Hariadi, Cukup (2018) Potensi Bakteri Pada Serasah Tanaman Pinus Di Ub Forest Sebagai Bioremediator Bagi Tanaman Jagung Tercemar Herbisida Glifos. Sarjana thesis, Universitas Brawijaya.
Abstract
Herbisida adalah pestisida yang sering digunakan petani untuk mengendalikan gulma. Namun, penggunaan herbisida glifosat yang berlebih dapat menyebabkan penumpukan residu dan pada akhirnya akan berakibat bagi terjadinya degradasi lahan. Salah satu cara untuk menanggulangi kerugian petani akibat degradasi lahan yang disebabkan herbisida glifosat dapat dilakukan dengan bioremediasi. Tanaman pinus yang terdapat di UB Forest menyumbangkan serasah yang sangat melimpah namun kurang dimanfaatkan. Penelitian ini bertujuan untuk mengkaji potensi bakteri dari serasah tanaman pinus sebagai bioremediator lahan tercemar herbisida glifosat. Penelitian dilaksanakan di Laboratorium Penyakit Jurusan Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya dan Green House Fakultas Pertanian Universitas Brawijaya selama 5 bulan dari bulan Februari 2018 - Juni 2018. Tahapan penelitian yaitu pengambilan serasah tanaman pinus, eksplorasi dan isolasi bakteri, seleksi bakteri secara in vitro, uji efektivitas bakteri pendegradasi herbisida terhadap tanaman jagung secara in vivo, dan identifikasi bakteri terseleksi. Hasil eksplorasi bakteri dari serasah tanaman pinus didapatkan 48 isolat bakteri. Pada tahap seleksi 19 isolat bakteri mampu hidup pada medium mengandung herbisida glifosat dan hasil uji hipersensitif pada tanaman tembakau menunjukkan bahwa 10 isolat bakteri tidak bersifat patogen. Identifikasi bakteri diketahui bahwa isolat P8A, P8B dan P8E yaitu Pantoea sp., isolat P8C, P8E, P8F, P8K, P9G yaitu Erwinia sp. dan isolat P8O yaitu Pseudomonas sp. Hasil dari semua variabel pertumbuhan yang diamati meliputi tinggi tanaman, panjang akar, berat basah dan berat kering perlakuan dengan menggunakan isolat bakteri P8O menunjukkan hasil yang berbeda nyata dibandingkan dengan perlakuan menggunakan isolat bakteri lainnya dan kontrol herbisida glifosat.
English Abstract
A herbicide is pesticides that were often used to control weeds farmers. However, the use of a herbicide glifosat who waste by movements are thought to cause the accumulation of a residue and finally impact of land degradation The solution to resolve the problem of farmers loss income caused by the decreased of land productivity because the use of glifosat herbiside is to do bioremediation. Pine tree at UB forest give so many litters without being used. The purpose of this research is to study the benefit of isolate from pine’s litters as bioremediator for land that polluted by glifosat herbiside. The research was conducted at Labolatory of desease in Pest and Desease of Plant, Faculty of Agriculture, University of Brawijaya and Faculty of Agriculture’s Green House University of Brawijaya for 5 months from February 2018 until June 2018. The steps of this research are: Collecting pine litters at UB Forest, exploration and isolate the bacteria, bacteria selection at in vitro condition, effectiveness test for degradate herbiside to the growth of corn plant at in vivo condition and identified the selected bacteria. The result of bacteria exploration from pine litters get 48 bacteria isolates. In the selection of 19 isolates bacteria, the isolates can life in the medium which contain glifosat herbiside and the results of hypersensitive that test in tobacco plants shows that 10 isolates bacteria among them are not pathogen. Based on bacteria identification, the isolates known as P8A, P8B, P8E is Pantoea sp. The isolates in P8C, P8E, P8F, P8K, P9G is Erwina sp. and the isolate P8O is Pseudomonas sp. The observation result of plant growth variable which is higher plants , long roots , a heavy wetness and heavy dry treatment shows that the isolate P8O has significant result compared to the others isolates variable and glifosat herbiside control.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2018/713/051810360 |
| Uncontrolled Keywords: | A herbicide is pesticides that were often used to control weeds farmers. |
| Subjects: | 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Nur Cholis |
| Date Deposited: | 11 Mar 2019 03:01 |
| Last Modified: | 19 Oct 2021 15:03 |
| URI: | http://repository.ub.ac.id/id/eprint/13537 |
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