Isolasi dan Karakterisasi Enzim Mananase dari Bakteri Mananolitik TP4.

Sigres, Divan Probo (2014) Isolasi dan Karakterisasi Enzim Mananase dari Bakteri Mananolitik TP4. Sarjana thesis, Universitas Brawijaya.

Abstract

Manan merupakan polisakarida yang banyak ditemukan di alam serta sumber yang murah untuk produksi manosa dan manooligosakarida. Hidrolisis manan dapat dilakukan secara enzimatis. Pemanfaatan enzim pada industri lebih disukai karena dapat mengurangi penggunaan bahan kimia dan ramah lingkungan. Enzim yang dapat dimanfaatkan pada industri pakan maupun pangan salah satunya adalah enzim mananase. Enzim mananase dapat diproduksi dari tanaman ataupun mikroorganisme. Mikroorganisme lebih dipilih karena biaya yang lebih murah, proses produksi cepat dan mudah dimodifikasi. Penelitian ini bertujuan untuk mendapatkan isolat tanah penghasil enzim mananase (isolat mananolitik). Tujuan kedua yaitu melakukan pemurnian sederhana untuk meningkatkan aktivitas dan lama penyimpanan enzim. Tujuan ketiga yaitu melakukan karakterisasi enzim hasil pemurnian sederhana. Metode penelitian yang digunakan adalah deskriptif kuantitatif. Tahapan penelitian meliputi isolasi mikroorganisme penghasil enzim, produksi enzim, pemurnian sederhana enzim, dan karakterisasi enzim mananase. Empat isolat mananolitik yang berhasil diperoleh dari penelitian ini yaitu TP4, TP5, P4, dan TP4 S. Isolat TP4 dipilih karena memiliki indeks mananolitik tertinggi yakni 11. Isolat TP4 memiliki morfologi bentuk koloni bulat, bentuk tepi halus, warna koloni putih, dan elevasi cembung. Isolat TP4 merupakan Gram positif dan tidak menghasilkan endospora. Setelah dilakukan elektroforesis dan zimografi, enzim mananase hasil pengendapan garam amonium sulfat fraksi 70% dan dialisis memiliki berat molekul 37,15 kDa. Tingkat kemurnian enzim hasil dialisis 19,33 kali dengan aktivitas optimal pada pH 6, suhu 400C. Kestabilan enzim pada suhu 30-500C dengan pH 6-7. Aktivitas enzim ditingkatkan dengan penambahan ion logam AlCl3, BaCl2, FeSO4 dan dihambat oleh CaCl2, NaCl, MgCl2, EDTA, CuSO4, dan ZnCl2.

English Abstract

Mannan is abundant polysaccharides that found in nature and cheap source to produce mannosa ang mannooligosccharides. Mannan can be hydrolized by enzymes. Enzymes utilization at industry is more desire because can reduce of chemicals and more environmental friendly. Mannanase is an enzyme that can be utilized in food and feed industry. Mannanase can be produced by plants or microorganisms. Microbial sources are preferrred due to the low cost, ease modification and fast in production process. This study was aimed at isolating mannanase producing microorganism (mannanolytic isolate). The second aim was partial purification of crude enzyme. The third aim was characterizing mannanase obtained from partial purification. Method in this study was descriptive. Steps of this study were isolation of mannanase producing microorganism, production, partial purification, and characterization of enzyme. In this study four isolates obtained were TP4, TP5, P4, and TP4 S. Isolate TP4 was chosen with mannanolytic index of 11. The characterization of isolate TP4 were circle colony shape, smooth edge, white colony, curve elevation shape, Gram positive and non-endospore. After electrophoresis and zymogram, mannanase obtained from precipitation by 70% ammonium sulphate and dialysis has molecular weight of 37,15 kDa. The purity enzyme from partial purification was 19,33 fold with optimal activity at 400C and pH 6. Enzyme was stable at 30-500C and pH 6-7. Enzyme activity was enhanced with the presence of AlCl3, BaCl2, FeSO4 and blocked by CaCl2, NaCl, MgCl2, EDTA, CuSO4, and ZnCl2.

Item Type:	Thesis (Sarjana)
Identification Number:	SKR/FTP/2014/379/051406241
Uncontrolled Keywords:	enzim, enzim mananase, isolasi, karakterisasi,-enzyme, mannanase, isolation, characterization
Subjects:	300 Social sciences > 338 Production > 338.1 Agriculture
Divisions:	Fakultas Teknologi Pertanian > Teknologi Hasil Pertanian
Depositing User:	Budi Wahyono Wahyono
Date Deposited:	22 Oct 2014 07:55
Last Modified:	30 Nov 2021 03:47
URI:	http://repository.ub.ac.id/id/eprint/149784

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