Tamim, Afier Jinda (2018) Penapisan Bakteri Antagonis Dari Filosfer Tumbuhan Rumput Di Ub Forest Sebagai Agens Biokontrol Penyakit Bulai Pada Jagung Manis. Sarjana thesis, Universitas Brawijaya.
Abstract
Jagung merupakan salah satu dari lima komoditas yang menjadi program utama pemerintah dalam swasembada yang berkelanjutan. Permasalahan dari usaha peningkatan produksi dalam budidaya jagung adalah adanya penyakit bulai yang diketahui paling merugikan. Beberapa bakteri filosfer telah diketahui bermanfaat dalam hal menunjang pertumbuhan tanaman misalnya kemampuan dalam memproduksi senyawa antifungi. UB Forest merupakan hutan yang memiliki kekayaan biodiversitas tanaman yang tinggi. Oleh karena itu, pada penelitian ini akan dilakukan isolasi dan identifikasi bakteri filosfer hasil eksplorasi dari tumbuhan rumput di UB Forest untuk menekan penyakit bulai. Penelitian ini dilaksanakan pada bulan Januari 2017 hingga Juli 2018 di Laboratorium Penyakit Tumbuhan, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya dan lahan percobaan Fakultas Pertanian yang berlokasi di Kelurahan Jatimulyo, Malang. Metode penelitian menggunakan rancangan acak kelompok dengan 25 perlakuan dan 3 ulangan. Perlakuan yang digunakan ialah 22 bakteri filosfer dari 3 tanaman hasil eksplorasi di UB Forest, terdapat tiga perlakuan kontrol yakni kontrol fungisida berbahan aktif dimetamorf 50%, kontrol PGPR (Plant Growth Promotting Rhizobacteria) dan kontrol air. Masing-masing perlakuan diaplikasikan ke tanaman jagung varietas SBY yang rentan bulai dengan cara disemprot dengan volume 200 ml. Berdasarkan hasil identifikasi morfologi jamur patogen secara mikrokospis yang dapat menimbulkan penyakit bulai disebabkan oleh Peronosclerospora maydis. Hasil persentase kejadian penyakit menunjukkan bahwa tidak semua bakteri filosfer mampu menekan penyakit bulai, Perlakuan kontrol fungisida dan bakteri filosfer dengan kode isolat F, O, dan T yang teridentifikasi sebagai Bacillus sp. dan Erwinia sp. menunjukkan hasil berbeda nyata dari perlakuan kontrol air sehingga, genus bakteri filosfer tersebut dapat digolongkan menjadi agens hayati yang mampu menekan penyakit bulai. Bakteri filosfer tidak mampu mempengaruhi pertumbuhan tanaman dan jumlah daun serta tidak mampu meningkatkan berat basah dan berat kering tanaman jagung
English Abstract
Corn is one of the five commodities that are the government's main program in sustainable self-sufficiency. The problem efforts to increase production in corn cultivation is the presence of downy mildew which is known to be the most detrimental disease. Some phyllospheric bacteria have been known to be useful in supporting plant growth such as the ability to produce antifungal compounds. UB Forest is a forest that has high plant biodiversity. Therefore, in this study isolation and identification of phyllospheric bacteria will be carried out from the exploration of grass plants at UB Forest to suppress the growth of downy mildew. This research was conducted from January 2017 to July 2018 at the Laboratory of Plant Diseases, Department of Plant Pests and Diseases, Faculty of Agriculture, Brawijaya University and the experimental field of the Faculty of Agriculture located in Jatimulyo Village, Malang. The research method used a randomized block design with 25 treatments and 3 replications. The treatment used was 22 phyllosphere bacteria from 3 exploratory plants in UB Forest, there were three control treatments namely control of dimetamorf 50% active fungicide, Plant Growth Promoting Rhizobacteria (PGPR) and water control. Each treatment was applied to the SBY variety maize by spraying with a volume of 200 ml. Based on the results of identification of the microcospic morphology of pathogens that can cause downy mildew caused by Peronosclerospora maydis. The results of disease insidence percentage showed that not all phyllospheric bacteria were able to suppress the growth of downy mildew, the treatment of phyllosphere and fungicide control. Bacteria with codes of isolates F, J, O, S and T identified as Bacillus sp., Pantoea sp. and Erwinia sp. showed the results were significantly different from the treatment of water control so that the genus of the phyllospheric bacteria could be classified into biological agents that could suppress the growth of downy mildew. Phyllospheric bacteria with code of isolates I and P can influence plant growth so that they can be classified as PGPR. Where as in the parameters of the number of leaves of the phyllospheric bacteria showed an unreal difference. The results of the wet weight and dry weight of the plants showed that the phyllospheric bacteria isolate F showed higher results than other treatments, isolate F was identified as Bacillus sp. This bacterium is able to produce growth regulators so that it increases crop productivity
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2018/813/051810948 |
| Uncontrolled Keywords: | Penyakit jagung manis |
| Subjects: | 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Nur Cholis |
| Date Deposited: | 13 Mar 2019 02:05 |
| Last Modified: | 19 Oct 2021 16:23 |
| URI: | http://repository.ub.ac.id/id/eprint/13957 |
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