Putra, IGusti Ngurahn Pratama (2015) Efek Hepatoprotektor Mie Berbasis Umbi-Umbian Lokal Terhadap Kondisi Stres Oksidatif Tikus Wistar (Rattus Norvegicus). Sarjana thesis, Universitas Brawijaya.
Abstract
Gangguan Fungsi Hati Kronik Disebabkan Salah Satunya Oleh Kerusakan Sel Yang Disebabkan Oleh Stres Oksidatif. Stres Oksidatif Muncul Akibat Paparan Radikal Bebas Dalam Tubuh Yang Dapat Bersumber Dari Induksi Minyak Jelantah. Hal Seperti Ini Akan Menyebabkan Peroksidasi Lemak, Rusaknya Molekul Lemak (Fosfolipid) Membran Sel, Rusaknya DNA Dan Oksidasi Protein. Umbi-Umbian Lokal Seperti Umbi Kimpul, Umbi Garut, Ubi Kelapa, Umbi Gadung Dan Umbi Gembili Yang Mengandung Dioskorin, Diosgenin Dan Fenol Diyakini Berfungsi Sebagai Antioksidan. Antioksidan Dapat Melawan Pengaruh Bahaya Dari Radikal Bebas. Disisi Lain, Umbi-Umbian Lokal Ini Jarang Diolah Dan Dikonsumsi Oleh Kalangan Masyarakat Karena Rasanya Yang Kurang Disukai. Oleh Karena Itu Umbi-Umbian Lokal Ini Diolah Menjadi Mie Sehat. Mie Ini Diharapkan Menjadi Alternatif Makanan Fungsional Bagi Masyarakat . Tujuan Dari Penelitian Ini Adalah Mengetahui Efek Hepatoprotektor Dari Mie Sehat Berbasis Umbi-Umbian Terhadap Kadar MDA, SOD, Aktivitas Katalase Dari Organ Hepar Tikus, Serta Gamabaran Histopatologi Pada Kondisi Stres Oksidatif Akibat Minyak Jelantah. Rancangan Penelitian Yang Digunakan Adalah Rancangan Acak Lengkap (RAL) Dengan 1 Faktor Terdiri Dari 8 Level Dan Diulang Sebanyak 5 Kali. Kedelapan Level Tersebut Yakni Kontrol Negatif (Normal), Kontrol Positif (Induksi Jelantah), Stress Oksidatif + Pakan I (Induksi Jelantah + Mie Instan Terigu), Stress Oksidatif + Pakan II (Induksi Jelantah + Mie Kering Gembili), Stress Oksidatif + Pakan III (Induksi Jelantah + Mie Instan Gadung), Stress Oksidatif + Pakan IV (Induksi Jelantah + Mie Kering Ubi Kelapa), Stress Oksidatif + Pakan V (Induksi Jelantah + Mie Instan Garut ),Dan Stress Oksidatif + Pakan VI (Induksi Jelantah + Mie Instan Kimpul). Pengambilan Organ Hepar Dilakukan 28 Hari Setelah Perlakuan Selesai Diberikan. Data Hasil Penelitian Dianalisis Statistik Ragam (ANOVA) Diikuti Uji Lanjut DMRT (Α=0,05). Berdasarkan Hasil Penelitian, Jenis Perlakuan Memberikan Pengaruh Yang Nyata (Α=0,05) Terhadapt Kadar MDA, SOD Dan Aktivitas Katalase Organ Hepar. Pada Parameter Aktivitas SOD Organ Hepar, Aktivitas SOD Terbesar Ditunjukan Oleh Kelompok Perlakuan Hepatoprotektor 3 (Mie Isntan Gadung) Dengan Aktivitas SOD Sebesar 79.24 U/G . Aktivitas SOD Terkecil Yakni Pada Perlakuan Hepatoprotektor I (Mie Instan Terigu) Dengan Aktivitas SOD Sebesar 47.52 U/G. Kadar MDA Terkecil Ditunjukan Pada Perlakuan Hepatoprotektor III (Mie Instan Gadung) Sebesar 6.07 Unit/Ml. Kadar MDA Terbesar Yakni Pada Perlakuan Hepatoprotektor I (Mie Instan Terigu) Yakni Sebesar 16.20 Unit/Ml. Aktivitas Katalase Terbesar Yakni Pada Perlakuan Hepatoprotektor III (Mie Instan Gadung), Hepatoprotektor V (Mie Instan Garut), Hepatoprotektor VI (Mie Instan Kimpul) Sebesar 0,047 Mm/Menit/Mg. Aktivitas Katalase Terkecil Yakni Pada Perlakuan Hepatoprotektor I (Mie Instan Terigu) Sebesar 0,002 Mm/Menit/Mg.
