Ayulian, Winda Hermin (2018) UjiEfektivitas Salep Kitosan Cangkang Rajungan (Portunuspelagicus) Dibandingkan dengan Salep Komersial terhadap Ekspresi Epidermal GrowthFactor (EGF) danKetebalanEpidermis Tikus (Rattusnorvegicus) Model Luka Bakar. Sarjana thesis, Universitas Brawijaya.
Abstract
Luka bakarmerupakansalahsatu trauma yang seringterjadidalamkehidupanseharihari.Beberapapenelitianmenunjukkanbahwaekstrakcangkangrajunganyang mengandungkitosandapatdigunakansebagaibahanpengobatanluka.Penelitianinibert ujuanuntukmengetahuiujiefektivitassalepkitosanekstrakcangkangrajungan (Portunuspelagicus) dibandingandengansalepkomersial(neomycin sulfate danekstrakplacenta)terhadapketebalan epidermis sertaekspresi EGF (Epidermal Growth Factor)padakulittikusmodel lukabakar. Tikus yang dipakaidalampenelitianiniadalahjenistikusputih (Rattusnorvegicus) galurWistarjantanberumur 2-3 bulandengandiberikanperlakuanlukabakarderajat II dalampadakulittikus.Tikusdibagidalam 2 kelompokterapisalepkitosandansalepkomersial yang masing-masingterdiridari 12 ekortikus, terbagidalam 3 sub kelompokdenganpemberianterapipadaharike 1, ke 3, dan ke-7.Ketebalan epidermis diamatisecarahistopatologidenganpewarnaanHematoksilin Eosin (HE) danekspresi EGF diukurdenganmenggunakanmetodeimunohistokimia.Analisa data dilakukandenganujiIndependent Ttest(α=0,05).Hasilpenelitianinimenunjukkanpemberiansalepkitosandaricangkangr ajungan (Portunuspelagicus) tidakberbedanyatadengansalepkomersialterhadappeningkatanekspresi EGF danketebalan epidermismulaihari ke-3.Kesimpulandaripenelitianinisalepkitosan 5%dapatdigunakansebagaialternatifterapilukabakar
English Abstract
Burn is one of the most common trauma found in daily life. Several studies have shown that chitosan contained in crab shell is available to be used as wound treatment material. This study aims to determine the effectiveness of chitosan ointment extracted from crabs (Portunuspelagicus) then compared to commercial ointment (neomycin sulfate and placenta extract) in increasing epidermal thickness and EGF (Epidermal Growth Factor) expression on rat skin in burn model. The rat used in this study were white male rat (Rattusnorvegicus) Wistar strain aged 2-3 months given second stadium burns on their skin. Rats population was divided into 2 groups, each group consists of 12 rats. A group was treated with chitosan ointment and one other was treated with commercial ointment. The groups were divided into 3 subgroups with therapy on day 1, 3, and 7th. The thickness of the epidermis was observed histopathologically by Hematoxylen Eosin (HE) staining and EGF expression was measured using immunohistochemical methods. Data analysis was performed in Independent Ttest (α = 0,05). The results of this study showed that chitosan ointment extracted from crabs (Portunuspelagicus) shell had no significant difference with commercial ointment in increasing EGF expression and epidermal thickness from day 3. It can be concluded that 5% chitosan ointment can be used as alternative burns therapy
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/139/051808952 |
| Uncontrolled Keywords: | Epidermis,Growth Factor, Kitosan, Luka Bakar,SalepKomersial.-Chitosan, Commercial Ointment, Burn, Epidermis, Growth Factor. |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.1 Injuries and wounds > 617.11 Burns and scalds |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng Moelyono |
| Date Deposited: | 21 Feb 2019 07:01 |
| Last Modified: | 22 Oct 2021 03:31 |
| URI: | http://repository.ub.ac.id/id/eprint/14143 |
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