysfunction as a step of the atherogenesis. Puerarin, an antioxidant, is presumed having the ability to block the atherogenesis mechanism, which is stimulated by TNF- as a proinflamatory cytokine. Therefore, the aim of this research was to prove and to determine whether puerarin could decrease the Tumor Necrosis Factor- (TNF-) concentration in Human Umbillical Vein Endothelial Cells (HUVECs) culture induced by 25 ng/mL leptin. In vitro study used primary culture of the HUVECs were devided in to four groups, there were (1) 0 ng/mL and 0 M puerarin, (2) induced by 25 ng/mL leptin for 12 hours, (3) induced by puerarin each 5; 25; 200 and 525 μM for 12 hours without leptin, (4) induced by leptin and puerarin with concentration 5; 25; 200 and 525 μM for 12 hours. Then the measurement of Tumor Necrosis Factor- (TNF-) concentrations was applied by using human TNF- ELISA Kit procedure on cell endothel culture medium. TNF- was read by ELISA reader based on Optical Density (OD) value. TNF- concentrations were estimated by plotting OD value in standard curve of TNF-. The results were analyzed using SPSS with one-way ANOVA test then followed by Tukey test 0.05. It was showed that puerarin 25 mM decreased TNF- concentration in Human Umbillical Vein Endothelial Cells (HUVECs) culture induced by leptin 25 ng/mL, however puerarin 200 mM and 525 mM decreased TNF- concentration in HUVECs culture induced by leptin 25 ng/mL until reach normallity.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2008/108/050801453 |
| Subjects: | 500 Natural sciences and mathematics > 510 Mathematics |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Matematika |
| Depositing User: | Unnamed user with email repository.ub@ub.ac.id |
| Date Deposited: | 15 Jul 2008 14:20 |
| Last Modified: | 09 Mar 2022 03:07 |
| URI: | http://repository.ub.ac.id/id/eprint/151763 |
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