Aziizalita, Seruni Ummi (2018) Analisa Kekerabatan Rusa Bawean (Axis Kuhlii) Di Taman Safari Indonesia Ii Prigen Berdasarkan Sekuen Gen Cyt-B Dengan Metode Polymerase Chainreaction. Sarjana thesis, Universitas Brawijaya.
Abstract
Rusa Bawean adalah satwa endemik Indonesia yang memiliki status critically endangered. Penelitian tentang kekerabatan salah satunya menggunakan marker mtDNA dikarenakan sifat yang hanya diturunkan dari induk betina. Gen Cyt-b yang merupakan bagian dari mtDNA, baik digunakan untuk determinasi kekerabatan filogenetik pada berbagai spesies. Penelitian ini bertujuan untuk mengetahui tingkat kekerabatan antar individu rusa Bawean dan sekaligus ingin mengetahui jarak genetik antara rusa Bawean dengan rusa Babi dari data NCBI menggunakan sekuen gen Cytb. DNA didapatkan dari sampel wholeblood rusa Bawean di Taman Safari Indonesia II Prigen. Sampel rusa Bawean di Taman Safari Indonesia II Prigen yang diambil berjumlah 4 ekor, dimana diambil dari 31 populasi rusa Bawean disana. Sampel wholeblood diisolasi DNA menggunakan QIAamp® DNA Mini Kit. Primer yang digunakan dalam metode PCR adalah primer forward (CYTB_F)5’- GACAAAGCAACCTTAACCCG-3’ dan primer reverse (CYTB_R)5’- CGTTGTTTGGATGTGTGGAG-3’. Hasil PCR dilakukan sekuensing dengan metode Sanger. Hasil sekuensing dianalisa menggunakan program Bioedit dan NCBI BLAST dengan accession number HQ893538.1 untuk rusa Bawean dan MF435989.1 untuk rusa Babi. Hasil penelitian jarak genetik antara HQ893538.1 dengan keempat sampel rusa Bawean dan antar individu sampel rusa Bawean berada dibawah 2% dengan rentang 0,3-1,38%, dimana rentang tersebut untuk perbandingan pada mamalia. Jarak genetik interspesies antara rusa Babi (MF435989.1) dan keempat sampel rusa Bawean mendapatkan hasil berada di rentang 0,93-10,31%. Sequence diversity intraspesies pada sampel rusa Bawean berada pada rentang 0,25-2,74% rentang tersebut didapatkan dari penelitian sebelumnya pada rusa Sambar, dan interspesies antara sampel rusa Bawean dengan rusa Babi (MF435989.1) berada dibawah standar reverensi yaitu dibawah 5,97%. Kesimpulan penelitian ini, jarak genetik intraspesies keempat rusa Bawean Taman Safari Indonesia II Prigen berada dibawah 2%. Berdasarkan jarak genetik interspesies dan posisi pohon filogenetik, rusa Bawean TSI berbeda kekerabatan dengan rusa Babi
English Abstract
Bawean deer are endemic species of Indonesia that have critically endangered status. Research on relationship often uses mtDNA markers because of the only inherited nature of the female parent. The Cyt-b gene, which is part of mtDNA, is best used for the determination of phylogenetic kinship in various species. This study aims is to determine the level of relationship between individuals Bawean deer and at the same time wanted to know the genetic distance between Bawean deer with Hog deer from NCBI data using Cyt-b gene sequence. DNA was obtained from a whole blood sample of Bawean deer which come from Taman Safari Indonesia II Prigen. The sample of Bawean deer in Taman Safari Indonesia II Prigen taken 4 individuals, which was taken from 31 population of Bawean deer there. The whole blood sample was isolated using QIAamp® DNA Mini Kit. The primary used in the PCR method is the forward-primer (CYTB_F) 5'-GACAAAGCAACCTTAACCCG-3 'and reverse primer (CYTB_R) 5'-CGTTGTTTGGATGTGTGGAG-3'. PCR results are then sequenced using the Sanger method. The sequencing results were analyzed using Bioedit and NCBI BLAST program with accession number HQ893538.1 for Bawean deer and MF435989.1 for Hog deer. The results of the genetic distance study between HQ893538.1 with the four Bawean deer samples and between individual Bawean deer samples were below 2% with a range of 0.3 to 1.38% where the range is for comparison in mammals.. The genetic distance of interspecies between Hog deer (MF435989.1) and the four Bawean deer samples obtained results were in the range 0.93-10.31%. Sequence diversity of intraspecies on Bawean deer was in the range of 0.25-2.74% this range was obtained from previous research on Sambar deer, and the interspecies between Bawean deer sample with Deer (MF435989.1) were below the standard of reverence under 5.97%. In conclusion, the intraspecies genetic distance of the four Bawean deer of Taman Safari Indonesia II Prigen was below 2%. Based on the genetic distance of interspecies and phylogenetic tree positions, Bawean deer and Hog deer are distinct species.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/172/051811056 |
| Uncontrolled Keywords: | Rusa Bawean, mtDNA, Cyt-b, PCR-Bawean deer, mtDNA, Cyt-b, PCR |
| Subjects: | 500 Natural sciences and mathematics > 599 Mammalia (Mammals) > 599.6 Ungulates > 599.65 Cervidae (Deer) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 22 Nov 2018 03:00 |
| Last Modified: | 22 Oct 2021 03:55 |
| URI: | http://repository.ub.ac.id/id/eprint/13897 |
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