English Abstract
Chronic Liver Function Impairment Caused By Damage To One Cell Caused By The Oxidative Stress. Oxidative Stress Arises As A Result Of Exposure From Free Radicals In The Body Which Can Be Sourced From The Induction Of Oxidized Frying Oil. Things Like This Will Lead To The Breakdown Of Fats, Fat Peroxydation Molecules (Phospholipids) Was Associated With Cell Membranes, DNA And Protein Oxidation. Local Tubers As Arrow Root, Lesser Yam, Water Yam, Wild Yam, Coco Yam That Contain Dioscorin, Diosgenin And Phenol Are Believed To Function As Antioxidants. Antioxidants Can Counter The Influence Of Free Radicals. On The Other Hand, This Tubers Rarely Processed And Consumed By The Community Because It Feels Less Favored. Therefore Local Tubers Is Processed Into Healthy Noodles. This Noodles Are Expected To Be An Alternative Functional Foods For The Community. The Study Aimed To Determine The Protection Liver Effects Of Healthy Noodles From The Tubers Against MDA, SOD, Catalase Activitiy Of Rats Liver Organs And Histipathology Image From The Oxidative Stress Rats That Cause By The Broken Oil. The Design Of This Study Is Randomized Complete Design (RCD) With One Factor, It Consisted Of Eight Levels And Repeated 5 Times. The Levels Consisted Of The Negative Control Group (Normal), Positive Control Group (Oxidative Stress), Oxidative Stress With 1st Group (Oxidized Frying Oil + Instant Wheat Noodle), Oxidative Stress With 2nd Group (Oxidized Frying Oil + Dry Lesser Yam Noodle), Oxidative Stress With 3rd Group (Oxidized Frying Oil + Instant Wild Yam Noodle), Oxidative Stress With 4th Group (Oxidized Frying Oil + Dry Water Yam Noodle), Oxidative Stress With 5th Group (Oxidized Frying Oil + Instan Arrow Root Noodle), And Oxidative Stress With 6th Group (Oxidized Frying Oil + Instan Cocoyam Noodle). The Removal Of Liver Organs Is Done 28 Days After Treatment. The Data Be Analyzed By Analysis Of Variance (ANOVA) Followed By DMRT Test (Α=0,05). Based On The Results Of Research, A Kind Of Treatment Give Real Impact (Α=0.05) To Liver MDA Levels, SOD Levels, And Catalase Activity. On The Liver’s SOD Activity Parameter, The Largest SOD Activity Levels Indicated By The Hepatoprotector 3 (Cocoyam Instant Noodles) Treatment Group With 79.24 U/G SOD Activity Levels. The Smallest SOD Activity Levels Indicated By Hepatoprotector 1 Group (Instant Noodle) With 47.52 U/G. The Largest MDA Levels Indicated By Hepatoprotektor 3 (Wild Yam Instant Noodle) Group With 6.07unit/Ml. The Smallest MDA Levels Indcated By Hepatoprotector 1 Group (Instant Noodle) With 16.20 Unit/Ml. The Largest Catalase Activity Decrease Indicated By Hepatoperotector 3 Group (Wild Yam Instant Noodle), Hepatoprotector 5 Group (Instant Arrow Root Noodle), And Hepatoprotector 6 Group (Instant Cocoyam Noodle) With 0,0047 Mm/Minute/Mg. The Smallest Catalase Activity Decrease Indicated By Hepatoprotector 1 Group (Instant Noodle) With 0,002 Mm/Minute/Mg.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FTP/2015/141/051503479 |
| Subjects: | 300 Social sciences > 338 Production > 338.1 Agriculture |
| Divisions: | Fakultas Teknologi Pertanian > Teknologi Hasil Pertanian |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 28 May 2015 14:37 |
| Last Modified: | 15 Nov 2021 04:47 |
| URI: | http://repository.ub.ac.id/id/eprint/150026 |
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